咯菌腈和苯甲·嘧菌酯与硒复配对向日葵菌核病的抑制作用

doi:10.6048/j.issn.1001-4330.2025.05.020

新疆农业科学 ›› 2025, Vol. 62 ›› Issue (5): 1226-1233.DOI: 10.6048/j.issn.1001-4330.2025.05.020

咯菌腈和苯甲·嘧菌酯与硒复配对向日葵菌核病的抑制作用

胡欣¹, 马朝阳¹, 于博然¹, 张晨光¹, 王鹏², 赵思峰¹, 惠慧¹(), 张学坤¹()

1.石河子大学农学院/新疆绿洲农业病虫害治理与植保资源利用重点实验室,新疆石河子 832000
2.新疆农垦科学院作物研究所,新疆石河子 832000

收稿日期:2024-10-21 出版日期:2025-05-20 发布日期:2025-07-09
通信作者: 张学坤(1987-),男,河南民权人,副教授,博士,研究方向为植物病理学,(E-mail) Zhangxk2459@163.com
作者简介:惠慧(1988-),女,陕西蒲城人,副教授,博士,研究方向为植物病理学,(E-mail) xihui@shzu.edu.cn
基金资助:
新疆生产建设兵团财政科技计划项目(2021AB011);新疆生产建设兵团中青年科技创新领军人才计划(2018CB022)

Study on the inhibitory effect of fluroxonil and benzosulfuryl ester combined with selenium on Sclerotinia stem rot in sunflower

HU Xin¹, MA Chaoyang¹, YU Boran¹, ZHANG Chenguang¹, WANG Peng², ZHAO Sifeng¹, XI Hui¹(), ZHANG Xuekun¹()

1. Key Laboratory for Oasis Agricultural Pest Management and Plant Protection Resource Utilization / Agricultural College of Shihezi University, Shihezi Xinjiang 832003, China
2. Crop Research Institute, Xinjiang Academy of Agricultural Reclamation Sciences, Shihezi Xinjiang 832000, China

Received:2024-10-21 Published:2025-05-20 Online:2025-07-09
About author:XI Hui (1988- ), female, from Pucheng, Shannxi, associate professor,Ph.D., research direction: plant pathology, (E-mail) xihui@shzu.edu.cn
Supported by:
Financial Technology Program Project of XPCC(2021AB011);Young and Middle-Aged Scientific and Technological Innovation Leading Talents Project of XPCC(2018CB022)

摘要/Abstract

摘要：

【目的】筛选并获得杀菌剂与硒的复配制剂,以降低杀菌剂用量并提升其对核盘菌(Sclerotinia sclerotiorum)所致向日葵菌核病的防治效果,为新疆向日葵菌核病的防治提供理论依据。【方法】咯菌腈、苯甲·嘧菌酯分别与硒复配后,测定其对向日葵核盘菌菌丝生长和菌丝形态、草酸分泌量及菌核产生量的影响,并通过盆栽试验验证其对菌核病的防效。【结果】0.5 mg/L咯菌腈和1 mg/L苯甲·嘧菌酯分别与30 mg/L硒复配后,均显著抑制了核盘菌的菌丝生长和菌核产生,且菌丝的形态发生变异;咯菌腈能抑制核盘菌的草酸分泌量。此外,2种杀菌剂与硒复配后均在一定程度上提高了对菌核病的防病效果。【结论】0.5 mg/L咯菌腈、1 mg/L苯甲·嘧菌酯与30 mg/L硒复配与单剂相较,有效阻碍了核盘菌的正常生长发育,增强向日葵的保护作用。

关键词: 向日葵; 核盘菌; 杀菌剂; 硒; 联合毒力

Abstract:

【Objective】 Screened and selected fungicide-selenium co-formulations to reduce fungicide dosage while enhancing their control efficacy against sunflower sclerotinia caused by Sclerotinia sclerotiorum,thereby providing a novel theoretical foundation for managing this disease in Xinjlang.【Methods】 The effects of fluroxonil and benzalpyrimidin on mycelia growth, mycelia morphology, oxalic acid secretion and sclerotium production of S. sclerotiorum were measured after being combined with selenium separately, and their control efficacy on sunflower sclerotinia was verified by pot experiment.【Results】 The combination of 0.5 mg/L fluroxonil and 1 mg/L benzalpyrimidin with 30 mg/L selenium significantly inhibited the mycelium growth and the generation of sclerotium, and the mycelium morphology changed. The secretion of sclerotinic acid decreased significantly after the combination of fluroxonil and selenium. In addition, the combination of two fungicides and selenium could improve the prevention and control effect of Sclerotinia stem rot to a certain extent. 【Conclusion】 Compared with a single dose, the combination of 0.5mg/L fludioxonil, 1mg/L benzyl azoxystrobin and 30mg/L selenium effectively hinder the normal growth and development of S. sclerotiorum, and enhance the protective effect on sunflower.

Key words: sunflower; Sclerotinia sclerotiorum; fungicide; selenium; combined virulence

中图分类号:

S435.655

胡欣, 马朝阳, 于博然, 张晨光, 王鹏, 赵思峰, 惠慧, 张学坤. 咯菌腈和苯甲·嘧菌酯与硒复配对向日葵菌核病的抑制作用[J]. 新疆农业科学, 2025, 62(5): 1226-1233.

HU Xin, MA Chaoyang, YU Boran, ZHANG Chenguang, WANG Peng, ZHAO Sifeng, XI Hui, ZHANG Xuekun. Study on the inhibitory effect of fluroxonil and benzosulfuryl ester combined with selenium on Sclerotinia stem rot in sunflower[J]. Xinjiang Agricultural Sciences, 2025, 62(5): 1226-1233.

图/表 5

图1 不同处理下核盘菌菌丝生长速率的变化注:A: CK;B: 0.5 mg/L咯菌腈;C: 1 mg/L苯甲·嘧菌酯;D: 硒30 mg/L;E: 0.5 mg/L咯菌腈 + 30 mg/L硒;F: 1 mg/L苯甲·嘧菌酯+ 30 mg/L硒

Fig.1 Changes of different treatments on mycelial growth rate of S. sclerotiorum Notes:A: CK; B: 0.5 mg/L fludioxonil; C: 1 mg/L benzovindiflupyr·azoxystrobin; D: 30 mg/L selenium; E: 0.5 mg/L fludioxonil + 30 mg/L selenium; F: 1 mg/L benzovindiflupyr·azoxystrobin + 30 mg/L selenium

图2 不同处理下核盘菌菌丝形态变异的变化注:A: CK;B: 0.5 mg/L咯菌腈;C: 1 mg/L苯甲·嘧菌酯;D: 硒30 mg/L;E: 0.5 mg/L咯菌腈 + 30 mg/L硒;F: 1 mg/L苯甲·嘧菌酯+ 30 mg/L硒

Fig.2 Changes of different treatments on mycelial morphologic variation of S. sclerotiorum Notes:A: CK; B: 0.5 mg/L fludioxonil; C: 1 mg/L benzovindiflupyr·azoxystrobin; D: 30 mg/L selenium; E: 0.5 mg/L fludioxonil + 30 mg/L selenium; F: 1 mg/L benzovindiflupyr·azoxystrobin + 30 mg/L selenium

图3 草酸标准曲线(A)及不同处理下的核盘菌草酸分泌量(B)

Fig. 3 Standard curve of oxalic acid (A) and oxalic acid secretion of S. sclerotiorum under different treatments (B)

图4 不同处理下核盘菌菌核产量的变化注:A: CK;B: 0.5 mg/L咯菌腈;C: 1 mg/L苯甲·嘧菌酯;D: 硒30 mg/L;E: 0.5 mg/L咯菌腈 + 30 mg/L硒;F: 1 mg/L苯甲·嘧菌酯+ 30 mg/L硒

Fig. 4 Changes of different treatments on the sclerotium yield of S. sclerotiorum Notes:A: CK; B: 0.5 mg/L fludioxonil; C: 1 mg/L benzovindiflupyr·azoxystrobin; D: 30 mg/L selenium; E: 0.5 mg/L fludioxonil + 30 mg/L selenium; F: 1 mg/L benzovindiflupyr·azoxystrobin + 30 mg/L selenium

图5 不同处理对核盘菌的抑制效果注:A: CK;B: 0.5 mg/L咯菌腈;C: 1 mg/L苯甲·嘧菌酯;D: 硒30 mg/L;E: 0.5 mg/L咯菌腈 + 30 mg/L硒;F: 1 mg/L苯甲·嘧菌酯+ 30 mg/L硒

Fig. 5 Determination of inhibition effect of different treatments on S. sclerotiorum Notes:A: CK; B: 0.5 mg/L fludioxonil; C: 1 mg/L benzovindiflupyr·azoxystrobin; D: 30 mg/L selenium; E: 0.5 mg/L fludioxonil + 30 mg/L selenium; F: 1 mg/L benzovindiflupyr·azoxystrobin + 30 mg/L selenium

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咯菌腈和苯甲·嘧菌酯与硒复配对向日葵菌核病的抑制作用

Study on the inhibitory effect of fluroxonil and benzosulfuryl ester combined with selenium on Sclerotinia stem rot in sunflower

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