基于非靶向代谢组学分析两种酵母培养物的成分差异

doi:10.6048/j.issn.1001-4330.2024.05.020

新疆农业科学 ›› 2024, Vol. 61 ›› Issue (5): 1218-1226.DOI: 10.6048/j.issn.1001-4330.2024.05.020

基于非靶向代谢组学分析两种酵母培养物的成分差异

林青¹(), 时红玲¹, 秦新政¹, 李月¹, 王子涵¹, 高雁¹, 曾军¹, 王浩中², 娄恺¹(), 霍向东¹()

1.新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐 830091
2.新疆大学生命科学与技术学院,乌鲁木齐 830046

收稿日期:2023-10-15 出版日期:2024-05-20 发布日期:2024-07-09
通信作者: 娄恺(1968-),男,河南人,研究员,博士,硕士生导师,研究方向为微生物生态,(E-mail)loukai@tsinghua.org.cn;
霍向东(1974-),男,甘肃人,副研究员,博士,研究方向为微生物资源,(E-mail)xiangdonghuo@163.com
作者简介:林青(1985-),女,山东烟台人,副研究员,研究方向为微生物生态,(E-mail)qinglinxj@163.com
基金资助:
新疆维吾尔自治区自然科学基金重点项目(2023D01D11);新疆生产建设兵团重点领域科技攻关计划(2022AB013);新疆生产建设兵团重点领域科技攻关计划(2020AB013)

Analysis of the composition differences between two yeast cultures based on untargeted metabolomics

LIN Qing¹(), SHI Hongling¹, QIN Xinzheng¹, LI Yue¹, WANG Zihan¹, GAO Yan¹, ZENG Jun¹, WANG Haozhong², LOU Kai¹(), HUO Xiangdong¹()

1. Xinjiang Laboratory of Special Environmental Microbiology / Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
2. College of Life Science and Technology, Xinjiang University, Urumqi 830046, China

Received:2023-10-15 Published:2024-05-20 Online:2024-07-09
Correspondence author: LOU Kai(1968-),male,from Henan, researcher, master tutor, Ph.D.,research direction: microbial ecology,(E-mail)loukai@tsinghua.org.cn;
HUO Xiangdong (1974-), male, from Gansu, associate professor, Ph.D., research direction:microbial resources,(E-mail)xiangdonghuo@163.com
Supported by:
Key Program of Natural Science Foundation of Xinjiang Uygur Autonomous Region(2023D01D11);Key program of scientific and technological research plan of Xinjiang production and construction corps(2022AB013);Key program of scientific and technological research plan of Xinjiang production and construction corps(2020AB013)

摘要/Abstract

摘要：

【目的】比较两种酵母培养物及其代谢成分的差异,为指导酵母培养物的生产及应用提供参考。【方法】材料为1种自研酵母培养物与达农威益康 XP酵母培养物,利用非靶标代谢组学UHPLC-QTOF-MS技术,分析比较二者代谢产物成分及差异。【结果】(1)在二级类别注释下,正、负离子模式分别注释到614、497个化合物,主要代谢类别为有机酸,核苷、核苷酸及其衍生物,氨基酸及其衍生物,两种酵母培养物均无特有代谢成分,只在含量上有显著(P<0.05)差异。(2)共237个差异代谢物在正离子模式检出,176个显著(P<0.05)上调表达,61个显著(P<0.05)下调表达;136个差异代谢物在负离子模式检出,64个显著(P<0.05)上调表达,72个显著(P<0.05)下调表达。(3)差异代谢物的 KEGG(Kyoto Encyclopedia of Genes and Genomes)富集分析主要集中在丙氨酸、天冬氨酸和谷氨酸等代谢通路。【结论】自研酵母培养物与达农威益康XP的代谢产物含量存在显著差异,但其发酵原料及工艺更简便,并含有多种具药理、生理作用的代谢成分,有潜在应用价值。

关键词: 酵母培养物; 非靶标代谢组学; 差异代谢物; 酿酒酵母

Abstract:

【Objective】This project aims to compare the metabolic components differences between the XP and experimental group yeast culture,the findings will provide a reference for guiding the production and application of yeast culture.【Methods】The untargeted metabolomics UHPLC-QTOF-MS technology was used to analyze and compare the composition and differences of the metabolic components between the 2 yeast cultures.【Results】(1) Under the super classification annotation, a total of 614 and 497 compounds were noted in positive and negative ion modes respectively.The main metabolic classes were organic acids, nucleosides, nucleotides and their derivatives, amino acids and their derivatives and so on.There were no specific metabolic components in the two yeast cultures, only significant differences in content (P<0.05).(2) In the positive ion mode, 237 differential metabolites were detected, of which 176 were significantly up-regulated(P<0.05) and 61 were significantly down-regulated (P<0.05) in expression.In the negative ion mode, 136 differential metabolites were detected, of which 64 were significantly up-regulated (P<0.05) and 72 were significantly down-regulated (P<0.05) in expression.(3) The KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment pathway of differential metabolites mainly focused on alanine, aspartate and glutamate metabolism.【Conclusion】Due to different carbon and nitrogen sources and fermentation conditions, the yeast cultures have significant differences in the content of metabolites, but with their own advantages of the biological activity of main differential metabolites.

Key words: yeast culture; untargeted metabolomics; differential metabolite; Saccharomyces cerevisiae

中图分类号:

S188

林青, 时红玲, 秦新政, 李月, 王子涵, 高雁, 曾军, 王浩中, 娄恺, 霍向东. 基于非靶向代谢组学分析两种酵母培养物的成分差异[J]. 新疆农业科学, 2024, 61(5): 1218-1226.

LIN Qing, SHI Hongling, QIN Xinzheng, LI Yue, WANG Zihan, GAO Yan, ZENG Jun, WANG Haozhong, LOU Kai, HUO Xiangdong. Analysis of the composition differences between two yeast cultures based on untargeted metabolomics[J]. Xinjiang Agricultural Sciences, 2024, 61(5): 1218-1226.

图/表 7

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模式 Modes	名称 Names	Log- FC value	P- value	VIP	上调/ 下调 Up/ Down
POS	染料木素	6.10	0.00	1.23	Up
POS	甘氨酰-L-亮氨酸	6.09	0.00	1.21	Up
POS	大豆苷元	5.79	0.01	1.23	Up
POS	异亮氨酸-脯氨酸	5.40	0.00	1.23	Up
POS	尿素	5.25	0.00	1.23	Up
POS	L-阿拉伯糖	4.98	0.00	1.23	Up
POS	5-甲氧基尿苷	4.75	0.00	1.12	Up
POS	5-甲氧基色胺	4.70	0.00	1.23	Up
POS	二氢辣椒素	4.10	0.00	1.23	Up
POS	1-甲基腺苷	4.09	0.00	1.23	Up
POS	1-棕榈酰甘油	-5.03	0.00	1.23	Down
POS	三甲氧苯酰胺	-4.20	0.01	1.22	Down
NEG	白杨素	5.15	0.01	1.22	Up
NEG	甲苯赖氨酸氯甲基酮	4.86	0.00	1.22	Up
NEG	3-羟基十二酸	4.66	0.00	1.18	Up
NEG	秦皮素	4.20	0.01	1.21	Up
NEG	N6,N6,N6- 三甲基-L-赖氨酸	-9.75	0.00	1.22	Down
NEG	硫酸雄甾酮	-5.94	0.00	1.22	Down
NEG	1-油酰-sn- 甘油3-磷酸	-4.45	0.01	1.22	Down
NEG	4-羟基肉桂酸	-4.39	0.01	1.21	Down
NEG	2-氧代己二酸	-4.27	0.00	1.21	Down
NEG	1-棕榈酰溶血磷脂酸	-4.11	0.01	1.22	Down

模式 Modes	名称 Names	Log- FC value	P- value	VIP	上调/ 下调 Up/ Down
POS	染料木素	6.10	0.00	1.23	Up
POS	甘氨酰-L-亮氨酸	6.09	0.00	1.21	Up
POS	大豆苷元	5.79	0.01	1.23	Up
POS	异亮氨酸-脯氨酸	5.40	0.00	1.23	Up
POS	尿素	5.25	0.00	1.23	Up
POS	L-阿拉伯糖	4.98	0.00	1.23	Up
POS	5-甲氧基尿苷	4.75	0.00	1.12	Up
POS	5-甲氧基色胺	4.70	0.00	1.23	Up
POS	二氢辣椒素	4.10	0.00	1.23	Up
POS	1-甲基腺苷	4.09	0.00	1.23	Up
POS	1-棕榈酰甘油	-5.03	0.00	1.23	Down
POS	三甲氧苯酰胺	-4.20	0.01	1.22	Down
NEG	白杨素	5.15	0.01	1.22	Up
NEG	甲苯赖氨酸氯甲基酮	4.86	0.00	1.22	Up
NEG	3-羟基十二酸	4.66	0.00	1.18	Up
NEG	秦皮素	4.20	0.01	1.21	Up
NEG	N6,N6,N6- 三甲基-L-赖氨酸	-9.75	0.00	1.22	Down
NEG	硫酸雄甾酮	-5.94	0.00	1.22	Down
NEG	1-油酰-sn- 甘油3-磷酸	-4.45	0.01	1.22	Down
NEG	4-羟基肉桂酸	-4.39	0.01	1.21	Down
NEG	2-氧代己二酸	-4.27	0.00	1.21	Down
NEG	1-棕榈酰溶血磷脂酸	-4.11	0.01	1.22	Down

基于非靶向代谢组学分析两种酵母培养物的成分差异

Analysis of the composition differences between two yeast cultures based on untargeted metabolomics

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