Isolation and antagonistic activity screening of Actinomycetes from rhizosphere soil of Tamarix

doi:10.6048/j.issn.1001-4330.2023.07.027

Abstract

Abstract:

【Objective】 To lay a foundation for the prevention and control of major agricultural and animal husbandry diseases in Xinjiang.【Methods】 Ten species of actinomycetes from rhizosphere soils of Tamarix ramosissima in southern Xinjiang were selected and their antibactieral activities were studied.Species diversity of actinomycetes in rhizosphere soil of Tamarix ramosissima was analyzed by 16SrDNA sequencing using four kinds of media.The antibacterial activity was screened by plate confrontation methods.The antibacterial activity was screened by plate confrontation method.【Results】 The results showed that total 294 strains were isolated, and total 150 strains were identified, which belonged to seven classes and twelve orders, total 12 families, total 24 genera, and 9 potential new species.At the same time, the antibacterial activity was analyzed with Staphylococcus aureus, Pseudonas aeruginosa, Escherichia coli, Klebsiella pneumonia, Erwinia amylohydrolytic, Slmonella typhoid, Candida albicans, Chain bacteria Verticilliuma dhiiae, Fusarium oxysporum as the target bacteria.【Conclusion】 Total 68 strains of active actinmycetes were screened by antibacterial activity analysis. Isolation of actinomycetes from rhizosphere soil of Tamarix ramosissima enriched microbial resources and species reserve.

Key words: rhizosphere soil of Tamarix; actinomycetes; species diversity

摘要：

【目的】分析柽柳根际土壤放线菌物种多样性,研究其抗菌活性,为新疆主要农牧业病害的防控奠定菌种基础。【方法】选择新疆南疆地区10种柽柳根际土壤的放线菌资源,采用四种培养基,通过菌株16SrDNA测序,采用平板对峙法筛选抗菌活性。【结果】分离出294株菌株,鉴定的150株,分属于7个纲12个目16科24个属,另有9个潜在新种,以金黄色葡萄球菌、铜绿假单胞菌、大肠杆菌、肺炎克雷伯菌、解淀粉欧文氏菌、伤寒沙门氏菌、白色念珠菌、链格孢菌、棉花黄萎病菌、棉花枯萎病菌为靶标菌。【结论】筛选出68株活性放线菌。柽柳根际土壤放线菌的分离丰富了微生物资源及菌种的储备。

关键词: 柽柳根际土壤, 放线菌, 物种多样性

CLC Number:

CHEN Yihuang, XING Li, DONG Zhenzhen, MA Xiaomei, HUANG Jianjun, LUO Xiaoxia. Isolation and antagonistic activity screening of Actinomycetes from rhizosphere soil of Tamarix[J]. Xinjiang Agricultural Sciences, 2023, 60(7): 1790-1797.

陈乙煌, 邢利, 东珍珍, 马小梅, 黄建军, 罗晓霞. 柽柳根际土壤放线菌的分离及拮抗活性筛选[J]. 新疆农业科学, 2023, 60(7): 1790-1797.

Figures/Tables 5

References 21

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土样编号 Soil sample number	样品类型 Soil sample type	取样位置 Sampling location	纬度 Latitude (°)	经度 Longitude (°)	海拔 Altitude (m)
HT44	红柳根际土	塔中-民丰	38.412	83.205	1 164.9
HT51	红柳根际土	萨勒吾则克乡	37.254	83.018	1 386.6
HT53	红柳根际土	民丰-且末	37.688	83.959	1 341.1
HT57	红柳根际土	塔提让乡	38.485	85.824	1 112.1
HT61	红柳根际土	瓦石峡乡	38.819	87.743	885.2
HT65	红柳根际土	若羌-库尔勒	39.889	88.399	814.3
HT67	红柳根际土	大西海子水库	40.562	87.810	829.6
HT71	红柳根际土	沙雅	41.137	82.446	957.8
HT75	红柳根际土	二牧场-14团	40.660	81.943	975.1
HT49	红柳根际土	民丰-于田	36.888	82.553	1 664.8

土样编号 Soil sample number	样品类型 Soil sample type	取样位置 Sampling location	纬度 Latitude (°)	经度 Longitude (°)	海拔 Altitude (m)
HT44	红柳根际土	塔中-民丰	38.412	83.205	1 164.9
HT51	红柳根际土	萨勒吾则克乡	37.254	83.018	1 386.6
HT53	红柳根际土	民丰-且末	37.688	83.959	1 341.1
HT57	红柳根际土	塔提让乡	38.485	85.824	1 112.1
HT61	红柳根际土	瓦石峡乡	38.819	87.743	885.2
HT65	红柳根际土	若羌-库尔勒	39.889	88.399	814.3
HT67	红柳根际土	大西海子水库	40.562	87.810	829.6
HT71	红柳根际土	沙雅	41.137	82.446	957.8
HT75	红柳根际土	二牧场-14团	40.660	81.943	975.1
HT49	红柳根际土	民丰-于田	36.888	82.553	1 664.8

序号	菌株编号	相似菌株	相似性(%)	抑菌圈直径类型(mm)
序号	菌株编号	相似菌株	相似性(%)	S.a	P.a	E.c	K.p	E.a	S.t	C.a	A.t	V.d	F.o
1	TRM 76131	Cellulosimicrobium marinum RS-7-4	98.99	—	—	+	—	+	+	++	—	+++	+
2	TRM 76130	Rhizobium tarimense PL-41	99.71	++	—	+	—	—	+	+++		+++	++
3	TRM 76129	Salmonella enterica subsp.entericaL T2	99.23	—	—	—	+	+	+	++	++	++	—
4	TRM 76146	Rhizobium tarimense PL-41	99.05	—	—	+	—	+	+	—	+	+	—
5	TRM 76147	Microbacterium petrolearium LAM0410	98.56	—	+	—	—	—	—	+	+++	+++	+
6	TRM 76156	Rhizobium tarimense PL-41	98.84	—	—	—	—	—	—	+++	+++	+++	+
7	TRM 76174	Neorhizobium alkalisol CCBAU 01393i	98.84	—	—	—	+	+	—	+++	+++	+++	+
8	TRM 76175	Rhizobium rosettiformans W₃	99.06	—	—	—	+	+	—	++	++	—	—
9	TRM 76101	Pseudochrobactrum asaccharolyticum DSM 25619	99.18	—	+	++	++		++	+++	—	—	—
10	TRM 76159	Phyllobacterium myrsinacearum DSM 5892	99.41	—	—	+	+	—	+	++	—	+++	+++
11	TRM 76062	Bacillus rugosus SPB7	99.86	—	—	—	+	+	+	—	—	—	—
12	TRM 76132	Rhizobium tarimense PL-41	99.56	—	+	—	—	—	—	+	+++	++	++
13	TRM 76072	Streptomyces tunisiensis CN-207	98.98	++	+++	—	—	—	—	—	—	—	—
14	TRM 76100	Achromobacter marplatensis B2	99.71	—	+	—	+	+	—	—	+++	—	++
15	TRM 76153	Bacillus cabrialesii TE3	99.79	—	—	—	—	—	—	++	+++	+	++

序号	菌株编号	相似菌株	相似性(%)	抑菌圈直径类型(mm)
序号	菌株编号	相似菌株	相似性(%)	S.a	P.a	E.c	K.p	E.a	S.t	C.a	A.t	V.d	F.o
1	TRM 76131	Cellulosimicrobium marinum RS-7-4	98.99	—	—	+	—	+	+	++	—	+++	+
2	TRM 76130	Rhizobium tarimense PL-41	99.71	++	—	+	—	—	+	+++		+++	++
3	TRM 76129	Salmonella enterica subsp.entericaL T2	99.23	—	—	—	+	+	+	++	++	++	—
4	TRM 76146	Rhizobium tarimense PL-41	99.05	—	—	+	—	+	+	—	+	+	—
5	TRM 76147	Microbacterium petrolearium LAM0410	98.56	—	+	—	—	—	—	+	+++	+++	+
6	TRM 76156	Rhizobium tarimense PL-41	98.84	—	—	—	—	—	—	+++	+++	+++	+
7	TRM 76174	Neorhizobium alkalisol CCBAU 01393i	98.84	—	—	—	+	+	—	+++	+++	+++	+
8	TRM 76175	Rhizobium rosettiformans W₃	99.06	—	—	—	+	+	—	++	++	—	—
9	TRM 76101	Pseudochrobactrum asaccharolyticum DSM 25619	99.18	—	+	++	++		++	+++	—	—	—
10	TRM 76159	Phyllobacterium myrsinacearum DSM 5892	99.41	—	—	+	+	—	+	++	—	+++	+++
11	TRM 76062	Bacillus rugosus SPB7	99.86	—	—	—	+	+	+	—	—	—	—
12	TRM 76132	Rhizobium tarimense PL-41	99.56	—	+	—	—	—	—	+	+++	++	++
13	TRM 76072	Streptomyces tunisiensis CN-207	98.98	++	+++	—	—	—	—	—	—	—	—
14	TRM 76100	Achromobacter marplatensis B2	99.71	—	+	—	+	+	—	—	+++	—	++
15	TRM 76153	Bacillus cabrialesii TE3	99.79	—	—	—	—	—	—	++	+++	+	++