新疆农业科学 ›› 2019, Vol. 56 ›› Issue (6): 1052-1062.DOI: 10.6048/j.issn.1001-4330.2019.06.008
王振东, 鲁晓燕, 涂文文, 王晓丽, 何晨晨
出版日期:
2019-06-20
发布日期:
2019-08-08
作者简介:
王振东(1995-),男,硕士研究生,研究方向为果树学,(E-mail)1753669743@qq.com
基金资助:
WANG Zhen-Dong, LU Xiao-Yan, TU Wen-Wen, HE Chen-Chen
Published:
2019-06-20
Online:
2019-08-08
Supported by:
摘要: 【目的】研究CaCl2缓解酸枣盐胁迫的分子机制。通过转录组测序分析,为研究CaCl2缓解酸枣盐害分子机制提供参考。【方法】研究以水培酸枣幼苗为材料,150 mmol/L NaCl和150 mmol/L NaCl + 10 mmol/L CaCl2分别处理6 h,利用高通量测序技术对酸枣幼苗叶片和根系进行了转录组测序与组装,对获得的所有Unigene在GO、KEGG数据库中进行功能注释。【结果】 与对照组相比,在外源CaCl2处理下酸枣幼苗叶片上调和下调的差异表达基因分别7 365个和1 247个,根系分别有762个和4 861个。【结论】GO数据库比对,酸枣幼苗叶片富集了43个GO条件,根系富集了42个GO条件,叶片和根系均是催化活性被富集到差异基因最多;KEGG数据库比对,差异基因主要涉及植物病原互作、MAPK信号通路-植物、植物激素信号转导、苯丙醇生物合成、戊糖和葡萄糖醛酸相互转化等。
王振东, 鲁晓燕, 涂文文, 王晓丽, 何晨晨. 外源CaCl2缓解NaCl胁迫下酸枣幼苗叶和根转录组测序分析[J]. 新疆农业科学, 2019, 56(6): 1052-1062.
WANG Zhen-Dong, LU Xiao-Yan, TU Wen-Wen, HE Chen-Chen. Transcriptome Sequencing Analysis of Leaf and Root of Sour Jujube Seedlings under NaCl Stress Alleviated by Exogenous CaCl2[J]. Xinjiang Agricultural Sciences, 2019, 56(6): 1052-1062.
[1] | 杨少辉, 季静, 王罡,等. 盐胁迫对植物影响的研究进展[J]. 分子植物育种, 2006, 4(S1):139-142.YANG Shao-hui, JI Jing, WANG Gang, et al. (2006). Effects of Salt Stress on Plants [J]. Molecular Plant Breeding, 4(S1):139-142. (in Chinese) |
[2] | Deinlein, U. , Stephan, A. B. , Horie, T. , Luo, W. , & Schroeder, J. I. (2014). Plant salt-tolerance mechanisms. Trends in Plant Science, 19(6):371-379. |
[3] | Liang, W., Ma, X., Wan, P., & Liu, L. (2017). Plant salt-tolerance mechanism: a review. Biochemical and Biophysical Research Communications, 495(1): 286-291. |
[4] | 章文华, 陈亚华, 刘友良. 钙在植物细胞盐胁迫信号转导中的作用[J]. 植物生理学通讯, 2000, 36(2):146-153.ZHANG Wen-hua, CHEN Ya-hua, LIU You-liang. (2000). Calcium Action in Signal Transduction in Plant Cells Under Salt Stress [J]. Plant Physiology Communications, 36(2):146-153. (in Chinese) |
[5] | 戴高兴, 彭克勤, 皮灿辉. 钙对植物耐盐性的影响[J]. 中国农学通报, 2003, 19(3):97.DAI Gao-xing, PENG Ke-qing, PI Can-hui. (2003). The Effects of Calcium on Salt-tolerance in Plan [J]. Chinese Agricultural Science Bulletin, 19(3):97. (in Chinese) |
[6] | 刘孟军, 诚静容. 枣和酸枣的分类学研究[J]. 河北农业大学学报, 1994,(4):1-10.LIU Meng-jun, CHEN Jin-rong. (1994). A Taxonomic Study on Chinese Jujube and Wild Jujube [J]. Journal of Hebei Agricultural University, (4):1-10. (in Chinese) |
[7] | 王向红, 崔同, 刘孟军,等. 不同品种枣的营养成分分析[J]. 营养学报, 2002, 24(2):206-208.WANG Xiang-hong, CUI Tong, LIU Meng-jun, et al. (2002). Analysis of Nutritional Composition of Different Chinese Jujubes [J]. Acta Nutrimenta Sinica, 24(2):206-208. (in Chinese) |
[8] | 刘孟军,汪民.中国枣种质资源[M]. 北京:中国林业出版社,2009:41-45.LIU Meng-jun, WANG Ming. (2009). Germplasm resources of Chinese jujube [M]. Beijing:China Forestry Publishing House:41-45. (in Chinese) |
[9] | Dong, Z. C., & Chen, Y. (2013). Transcriptomics: advances and approaches. SCIENCE CHINA Life Sciences, 56(10):960-967. |
[10] | Ansorge, W. J. (2009). Next-generation DNA sequencing techniques. N Biotechnol, 25(4):195-203. |
[11] | 杨怡帆, 吕新民, 鲁晓燕,等. CaCl2缓解酸枣幼苗NaCl胁迫的主成分分析[J]. 果树学报, 2016,(8):959-968.YANG Yi-fan, L Xin-ming, LU Xiao-yang, et al. (2016) Principal component analysis of the alleviating effects of CaCl2 on NaCl stress in Sour jujube (Ziziphus acidojujuba) seedlings[J]. Journal of Fruit Science, (8):959-968. (in Chinese) |
[12] | 吕新民, 杨怡帆, 鲁晓燕,等. CaCl2对NaCl胁迫下酸枣苗AsA-GSH循环的影响[J]. 园艺学报, 2017, 44(5):953-962.L Xin-ming, YANG Yi-fan, LU Xiao-yang, et al. (2017). Effects of CaCl2 on the AsA-GSH Cycle of Sour jujube Seedlings under NaCl Stress [J]. Acta Horticulturae Sinica, 44(5):953-962. (in Chinese) |
[13] | 王晓丽, 鲁晓燕, 涂文文, 等. 外源CaCl2对NaCl胁迫下酸枣幼苗氮代谢的影响[J]. 西北植物学报, 2018, 38(9):1 683-1 691.WANG Xiao-li, LU Xiao-yang, TU Wen-wen, et al. (2018). Effect of Exogenous CaCl2 on the Nitrogen Metabolism of Sour jujube Seedling under NaCl Stress [J]. Acta Botanica Boreali-Occidentalia Sinica,38(9):1,683-1,691. (in Chinese) |
[14] | Wang, L., Feng, Z. , Wang, X. , Wang, X. , & Zhang, X. (2010). DEGseq: an R package for identifying differentially expressed genes from RNA-seq data. Bioinformatics, 26(1):136-138. |
[15] | Ozsolak, F., & Milos, P. M. (2010). RNA sequencing: advances, challenges and opportunities. Nature Reviews Genetics, 12(2):87-98. |
[16] | 贾昌路, 张瑶, 朱玲, 等. 转录组测序技术在生物测序中的应用研究进展[J]. 分子植物育种, 2015, 13(10):2 388-2 394.JIA Chang-lu, ZHANG Yao, ZHU Ling, et al. (2015). Application Progress of Transcriptome Sequencing Technology in Biological Sequencing [J]. Molecular Plant Breeding, 13(10):2,388-2,394. (in Chinese) |
[17] | 薛辉,曹尚银,李好先,等.转录组技术在果树研究中的应用[J].江西农业学报,2015,27(5):16-21.XUE Hui, CAO Shang-yin, LI Hao-xian, et al. (2015). Application of Transcriptome Technology in Fruit Tree Research [J]. Acta Agriculturae Jiangxi, 27(5):16-21. (in Chinese) |
[18] | 吉福桑,李元元,唐露,等.香蕉叶片响应盐胁迫转录组分析[J].分子植物育种,2017,15(3):875-882.JI Fu-sang, LI Yuan-yuan, TANG Lu, et al. (2017). Analysis of Banana Leaves Responses Salt Stress of Transcriptome [J]. Molecular Plant Breeding, 15(3):875-882. (in Chinese) |
[19] | Anuradha, U. , Tulsi, G. , Kumar, U. A. , Satisha, J. , Shinde, M. P. , & Kadoo, N. Y. , et al. (2018). Global transcriptome analysis of grapevine (vitis vinifera, l.) leaves under salt stress reveals differential response at early and late stages of stress in table grape cv. Thompson seedless. Plant Physiology and Biochemistry, 129:168-179. |
[20] | Yuanyuan Xu, Xiaogang Li, Jing Lin, Zhonghua Wang, Qingsong Yang, Youhong Chang. (2015). Transcriptome sequencing and analysis of major genes involved in calcium signaling pathways in pear plants (pyrus calleryana decne.). BMC Genomics, 16(1): 738. |
[21] | 宋秀芬,洪剑明.植物细胞中钙信号的时空多样性与信号转导[J]. 植物学通报, 2001, 18(4):436-444.SONG Xiu-feng, HONG Jian-ming. (2001). Spatio_temporal Varieties of Calcium Signals and Signals Transduction in Plant Cell [J]. Chinese Bulletin of Botany, 18(4): 436-444. (in Chinese) |
[22] | Berridge, M. J. (1997). Elementary and global aspects of calcium signalling. Journal of Experimental Biology, 200(2):315-319. |
[23] | 李洁.植物转录因子与基因调控[J].生物学通报,2004, 39(3):9-11.LI Jie. (2004). Plant transcription factors and gene regulation [J]. Bulletin of Biology, 39(3):9-11. (in Chinese) |
[24] | 郭仰东, 张磊, 李双桃,等. 蔬菜作物应答非生物逆境胁迫的分子生物学研究进展[J]. 中国农业科学, 2018,51(6):1 167-1 181.GUO Yang-dong, ZHANG Lei, LI Shuang-tao, et al. (2018). Advances in molecular biology of vegetable crops in response to abiotic stress [J]. Scientia Agricultura Sinica, 51(6):1,167-1,181. (in Chinese) |
[25] | Batistic, O. , Sorek, N. , Schultke, S. , Yalovsky, S. , & Kudla, J. (2008). Dual fatty acyl modification determines the localization and plasma membrane targeting of cbl/cipk Ca2+ signaling complexes in arabidopsis. THE PLANT CELL ONLINE, 20(5):1,346-1,362. |
[26] | Jingjing, M., Manik, S., Sujuan, S., Jiangtao, C., Yirong, J., & Qian, W., et al. (2016). Mechanisms and physiological roles of the cbl-cipk networking system in arabidopsis thaliana. Genes,7(9): 62-75. |
[27] | Yan, Y. , He, X. , Hu, W. , Liu, G. , Wang, P. , & He, C. , et al. (2018). Functional analysis of mecipk23 and mecbl1/9 in cassava defense response against xanthomonas axonopodis pv. manihotis. Plant Cell Reports: 1-14. |
[28] | 朱冬梅,贾媛,崔继哲,等.植物对盐胁迫应答的转录因子及其生物学特性[J]. 生物技术通报, 2010,(4):16-21.ZHU Dong-mei, JIA Yuan, CUI Ji-ze, et al. (2010). Plant Transcription Factors in Response to Salt Stress and Its Biological Characteristics [J]. Biotechnology Bulletin, (4):16-21. (in Chinese) |
[29] | Dubos, C. , Stracke, R. , Grotewold, E. , Weisshaar, B. , Martin, C. , & Lepiniec, L. (2010). Myb transcription factors in arabidopsis. Trends in Plant Science, 15(10):573-581. |
[30] | Martin, C. (1997). Myb transcription factors in plants. Trends Gene, 13(2): 67-73. |
[31] | Fang, Q., Jiang, T., Xu, L., Liu, H., Mao, H., & Wang, X., et al. (2017). A salt-stress-regulator from the poplar r2r3 myb family integrates the regulation of lateral root emergence and aba signaling to mediate salt stress tolerance in arabidopsis. Plant Physiology & Biochemistry, 114:100-110. |
[32] | Ting Wang, Takayuki Tohge, Alexander Ivakov, Bernd Mueller-Roeber, Alisdair R. Fernie, Marek Mutwil, Jos H.M. Schippers, and Staffan Persson. (2015). Salt-Related MYB1 (SRM1) Coordinates Abscisic Acid Biosynthesis and Signaling During Salt Stress in Arabidopsis. Plant Physiology, 169:1,027-1,041. |
[33] | 陈嘉贝,张芙蓉,黄丹枫,等.盐胁迫下两个甜瓜品种转录因子的转录组分析[J].植物生理学报,2014,50(2):150-158.CHEN Jia-bei, ZHANG Fu-rong, HUANG Dan-feng, et al. (2014). Transcriptome Analysis of Transcription Factors in Two Melon (Cucumis melo L.) Cultivars under Salt Stress [J]. Plant Physiology Journal, 50(2):150-158. (in Chinese) |
[34] | 秦圣豪,韩燕,崔凤,等.盐胁迫条件下花生应答转录因子鉴定与分析[J].山东农业科学,2018,50(6):41-45. QIN Sheng-hao, HAN Yan, CUI Feng, et al. (2018). Identification and Analysis of Responsive Transcription Factors under Salt Stress in Peanut [J]. Shandong Agricultural Sciences, 50(6):41-45. (in Chinese) |
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