新疆农业科学 ›› 2023, Vol. 60 ›› Issue (9): 2323-2330.DOI: 10.6048/j.issn.1001-4330.2023.09.029
何齐1(), 冯倩1, 李雪1, 易鸳鸯2, 顾美英2, 朱静2, 孙建2, 张志东1,2(
)
收稿日期:
2022-12-26
出版日期:
2023-09-20
发布日期:
2023-09-19
通信作者:
张志东(1977-),男,新疆乌鲁木齐人,研究员,博士,硕士生导师,研究方向为特殊环境微生物及益生菌资源挖掘,(E-mail)zhangzheedong@qq.com作者简介:
何齐(1997-),男,安徽人,硕士研究生,研究方向为食品科学与食品安全, (E-mail)heqi9711@163.com
基金资助:
HE Qi1(), FENG Qian1, LI Xue1, YI Yuanyang2, GU Meiying2, ZHU Jin2, SUN Jian2, ZHANG Zhidong1,2(
)
Received:
2022-12-26
Published:
2023-09-20
Online:
2023-09-19
Supported by:
摘要:
【目的】挖掘和利用赛里木酸奶中的乳酸菌资源。【方法】采集12份阿克苏地区赛里木镇周边酸奶样品,采用平板稀释法进行菌株分离,通过16S rRNA基因序列测序和菌株形态学观察和明确菌株分类学地位,检测菌株溶血性和有害代谢物质等。【结果】在12种赛里木酸奶样品中筛选得到4种乳酸菌,分属于Eremococcus、Leuconostoc、Lentilactobacillus、 Streptococcus 4个属下的4个种,试验菌株可耐受0.5%胆盐,0.5%NaCl,且具有较好耐高温、牛奶胨化和抑制细菌等特性,并展现出良好的自聚合能力;所有菌株均无溶血现象;除H5-4外利用氨基酸均不产生物胺,并不产生硝基还原酶。【结论】从赛里木酸奶中分离获得的乳酸菌具有良好的发酵特性和食品安全性。
中图分类号:
何齐, 冯倩, 李雪, 易鸳鸯, 顾美英, 朱静, 孙建, 张志东. 赛里木酸奶中乳酸菌的分离鉴定及特性分析[J]. 新疆农业科学, 2023, 60(9): 2323-2330.
HE Qi, FENG Qian, LI Xue, YI Yuanyang, GU Meiying, ZHU Jin, SUN Jian, ZHANG Zhidong. Screening, identification and characteristics of Lactic acid bacteria from the sayram ketteki[J]. Xinjiang Agricultural Sciences, 2023, 60(9): 2323-2330.
序号 Serial number | 编号 Code numble | 采集地点 Sampling position | 备注 Remark |
---|---|---|---|
1 | H1 | 拜城县黑英山乡亚吐尔村4组 | 自产 |
2 | H2 | 拜城县黑英山乡8村1组 | 半商品化 |
3 | H3 | 拜城县黑英山乡8村2组 | 自产 |
4 | H4 | 拜城县黑英山乡明布拉克村大组 | 自产 |
5 | H5 | 拜城县黑英山乡1村4组 | 自产 |
6 | D1 | 拜城县赛里木酸奶厂 | 商品化 |
7 | M1 | 赛里木镇2村2大队 | 自产 |
8 | K1 | 拜城县克孜尔乡 | 自产 |
9 | S1 | 拜城县赛里木酸奶厂 | 商品化 |
10 | S4 | 赛里木镇托喀其买里村 | 半商品化 |
11 | S5 | 赛里木镇6村1组 | 自产 |
12 | S6 | 赛里木镇2村2大队 | 自产 |
表1 样品及来源
Tab.1 The samples and samples origin
序号 Serial number | 编号 Code numble | 采集地点 Sampling position | 备注 Remark |
---|---|---|---|
1 | H1 | 拜城县黑英山乡亚吐尔村4组 | 自产 |
2 | H2 | 拜城县黑英山乡8村1组 | 半商品化 |
3 | H3 | 拜城县黑英山乡8村2组 | 自产 |
4 | H4 | 拜城县黑英山乡明布拉克村大组 | 自产 |
5 | H5 | 拜城县黑英山乡1村4组 | 自产 |
6 | D1 | 拜城县赛里木酸奶厂 | 商品化 |
7 | M1 | 赛里木镇2村2大队 | 自产 |
8 | K1 | 拜城县克孜尔乡 | 自产 |
9 | S1 | 拜城县赛里木酸奶厂 | 商品化 |
10 | S4 | 赛里木镇托喀其买里村 | 半商品化 |
11 | S5 | 赛里木镇6村1组 | 自产 |
12 | S6 | 赛里木镇2村2大队 | 自产 |
菌株 Strains | 温度 Temperature (℃) | NaCl(%) | pH | 胆盐 Bile salt (%) | 牛奶胨化 Milk peptonization | |||||
---|---|---|---|---|---|---|---|---|---|---|
20 | 45 | 3 | 5 | 7 | 3 | 4 | 0.5 | 胨化 Peptization | 产香 Fragrance | |
S5-1 | + | + | + | + | + | - | w | ++ | + | + |
D1-3 | + | + | + | + | - | - | w | ++ | + | + |
H4-2 | + | + | + | + | - | - | w | ++ | + | ++ |
H5-4 | + | + | + | + | - | - | w | ++ | + | + |
表2 菌株的生长情况
Tab.2 The growth of strains under different conditions
菌株 Strains | 温度 Temperature (℃) | NaCl(%) | pH | 胆盐 Bile salt (%) | 牛奶胨化 Milk peptonization | |||||
---|---|---|---|---|---|---|---|---|---|---|
20 | 45 | 3 | 5 | 7 | 3 | 4 | 0.5 | 胨化 Peptization | 产香 Fragrance | |
S5-1 | + | + | + | + | + | - | w | ++ | + | + |
D1-3 | + | + | + | + | - | - | w | ++ | + | + |
H4-2 | + | + | + | + | - | - | w | ++ | + | ++ |
H5-4 | + | + | + | + | - | - | w | ++ | + | + |
菌株 Strains | 功能酶 Functional enzymes | ||||
---|---|---|---|---|---|
蛋白酶 Protease | 淀粉酶 Amylase | 果胶酶 Pectase | 酯化酶 Esterase | 纤维素酶 Cellulase | |
S5-1 | + | - | - | - | - |
D1-3 | + | - | - | - | - |
H4-2 | + | - | - | - | - |
H5-4 | + | - | - | - | - |
表3 菌株功能酶活性
Tab.3 Functional enzyme activity of strains
菌株 Strains | 功能酶 Functional enzymes | ||||
---|---|---|---|---|---|
蛋白酶 Protease | 淀粉酶 Amylase | 果胶酶 Pectase | 酯化酶 Esterase | 纤维素酶 Cellulase | |
S5-1 | + | - | - | - | - |
D1-3 | + | - | - | - | - |
H4-2 | + | - | - | - | - |
H5-4 | + | - | - | - | - |
菌株 Strains | 病原指示菌 Pathogen indicator | |||||
---|---|---|---|---|---|---|
大肠埃希菌 E.coli | 枯草芽孢杆菌 B.subtilis | 金黄色葡萄球菌 S.aureus | 青霉菌 Penicillium | 黑曲霉 Asp.niger | 白色念珠菌 C.albicans | |
S5-1 | ++ | - | + | - | - | - |
D1-3 | +++ | + | ++ | - | - | - |
H4-2 | +++ | ++ | +++ | - | - | + |
H5-4 | + | + | + | - | - | - |
表4 菌株的抑菌活性
Tab.4 Antibacterial activity of strains
菌株 Strains | 病原指示菌 Pathogen indicator | |||||
---|---|---|---|---|---|---|
大肠埃希菌 E.coli | 枯草芽孢杆菌 B.subtilis | 金黄色葡萄球菌 S.aureus | 青霉菌 Penicillium | 黑曲霉 Asp.niger | 白色念珠菌 C.albicans | |
S5-1 | ++ | - | + | - | - | - |
D1-3 | +++ | + | ++ | - | - | - |
H4-2 | +++ | ++ | +++ | - | - | + |
H5-4 | + | + | + | - | - | - |
菌株 Strains | 生物胺 Biogenic amines | 吲哚 Indoles | 溶血性 hemolytic | 硝基还原酶 Nitroreductase | ||||
---|---|---|---|---|---|---|---|---|
精胺 Spermine | 酪胺 Tyramine | 色胺 Tryptamine | 尸胺 Cadaverine | 组胺 Histamine | ||||
S5-1 | - | - | - | - | - | - | - | - |
D1-3 | - | - | - | - | - | - | - | - |
H4-2 | - | - | - | - | - | - | - | - |
H5-4 | - | - | + | - | - | - | - | + |
*E.coli | (CK: -) | + | + | + |
表5 菌株的有害代谢物质检测
Tab.5 The detection results of harmful metabolites of strains
菌株 Strains | 生物胺 Biogenic amines | 吲哚 Indoles | 溶血性 hemolytic | 硝基还原酶 Nitroreductase | ||||
---|---|---|---|---|---|---|---|---|
精胺 Spermine | 酪胺 Tyramine | 色胺 Tryptamine | 尸胺 Cadaverine | 组胺 Histamine | ||||
S5-1 | - | - | - | - | - | - | - | - |
D1-3 | - | - | - | - | - | - | - | - |
H4-2 | - | - | - | - | - | - | - | - |
H5-4 | - | - | + | - | - | - | - | + |
*E.coli | (CK: -) | + | + | + |
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