Effects of Mixed Salt and Alkali on Osmoregulation of Cotton Seedlings

doi:10.6048/j.issn.1001-4330.2021.11.006

Abstract

Abstract:

【Objective】 To study the salt tolerance response mechanism of osmotic regulators of cotton seedlings under mixed salt and alkali stress. 【Methods】 Xinluzao 57 was taken as the research object, the NaCl, Na₂SO₄, Na₂SO₃, and Na₂CO₃ 4 salts were mixed in different proportions to simulate the stress conditions of mixed salt, and the alkaline salt proportion was gradually increased into A, B, C, D and E 5 groups and treated with different concentrations. 【Results】 The results showed that there was a significant correlation between osmoregulation substance and salt alkali ratio, salt alkali concentration (P < 0.01), and the interaction between salt alkali ratio and salt alkali concentration had a significant correlation with osmoregulation substance of cotton seedling leaves; proline content increased with the increase of salt alkali concentration. The content of proline in treatment B (NaCl, NaHCO₃) was significantly higher than those in other treatments with the increase of concentration, and the difference of proline content in treatment C (NaCl, Na₂SO₄, NaHCO₃, Na₂CO₃) was lower. At the concentration of 25 mmol/L, the proline content of leaves increased the most, and with the increase of concentration, the increase of proline content decreased significantly; the change trend of malondialdehyde and soluble sugar was similar to that of free proline, while the content of soluble protein increased with the increase of treatment concentration, but the increase was small; and the difference was not significant. 【Conclusion】 The results showed that there is a significant correlation between osmotic adjustment substances and salt alkali ratio and concentration (P < 0.01), and the interaction between salt alkali ratio and salt alkali concentration has a significant correlation on osmotic adjustment substances of cotton seedling leaves. Under the same mixed salt treatments, the contents of proline, soluble sugar, malondialdehyde and free proline increase with the increase of salt concentrations. The content of soluble protein increases little with the increase of concentration, but the difference is significant in different saline alkali ratios of the same salt concentration, which further indicates that the soluble protein content of cotton seedling leaves under mixed salt alkali stress is the main osmotic adjustment substance to evaluate the salt tolerance of cotton.

Key words: mixed salt alkali; cotton seedling; osmoregulation substance

摘要：

【目的】研究棉花幼苗在混合盐碱胁迫下渗透调节物质耐盐响应机制。【方法】以新陆早57号为研究对象,将NaCl、Na₂SO₄、NaHCO₃和Na₂CO₃ 4种盐按不同比例混合进行人工模拟混合盐胁迫条件,以碱性盐比例逐步增大的顺序分成A、B、C、D、E 5组不同浓度处理。【结果】棉花幼苗叶片渗透调节物质与盐碱比例、盐碱浓度呈极显著相关(P<0.01),且盐碱比例与盐碱浓度的交互作用对棉花幼苗叶片的渗透调节物质影响呈极显著相关;随着盐碱浓度的增大脯氨酸含量均呈现上升趋势,在200 mmol/L下含量达到最大;在B处理下(NaCl、NaHCO₃)随着浓度的增大脯氨酸含量增加的趋势明显大于其他处理,且在150、200 mmol/L下含量差异较小;在C处理下(含有NaCl、Na₂SO₄、NaHCO₃、Na₂CO₃),在浓度为25 mmol/L下,叶片的脯氨酸含量增加幅度最大,其随着浓度的增加,脯氨酸含量增加幅度明显降低;丙二醛、可溶性糖变化趋势与游离脯氨酸变化趋势相似,而可溶性蛋白含量随着处理浓度的增加而增加,但增加幅度较小;且差异不显著。【结论】棉花幼苗叶片渗透调节物质与盐碱比例、盐碱浓度呈极显著相关(P<0.01),且盐碱比例与盐碱浓度的交互作用对棉花幼苗叶片的渗透调节物质影响呈极显著相关,在同一种混合盐处理下,脯氨酸、可溶性糖、丙二醛的含量、游离脯氨酸含量随着盐浓度的增加而增加;可溶性蛋白含量,随着浓度的增加,增加幅度较小,但在同一盐浓度不同盐碱比例中差异显著,混合盐碱胁迫下棉花幼苗叶片可溶性蛋白含量是评价棉花耐盐性的主要渗透调节物质。

关键词: 混合盐碱, 棉花幼苗, 渗透调节物质

CLC Number:

S562

ZHANG Mengjie, WU Xianglin, WEI Yayuan, GENG Qinglong, Halihashiebati Yibati, LI Qingjun. Effects of Mixed Salt and Alkali on Osmoregulation of Cotton Seedlings[J]. Xinjiang Agricultural Sciences, 2021, 58(11): 2011-2023.

张梦婕, 吴湘琳, 魏亚媛, 耿庆龙, 哈丽哈什·依巴提, 李青军. 混合盐碱对棉花幼苗渗透调节物质的影响[J]. 新疆农业科学, 2021, 58(11): 2011-2023.

Figures/Tables 15

References 25

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处理组	盐分组成及摩尔比				各盐浓度(mmol/L)处理组对应pH
处理组	NaCl	Na₂SO₄	NaHCO₃	Na₂CO₃	25	50	100	150	200	平均
CK	0	0	0	0	7.00	7.00	7.00	7.00	7.00	7.00
A	1	1	0	0	7.12	7.16	7.24	7.35	7.46
B	1	0	1	0	8.26	8.32	8.39	8.48	8.58
C	1	1	1	1	9.24	9.31	9.45	9.52	9.59
D	0	1	0	1	10.11	10.18	10.26	10.37	10.46
E	0	0	1	1	11.03	11.12	11.19	11.27	11.33

处理组	盐分组成及摩尔比				各盐浓度(mmol/L)处理组对应pH
处理组	NaCl	Na₂SO₄	NaHCO₃	Na₂CO₃	25	50	100	150	200	平均
CK	0	0	0	0	7.00	7.00	7.00	7.00	7.00	7.00
A	1	1	0	0	7.12	7.16	7.24	7.35	7.46
B	1	0	1	0	8.26	8.32	8.39	8.48	8.58
C	1	1	1	1	9.24	9.31	9.45	9.52	9.59
D	0	1	0	1	10.11	10.18	10.26	10.37	10.46
E	0	0	1	1	11.03	11.12	11.19	11.27	11.33

方差分析 Variance analysis	变异来源 Source of variation	平方和 SS	均方 MEAN	F
脯氨酸含量 Proline content	盐碱比例SR	40.900 9	10.225 2	7.408 9^**
	盐碱浓度SC	52.239 1	13.059 8	9.462 7^**
	比例×浓度SR×SC	93.140 0	11.642 5	8.435 8^**
丙二醛含量 MAD	盐碱比例SR	43.565 3	8.713 1	5.700 9^**
	盐碱浓度SC	14.941 9	2.988 4	1.955 3
	比例×浓度SR×SC	60.526 3	6.052 6	3.960 2^**
可溶性蛋白含量 Solubleprotein content	盐碱比例SR	0.423 0	0.084 6	7.477 5^**
	盐碱浓度SC	0.012 3	0.002 5	0.216 8
	比例×浓度SR×SC	0.438 0	0.043 8	3.872 0^**
可溶性糖含量 Soluble sugar content	盐碱比例SR	0.008 5	0.001 7	8.670 1^**
	盐碱浓度SC	0.008 2	0.001 6	8.288 6^**
	比例×浓度SR×SC	0.017 6	0.001 8	8.952 4^**

方差分析 Variance analysis	变异来源 Source of variation	平方和 SS	均方 MEAN	F
脯氨酸含量 Proline content	盐碱比例SR	40.900 9	10.225 2	7.408 9^**
	盐碱浓度SC	52.239 1	13.059 8	9.462 7^**
	比例×浓度SR×SC	93.140 0	11.642 5	8.435 8^**
丙二醛含量 MAD	盐碱比例SR	43.565 3	8.713 1	5.700 9^**
	盐碱浓度SC	14.941 9	2.988 4	1.955 3
	比例×浓度SR×SC	60.526 3	6.052 6	3.960 2^**
可溶性蛋白含量 Solubleprotein content	盐碱比例SR	0.423 0	0.084 6	7.477 5^**
	盐碱浓度SC	0.012 3	0.002 5	0.216 8
	比例×浓度SR×SC	0.438 0	0.043 8	3.872 0^**
可溶性糖含量 Soluble sugar content	盐碱比例SR	0.008 5	0.001 7	8.670 1^**
	盐碱浓度SC	0.008 2	0.001 6	8.288 6^**
	比例×浓度SR×SC	0.017 6	0.001 8	8.952 4^**

处理 T	MeanDiff	SEM	q Value	Prob	Sig
A CK	140.530	57.950	3.430	0.210	0
B CK	266.860	57.950	6.510	0.000	1
B A	126.330	33.460	5.340	0.020	1
C CK	260.980	57.950	6.370	0.000	1
C A	120.450	33.460	5.090	0.030	1
C B	-5.870	33.460	0.250	1.000	0
D CK	239.200	57.950	5.840	0.010	1
D A	98.670	33.460	4.170	0.090	0
D B	-27.650	33.460	1.171	0.960	0
D C	-21.780	33.461	0.921	0.980	0
E CK	148.480	57.950	3.620	0.170	0
E A	7.950	33.460	0.340	1.000	0
E B	-118.370	33.461	5.000	0.030	1
E C	-112.500	33.460	4.751	0.040	1
E D	-90.720	33.461	3.831	0.130	0