多浪羊与湖羊杂交产羔性状COIL及BMPR-IB基因遗传效应分析

doi:10.6048/j.issn.1001-4330.2025.01.028

摘要/Abstract

摘要：

【目的】 分析COIL及BMPR-IB基因与产羔数关联性,探究多浪羊与湖羊(简称多湖杂交)后代产羔性状COIL及BMPR-IB基因的遗传效应。【方法】 研究以多浪羊与湖羊级进杂交F₁代、F₂代、F₃代为研究对象,随机选取多湖杂交F₁代(228只)、F₂代(254只)、F₃代(252只),进行全血DNA提取,COIL基因利用扩增阻滞突变系统PCR(ARMS-PCR)技术基因分型、BMPR-IB基因利用限制性内切酶片段长度多态性技术(PCR-RFLP)基因分型后,与产羔数关联分析。【结果】 在多湖杂交F₁代中,突变纯合GG型产羔数极显著高于突变杂合CG、野生CC型产羔数(P<0.01),突变杂合CG型产羔数极显著高于野生CC型产羔数(P<0.01);在多湖杂交F₂代中突变纯合GG型与突变杂合CG、野生CC型产羔数间均差异极显著(P<0.01);在多湖杂交F₃代中,突变纯合GG、突变杂合CG型产羔数极显著高于CC型(P<0.01),GG型产羔数极显著高于CG型(P<0.05)。在多湖杂交F₁、F₂、F₃代中均为突变纯合BB型产羔数极显著高于突变杂合B+型、野生++型产羔数(P<0.01),突变杂合B+型产羔数极显著高于野生++型产羔数(P<0.01)。【结论】 COIL基因与BMPR-IB基因均对多湖杂交后代产羔数影响显著,但BMPR-IB基因在多湖杂交后代突变率较低,可能不适合作为筛选多湖杂交双羔群体的关键基因,COIL基因可以作为筛选多湖杂交高繁殖率的新候选基因。

关键词: 多浪羊; 湖羊; 杂交; 产羔性状; COIL基因; BMPR-IB基因; 遗传效应

Abstract:

【Objective】 This study aims to investigate the genetic effects of lambing traits COIL and BMPR-IB genes in the offspring of Duolang sheep and Hu sheep crossbreeding (Duohu sheep crossbreeding) cascade crosses by analyzing the association between COIL and BMPR-IB genes and the number of lambs produced. 【Methods】 Randomly selected F₁ (228), F₂ (254), and F₃ (252) generations of Duohu crossbred sheep were subjected to whole blood DNA extraction, and the COIL gene was genotyped using amplification refractory mutation system-PCR (ARMS-PCR) technique, and the BMPR-IB gene was analyzed in association with the number of lambs produced after genotyping using PCR-restriction fragment length polymorphism (PCR-RFLP) technique. 【Results】 In the Duohu F₁ sheep, the lambing numbers of the mutant pure GG type were significantly higher than those of the mutant heterozygous CG and the wild CC type (P<0.01), and that of the mutant heterozygous CG type were significantly higher than that of the wild CC type (P<0.01); in the Duohu F₂ sheep, the differences between the number of lambs produced by the mutant pure GG type and the mutant heterozygous CG and wild CC types were all highly significant (P<0.01); in the Duohu F₃ sheep, the number of lambs produced by the mutant pure GG and the mutant heterozygous CG types was highly significantly higher than that of the CC type (P<0.01), and the number of lambs produced by the GG type was highly significantly higher than that of the CG type (P<0.05). In the Duohu F₁, F₂ and F₃ sheep, the numbers of lambs produced by the mutant pure BB type were highly significantly higher than the number of lambs produced by the mutant heterozygous B+ type and the wild++ type (P<0.01), and the number of lambs produced by the mutant heterozygous B+ type was highly significantly higher than that of the wild++ type (P<0.01). 【Conclusion】 Both COIL gene and BMPR-IB gene have significant effect on lambing number of Duo and Hu crossbred sheep. However, the BMPR-IB gene has a low mutation rate in the progeny of Duohu sheep, which may not be suitable as a key gene for screening the double lambing population of Duohu sheep, and the COIL gene can be a new candidate gene for screening the high reproduction rate of Duohu sheep.

Key words: Duolang sheep; Hu sheep; crossbreed; lambing traits; COIL gene; BMPR-IB gene; genetic effects

中图分类号:

S826.6

史香云, 李焦智, 刘玲玲, 刘武军. 多浪羊与湖羊杂交产羔性状COIL及BMPR-IB基因遗传效应分析[J]. 新疆农业科学, 2025, 62(1): 243-250.

SHI Xiangyun, LI Jiaozhi, LIU Lingling, LIU Wujun. Analysis of genetic effects of COIL and BMPR-IB genes for lambing traits in Duolang sheep and Hu sheep crossbred sheep[J]. Xinjiang Agricultural Sciences, 2025, 62(1): 243-250.

图/表 16

表1 COIL基因ARMS-PCR反应程序

Tab.1 COIL gene ARMS-PCR reaction program

温度 Temperature	时间 Time	循环次数 Number of cycles
94℃预变性	5 min
94℃变性	30 s
62℃退火	30 s	2~4步30 Cycles
72℃延伸	45 s
72℃延伸	10 min
4℃保存	Forever

表2 BMPR-IB基因PCR反应程序

Tab.2 PCR reaction program for BMPR-IB gene

温度 Temperature	时间 Time	循环次数 Number of cycles
94℃预变性	5 min
94℃变性	30 s
60℃退火	30 s	2~4步30 Cycles
72℃延伸	1 min
72℃延伸	5~10 min
4℃保存	Forever

图1 多湖杂交后代DNA提取结果

Fig.1 Results of DNA testing of the progeny of Duo and Hu crossbred sheep

图2 COIL基因在多湖杂交F1代中的ARMS-PCR检测结果

Fig.2 ARMS-PCR results of COIL gene in Duohu crossbred sheep F1

图3 COIL基因在多湖杂交F2代中的ARMS-PCR检测结果

Fig.3 ARMS-PCR results of COIL gene in Duohu crossbred sheep F2

图4 COIL基因在多湖杂交F3代中的ARMS-PCR检测结果

Fig.4 ARMS-PCR results of COIL gene in Duohu crossbred sheep F3

图5 BMPR-IB基因PCR 检测结果

Fig.5 BMPR-IB gene PCR test results

图6 BMPR-IB基因在多湖杂交F1代中的PCR-RFLP检测结果

Fig.6 PCR-RFLP results of BMPR-IB gene in Duohu crossbred sheep F1

图7 BMPR-IB基因在多湖杂交F2代中的PCR-RFLP检测结果

Fig.7 PCR-RFLP results of BMPR-IB gene in Duohu crossbred sheep F2

图8 BMPR-IB基因在多湖杂交F3代中的PCR-RFLP检测结果

Fig.8 PCR-RFLP results of BMPR-IB gene in Duohu crossbred sheep F3

表3 COIL基因在多湖杂交后代中的基因型频率、等位基因频率

Tab.3 Genotype frequency and allele frequency of COIL gene in Duohu crossbred sheep

品种 Breed	基因型频率 Genotype frequency			P值 P value	等位基因频率 Gene frequency
品种 Breed	CC	CG	GG	P值 P value	C	G
多湖杂交F₁代 (228只)	0.070 (26)	0.667 (247)	0.263 (97)	<0.05	0.404	0.596
多湖杂交F₂代 (254只)	0.055 (23)	0.657 (269)	0.287 (118)	<0.05	0.384	0.616
多湖杂交F₃代 (252只)	0.044 (24)	0.639 (345)	0317 (171)	<0.05	0.363	0.637

表4 BMPR-IB基因在多湖杂交后代中的基因型频率、等位基因频率

Tab.4 Genotype frequency and allele frequency of BMPR-IB gene in Duohu crossbred sheep

品种 Breed	基因型频率 Genotype frequency			P值 P value	等位基因频率 Gene frequency
品种 Breed	++	B+	BB	P值 P value	+	B
多湖杂交F₁代 (228只)	0.297 (110)	0.689 (255)	0.014 (5)	<0.05	0.642	0.358
多湖杂交F₂代 (254只)	0.288 (118)	0.698 (286)	0.014 (6)	<0.05	0.637	0.363
多湖杂交F₃代 (252只)	0.276 (149)	0.713 (385)	0.011 (6)	<0.05	0.632	0.368

表5 COIL基因在多湖杂交后代中相同代数、不同基因型与产羔数关联性

Tab.5 Analysis of the association between the same number of generations, different genotypes and the number of lambs produced by the COIL gene in the progeny of Duohu crossbred sheep

品种 Breed	基因型 Genotype
品种 Breed	CC (Mean±SD)	CG (Mean±SD)	GG (Mean±SD)
多湖杂交F₁代 (228只)	1.38± 0.500^C	1.78± 0.418^B	2.00± 0.260^A
多湖杂交F₂代 (254只)	1.36± 0.497^C	1.89± 0.311^B	2.04± 0.351^A
多湖杂交F₃代 (252只)	1.45± 0.522^B	2.02± 0.284^Ab	2.13± 0.432^Aa

表6 BMPR-IB基因在多湖杂交后代中相同代数、不同基因型与产羔数关联性

Tab.6 Analysis of the association between the same number of generations, different genotypes and the number of lambs produced by the BMPR-IB gene in the progeny of Duohu crossbred sheep

品种 Breed	基因型 Genotype
品种 Breed	++ (Mean±SD)	B+ (Mean±SD)	BB (Mean±SD)
多湖杂交F₁代 (228只)	1.57± 0.499^B	1.90± 0.323^A	2.33± 0.577^A
多湖杂交F₂代 (254只)	1.68± 0.468^C	1.98± 0.249^B	2.50± 0.577^A
多湖杂交F₃代 (252只)	1.80± 0.405^C	2.11± 0.315^B	2.67± 0.577^A

表7 COIL基因在多湖杂交后代中不同代数、相同基因型与产羔数关联性

Tab.7 Analysis of the association between different generations of the COIL gene, the same genotype and the number of lambs produced in the progeny of Duohu crossbred sheep

基因型 Genotype	品种Breed
基因型 Genotype	多湖杂交F₁代 (Mean±SD)	多湖杂交F₂代 (Mean±SD)	多湖杂交F₃代 (Mean±SD)
CC型 (41只)	1.38± 0.500	1.36± 0.497	1.45± 0.522
CG型 (480只)	1.78± 0.418^C	1.89± 0.311^B	2.02± 0.284^A
GG型 (213只)	2.00± 0.260^b	2.04± 0.351	2.13± 0.432^a

表8 BMPR-IB基因在多湖杂交后代中不同代数、相同基因型与产羔数关联性

Tab.8 Analysis of the association between different generations of the BMPR-IB gene, the same genotype and the number of lambs produced in the progeny of Duohu crossbred sheep

基因型 Genotype	品种Breed
基因型 Genotype	多湖杂交F₁代 (Mean±SD)	多湖杂交F₂代 (Mean±SD)	多湖杂交F₃代 (Mean±SD)
++型 (209只)	1.57± 0.499^B	1.68± 0.468 ^A	1.80± 0.405 ^A
B+型 (514只)	1.90± 0.323^C	1.98± 0.249^B	2.11± 0.315^A
BB型 (10只)	2.33± 0.577	2.5± 0.577	2.67± 0.577

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