新疆农业科学 ›› 2024, Vol. 61 ›› Issue (4): 1021-1028.DOI: 10.6048/j.issn.1001-4330.2024.04.027
• 植物保护·微生物·农业装备工程与机械化 • 上一篇 下一篇
孙建1(), 李雪2, 楚敏1, 顾美英1, 艾尼江·尔斯满1, 朱静1, 何齐2, 谭慧林3, 张志东1,2(
)
收稿日期:
2023-08-07
出版日期:
2024-04-20
发布日期:
2024-05-31
通信作者:
张志东(1977-),男,新疆乌鲁木齐人,研究员,博士,硕士生/博士生导师,研究方向为特殊环境微生物及益生菌资源,(E-mail)zhangzheedong@qq.com作者简介:
孙建(1977-),男,新疆乌鲁木齐人,副研究员,研究方向为特殊环境微生物及益生菌资源,(E-mail)276255465@qq.com
基金资助:
SUN Jian1(), LI Xue2, CHU Min1, GU Meiying1, Ainijiang Ersiman1, ZHU Jin1, HE Qi2, TAN Huilin3, ZHANG Zhidong1,2(
)
Received:
2023-08-07
Published:
2024-04-20
Online:
2024-05-31
Correspondence author:
ZHANG Zhidong(1977-),male, from Urumqi,Xinjiang,master, researcher, research direction: Special environmental microorganisms and food Microbiology,(E-mail)zhangzheedong@qq.comSupported by:
摘要:
【目的】挖掘和获得原驼乳中的乳酸菌资源。【方法】采集新疆乌鲁木齐县近效南山周边原驼乳样品,采用平板稀释法分离菌株,观察比对16S rRNA基因序列测序和菌株形态学特性,分析菌株分类学地位,并检测菌株溶血性和有害代谢物质等食用安全性。【结果】获得29株乳酸菌,经鉴定其归属于Leuconostoc、Lentilactobacillus两个属的4个种;菌株均可耐受0.5%胆盐,5%NaCl,且具有较好耐高温、牛奶胨化、抗氧化和抑制细菌等特性;所有菌株均无溶血现象,不产生硝基还原酶;除T12外,均利用氨基酸不产生物胺。【结论】从原驼乳中获得的乳酸菌具有良好的发酵特性和食品安全性。
中图分类号:
孙建, 李雪, 楚敏, 顾美英, 艾尼江·尔斯满, 朱静, 何齐, 谭慧林, 张志东. 原驼乳中乳酸菌的分离筛选及特性分析[J]. 新疆农业科学, 2024, 61(4): 1021-1028.
SUN Jian, LI Xue, CHU Min, GU Meiying, Ainijiang Ersiman, ZHU Jin, HE Qi, TAN Huilin, ZHANG Zhidong. Screening,identification and characteristics of Lactic acid bacteria from raw camel milk[J]. Xinjiang Agricultural Sciences, 2024, 61(4): 1021-1028.
菌株 Bacterial strain | 温度 Temperature(℃) | NaCl (%) | pH | 胆盐 Bile salt(%) | 牛奶胨化 Milk peptone | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
20 | 45 | 3 | 5 | 7 | 3 | 4 | 0.5 | 胨化 Peptoniz- ation | 产香 Aroma production | |||||
T6 | w | + | + | + | - | - | w | ++ | + | + | ||||
T7 | w | + | + | + | - | - | w | ++ | + | + | ||||
T12 | + | + | + | + | - | - | w | ++ | + | ++ | ||||
T16 | w | + | + | + | - | - | w | ++ | + | + |
表1 部分菌株生长
Tab.1 The growth of representative strains under different conditions
菌株 Bacterial strain | 温度 Temperature(℃) | NaCl (%) | pH | 胆盐 Bile salt(%) | 牛奶胨化 Milk peptone | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
20 | 45 | 3 | 5 | 7 | 3 | 4 | 0.5 | 胨化 Peptoniz- ation | 产香 Aroma production | |||||
T6 | w | + | + | + | - | - | w | ++ | + | + | ||||
T7 | w | + | + | + | - | - | w | ++ | + | + | ||||
T12 | + | + | + | + | - | - | w | ++ | + | ++ | ||||
T16 | w | + | + | + | - | - | w | ++ | + | + |
菌株 Bacterial strain | 功能酶Functional enzyme | ||||
---|---|---|---|---|---|
蛋白酶 Protease | 淀粉酶 Amylase | 果胶酶 Pectinase | 酯化酶 Esterase | 纤维素酶 Cellulase | |
T6 | + | - | - | - | - |
T7 | ++ | - | - | - | + |
T12 | - | - | - | - | - |
T16 | + | - | - | - | - |
表2 菌株功能酶活性
Tab.2 Functional enzyme activity of strains
菌株 Bacterial strain | 功能酶Functional enzyme | ||||
---|---|---|---|---|---|
蛋白酶 Protease | 淀粉酶 Amylase | 果胶酶 Pectinase | 酯化酶 Esterase | 纤维素酶 Cellulase | |
T6 | + | - | - | - | - |
T7 | ++ | - | - | - | + |
T12 | - | - | - | - | - |
T16 | + | - | - | - | - |
菌株 Bacterial strain | 指示菌Indicator bacteria | |||||
---|---|---|---|---|---|---|
大肠埃希菌 Escherichia coli | 枯草芽孢杆菌 Bacillus subtilis | 金黄色葡萄球菌 Staphylococcus aureus | 扩展青霉 Expanded cyanobacteria | 黑曲霉 Aspergillus niger | 白色念珠菌 Candida albicans | |
T6 | +++ | + | +++ | - | - | - |
T7 | - | - | + | - | - | - |
T12 | ++ | + | +++ | - | - | - |
T16 | +++ | - | ++ | - | - | - |
表3 菌株的抑菌特性
Tab.3 Inhibition character of tested strains against indicator strains
菌株 Bacterial strain | 指示菌Indicator bacteria | |||||
---|---|---|---|---|---|---|
大肠埃希菌 Escherichia coli | 枯草芽孢杆菌 Bacillus subtilis | 金黄色葡萄球菌 Staphylococcus aureus | 扩展青霉 Expanded cyanobacteria | 黑曲霉 Aspergillus niger | 白色念珠菌 Candida albicans | |
T6 | +++ | + | +++ | - | - | - |
T7 | - | - | + | - | - | - |
T12 | ++ | + | +++ | - | - | - |
T16 | +++ | - | ++ | - | - | - |
图3 菌株的抑菌活性 注:A:大肠埃希菌;B:枯草芽孢杆菌;C:金黄色葡萄球菌
Fig.3 Inhibition of the tested strains against indicator strains Note:A:Escherichia coli;B:Bacillas subtilis;C:Staphylococcus aureus
抗生素种类 Types of antibi otics | 受试菌株 Test strain | |||
---|---|---|---|---|
T6 | T7 | T12 | T16 | |
青霉素PEN | +++ | + | ++ | +++ |
红霉素ERY | +++ | ++ | + | +++ |
四环素TET | - | - | ++ | +++ |
链霉素STR | - | - | + | + |
庆大霉素GEN | + | + | - | +++ |
卡那霉素KAN | ++ | + | ++ | ++ |
诺氟沙星NOR | - | - | - | - |
氨苄西林AMP | + | - | - | + |
利福平RIF | +++ | ++ | +++ | +++ |
万古霉素VAN | - | - | + | - |
表4 菌株抗生素敏感性检测
Tab.4 Antibiotic sensitivity of the strains
抗生素种类 Types of antibi otics | 受试菌株 Test strain | |||
---|---|---|---|---|
T6 | T7 | T12 | T16 | |
青霉素PEN | +++ | + | ++ | +++ |
红霉素ERY | +++ | ++ | + | +++ |
四环素TET | - | - | ++ | +++ |
链霉素STR | - | - | + | + |
庆大霉素GEN | + | + | - | +++ |
卡那霉素KAN | ++ | + | ++ | ++ |
诺氟沙星NOR | - | - | - | - |
氨苄西林AMP | + | - | - | + |
利福平RIF | +++ | ++ | +++ | +++ |
万古霉素VAN | - | - | + | - |
菌株 Bacterial strain | 生物胺Biogenic amine | 吲哚 Indole | 溶血性 Hemolysis | 硝基还原酶 Nitroreductase | ||||
---|---|---|---|---|---|---|---|---|
精胺 Spermine | 酪胺 Tyramine | 色胺 Tryptamine | 尸胺 Cadaverine | 组胺 Histamine | ||||
T6 | - | - | - | - | - | - | - | - |
T7 | - | - | - | - | - | - | - | - |
T12 | - | + | + | - | + | + | - | - |
T16 | - | - | - | - | - | - | - | - |
*E.coli | (CK: -) | + | + | + |
表5 菌株的有害代谢物质检测
Tab.5 The detection results of harmful metabolites of strains
菌株 Bacterial strain | 生物胺Biogenic amine | 吲哚 Indole | 溶血性 Hemolysis | 硝基还原酶 Nitroreductase | ||||
---|---|---|---|---|---|---|---|---|
精胺 Spermine | 酪胺 Tyramine | 色胺 Tryptamine | 尸胺 Cadaverine | 组胺 Histamine | ||||
T6 | - | - | - | - | - | - | - | - |
T7 | - | - | - | - | - | - | - | - |
T12 | - | + | + | - | + | + | - | - |
T16 | - | - | - | - | - | - | - | - |
*E.coli | (CK: -) | + | + | + |
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摘要 164
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