Monitoring of Porcine circovirus (PCV) types 2 and 3 in some large-scale pig farms in Xinjiang

doi:10.6048/j.issn.1001-4330.2025.02.028

Xinjiang Agricultural Sciences ›› 2025, Vol. 62 ›› Issue (2): 502-509.DOI: 10.6048/j.issn.1001-4330.2025.02.028

• Prataculture·Animal Husbandry Veterinarian·Agricultural Eeconomy • Previous Articles Next Articles

Monitoring of Porcine circovirus (PCV) types 2 and 3 in some large-scale pig farms in Xinjiang

QI Weiyi¹(), XU Heng², LIU Yingyu¹(), ZHENG Pei²(), XIA Lining¹, LIU Junfei²

1. College of Animal Medicine, Xinjiang Agricultural University, Urumqi 830052, China
2. Xinjiang Tekon Animal Husbandry Science and Technology Co.Ltd.,Changji Xinjiang 831100, China

Received:2024-08-05 Online:2025-02-20 Published:2025-04-17
Correspondence author: LIU Yingyu, ZHENG Pei
Supported by:
Project for Silk Road Economic Belt Pilot Zone for Innovation-driven Development Zone: Urumqi, Changji and Shihezi Independent Innovation Demonstration Zone S & T Development“High-Quality Livestock Products Industrial Cluster“Xinjiang Live Pig Industry Chain Key Technology R & D and Integration Demonstration”(2022LQ01003);"Tianshan Talents" Training Program " the "Three Rural Issues" Backbone Training Project of Xinjiang Uygur Autonomous Region“Pathogen Detection and Clinical Drug Use Guidance of Common Respiratory Tract Bacterial Diseases in Large-Scale Pig Farms in Xinjiang”

新疆部分规模化养猪场中猪圆环病毒2型和3型监测

齐炜祎¹(), 许衡², 刘英玉¹(), 郑培²(), 夏利宁¹, 刘俊飞²

1.新疆农业大学动物医学学院,乌鲁木齐 830091
2.新疆天康畜牧科技有限公司,新疆昌吉 831100

通讯作者: 刘英玉,郑培
作者简介:齐炜祎(1997-),男,河北邢台人,硕士研究生,研究方向为病原分子流行病学,(E-mail)2303092569@qq.com
基金资助:
丝绸之路经济带创新驱动发展试验区、乌昌石国家自主创新示范区科技发展项目-优质畜产品产业集群-新疆生猪产业链关键技术研发及集成示范(2022LQ01003);“天山英才”培养计划“三农”骨干人才培养专项“新疆规模化猪场常见呼吸道细菌性疾病的病原检测及临床用药指导”

Abstract

Abstract:

【Objective】To investigate the prevalence of Porcine circovirus type 2 (PCV2) and Porcine circovirus type 3 (PCV3) in Xinjiang. 【Methods】From October 2022 to September 2023, 1403 samples from 7 large-scale pig farms were detected and sequenced by fluorescent quantitative PCR. 【Results】The overall positive rate of PCV2 was 3.20%(45/1403), the overall positive rate of PCV3 was 33.00%(463/1403), and the combined infection rate of PCV2 and PCV3 was 7.84%(11/1403). The positive detection rate of PCV2 and PCV3 in C field was the highest, with PCV2 7.46%(10/134), PCV3 64.18%(36/134), and PCV2 and PCV3 mixed infection rate 3.73%(5/134). The positive rate of PCV2 and PCV3 was the highest, and the positive rate of PCV2 was 4.78%(25/523). The detection rate of PCV3 was 57.36%(300/523). The detection rates of PCV2 and PCV3 were high in all seasons, and the PCV3 strain was PCV3b and PCV3c. 【Conclusion】PCV3 infection has been found in some large-scale pig farms in Xinjiang and the prevalence rate of PCV3 is much higher than that of PCV2, and PCV3 strain is PCV3b and PCV3c.

Key words: Porcine circovirus type 3; Porcine circovirus type 2; fluorescent quantitative PCR; pig

摘要：

【目的】分析新疆圆环病毒2型(Porcine circovirus type 2,PCV2)和3型(Porcine circovirus type 3,PCV3)的流行状况。【方法】在2022年10月~2023年9月,采用荧光定量 PCR对采集的7个规模化养猪场的1 403份样品进行检测和测序。【结果】PCV2总体阳性率为3.20%(45/1403),PCV3总体阳性率为33.00%(463/1403),PCV2和PCV3混合感染率为7.84%(11/1403)。C场PCV2和PCV3阳性检出率最高,PCV2 为7.46%(10/134),PCV3为64.18%(36/134),PCV2和PCV3混合感染率为3.73%(5/134)。乳猪睾丸液检出PCV2和PCV3阳性率最高,PCV2 阳性率为4.78%(25/523);PCV3 检出率为57.36%(300/523)。PCV2和PCV3在一年四季中检出率均较高,且PCV3毒株为PCV3b和PCV3c型。【结论】新疆部分规模化养猪场中存在PCV3感染,PCV3流行率远高于PCV2,PCV3流行毒株为PCV3b和PCV3c型。

关键词: 圆环病毒3型, 圆环病毒2型, 荧光定量PCR, 猪

CLC Number:

S852.3

QI Weiyi, XU Heng, LIU Yingyu, ZHENG Pei, XIA Lining, LIU Junfei. Monitoring of Porcine circovirus (PCV) types 2 and 3 in some large-scale pig farms in Xinjiang[J]. Xinjiang Agricultural Sciences, 2025, 62(2): 502-509.

齐炜祎, 许衡, 刘英玉, 郑培, 夏利宁, 刘俊飞. 新疆部分规模化养猪场中猪圆环病毒2型和3型监测[J]. 新疆农业科学, 2025, 62(2): 502-509.

Figures/Tables 8

References 23

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场区 Field	血清样品 Seroplasm	死胎样品 Stillbirth-like	睾丸液样品 Testicular fluid	脐带样品 Umbilical cordoid	组织样品 Tissue sample	精液样品 Semen-like	总计 Total
A场	38	6	165	15	31	0	255
B场	30	0	36	6	30	0	102
C场	39	12	35	14	34	0	134
D场	40	2	66	19	29	0	156
E场	28	2	84	19	38	0	171
F场	0	0	0	0	0	364	364
G场	33	0	137	13	38	0	221
总计Total	208	22	523	86	200	364	1 403

场区 Field	血清样品 Seroplasm	死胎样品 Stillbirth-like	睾丸液样品 Testicular fluid	脐带样品 Umbilical cordoid	组织样品 Tissue sample	精液样品 Semen-like	总计 Total
A场	38	6	165	15	31	0	255
B场	30	0	36	6	30	0	102
C场	39	12	35	14	34	0	134
D场	40	2	66	19	29	0	156
E场	28	2	84	19	38	0	171
F场	0	0	0	0	0	364	364
G场	33	0	137	13	38	0	221
总计Total	208	22	523	86	200	364	1 403

模板 Mould	引物 Primer	引物序列(5'→ 3') Primer sequences(5'→ 3')
PCV2	PCV2-Rep-F	GGAGTCTGGTGACCGTTGC
	PCV2-Rep-R	CCAATCACGCTTCTGCATTTT
	PCV2-Rep-P	FAM-CCGCTCACTTTCAAAAG TTCAGCCA-BHQ

模板 Mould	引物 Primer	引物序列(5'→ 3') Primer sequences(5'→ 3')
PCV2	PCV2-Rep-F	GGAGTCTGGTGACCGTTGC
	PCV2-Rep-R	CCAATCACGCTTCTGCATTTT
	PCV2-Rep-P	FAM-CCGCTCACTTTCAAAAG TTCAGCCA-BHQ

场区 Field	样本数量 Sample size	PCV2 阳性率 PCV2 Positivity rate	PCV3 阳性率 PCV3 Positivity rate	PCV2+3 阳性率 PCV2+3 Positivity rate
A场	255	1.96% (5/255)	25.49% (65/255)	0
B场	102	5.88% (6/102)	48.04% (49/102)	0
C场	134	7.46% (10/134)	64.18% (86/134)	3.73% (5/134)
D场	156	4.49% (7/156)	48.72% (76/156)	1.92% (3/156)
E场	171	5.26% (9/171)	55.56% (95/171)	1.75% (3/171)
F场	364	1.10% (4/364)	2.75% (10/364)	0
G场	221	1.81% (4/221)	37.10% (82/221)	0
总计 Total	1 403	3.21% (45/1403)	33.00% (463/1403	0.78% (11/1403)

Monitoring of Porcine circovirus (PCV) types 2 and 3 in some large-scale pig farms in Xinjiang

新疆部分规模化养猪场中猪圆环病毒2型和3型监测

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Figures/Tables 8

References 23

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Metrics

样本类型 Sample type	样本数量 Sample size	PCV2 阳性率 PCV2 Positivity rate	PCV3 阳性率 PCV3 Positivity rate	PCV2+3 阳性率 PCV2+3 Positivity rate
血清样 Seroplasm	208	1.92% (4/208)	35.10% (73/208)	0
死胎样 Stillbirth- like	22	0	13.64% (3/22)	0
睾丸液 Testicular fluid	523	4.78% (25/523)	57.36% (300/523)	3.73% (5/523)
脐带样 Umbilical cordoid	86	3.49% (3/86)	37.21% (32/86)	1.92% (3/86)
组织样 Tissue sample	200	4.5% (9/200)	25.00% (50/200)	1.75% (3/200)
精液 Semen- like	364	1.10% (4/364)	1.37% (5/364)	0
总计 Total	1 403	3.21% (45/1403)	33.00% (463/1403)	0.78% (11/1403)

血清类型 Serum type	样本数量 Sample size	PCV2 阳性率 PCV2 Positivity rate	PCV3 阳性率 PCV3 Positivity rate	PCV2+ PCV3 阳性率 PCV2+3 Positivity rate
乳猪 Suckling	31	3.23% (1/31)	48.39% (15/31)	3.23% (1/31)
仔猪 Piglet	69	2.90% (2/69)	26.09% (18/69)	0
育肥猪 Fattening pigs	63	0%	33.33% (21/63)	0
妊娠猪 Pregnant pigs	45	2.22% (1/45)	42.22% (19/45)	0
总计 Total	208	3.21% (4/208)	35.10% (73/208)	0.48% (1/73)

季度 Quarterly	样本数量 Sample size	PCV2 阳性率 PCV2 Positivity rate	PCV3 阳性率 PCV3 Positivity rate	PCV2+3 阳性率 PCV2+3 Positivity rate
第4季度 Fourth quarter	164	5.49% (9/164)	27.44% (45/164)	0.61% (1/164)
第1季度 First quarter	252	4.37% (11/252)	28.17% (71/252)	1.19% (3/252)
第2季度 Second quarter	486	2.67% (13/486)	33.95% (16/486)	0.82% (4/486)
第3季度 Third quarter	501	2.40% (12/501)	36.33% (182/501)	0.40% (2/501)