大灰优蚜蝇雌成虫足感器扫描电镜分析

doi:10.6048/j.issn.1001-4330.2024.04.018

新疆农业科学 ›› 2024, Vol. 61 ›› Issue (4): 937-944.DOI: 10.6048/j.issn.1001-4330.2024.04.018

• 植物保护·微生物·农业装备工程与机械化 • 上一篇下一篇

大灰优蚜蝇雌成虫足感器扫描电镜分析

吴基楠¹^,²(), 董婉莹²^,³, 刘同先¹, 王冰²(), 王桂荣²()

1.青岛农业大学植物医学学院,山东青岛 266109
2.中国农业科学院植物保护研究所植物病虫害综合治理全国重点实验室,北京 100193
3.浙江省农业科学院植物保护与微生物研究所,杭州 310021

收稿日期:2023-08-11 出版日期:2024-04-20 发布日期:2024-05-31
通信作者: 王桂荣(1972-),男,安徽宿松人,研究员,研究方向为化学生态学,(E-mail)wangguirong@caas.cn；
王冰(1983-),女,河北宣化人,研究员,研究方向为化学生态学,(E-mail)wangbing02@caas.cn
作者简介:吴基楠(1997-),男,福建三明人,硕士研究生,研究方向为化学生态学,(E-mail)wujinan107@163.com
基金资助:
国家自然科学基金面上项目(32272621);国家自然科学基金重点项目(32130089);中国烟草总公司重大科技项目(110202201017(LS-01)

Ultrastructural observations of leg sensilla in Eupeodes corollae using scanning electron microscopy

WU Jinan¹^,²(), DONG Wanying²^,³, LIU Tongxian¹, WANG Bing²(), WANG Guirong²()

1. College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao Shandong 266109, China
2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
3. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2023-08-11 Published:2024-04-20 Online:2024-05-31
Correspondence author: WANG Guirong (1972-), male, from Susong, Anhui,researcher, research direction: chemical ecology, (E-mail)wangguirong@caas.cn；
WANG Bing (1983-), female, from Xuanhua, Hebei, researcher, research direction:chemical ecology, (E-mail)wangbing02@caas.cn
Supported by:
The General Program of National Natural Science Foundation of China(32272621);The Key Program of National Natural Science Foundation of China(32130089);The Major scientific and technological projects of China Tobacco Corporation (110202201017(LS-01)

摘要/Abstract

摘要：

【目的】分析大灰优蚜蝇Eupeodes corollae雌成虫胸足的感器类型、特征及分布,为天敌昆虫食蚜蝇产卵选择机制奠定形态学基础。【方法】利用扫描电镜技术观察其足上感器的超微结构。【结果】大灰优蚜蝇雌成虫胸足由基节、转节、腿节、胫节、跗节和前跗节6个部分组成,其上分布5种类型的感器,分别为锥形感器(3种亚型I~III)、毛形感器(长毛形感器2种亚型I~II;短毛形感器2种亚型I~II)、刺形感器(2种亚型I~II)、Böhm氏鬃毛和微毛感器。锥形感器仅分布于足跗节,其中锥形感器I亚型顶端具孔,在化学感受过程中发挥重要的作用;毛形感器和刺形感器在雌成虫胸足腿节、胫节和跗节均有分布,这2种类型感器的毛干表面有纵脊,无壁孔,基部具可活动的臼状窝,是典型的机械感器;Böhm 氏鬃毛和微毛感器数量较多,散布在各类感器之间。【结论】在大灰优蚜蝇雌成虫胸足上鉴定了5种类型的感器,足跗节上的感器类型和数量最多,是足上重要的化学感受区域。

关键词: 大灰优蚜蝇; 足跗节; 锥形感器; 超微结构; 化学感受; 机械感受

Abstract:

【Objective】 The objective of this study is to elucidate the types, features, and distribution of leg sensilla in female adult Eupeodes corollae, in the hope of providing a morphological foundation for further studying on the egg-laying selection mechanism of the natural enemy E. corollae. 【Methods】 Using scanning electron microscopy, the ultrastructure of leg sensilla in female E. corollae adults was observed. 【Results】 The results showed that the legs of female adults of E. corollae consisted of six segments, including coxa, trochanter, femur, tibia, tarsus, and pretarsus. In total, five types of sensilla were distributed on the legs: sensilla basiconica (subtypes I, II, and III), sensilla trichodea (long sensilla trichodea subtypes I and II; short sensilla trichodea subtypes I and II), sensilla chaetica (subtypes I and II), Böhm bristles, and microtrichiae. Sensilla basiconica (SB) were exclusively located on the tarsus, and one subtype SB I had a pore at its tip, suggesting a crucial function in chemoreception. Sensilla trichodea and sensilla chaetica were distributed on the femur, tibia, and tarsus of legs in female adult. These two types of sensilla had the longitudinal furrows on their hairy surface, no wall pore, and a movable socket at the base, which were characteristic features of mechanosensilla. The number of Böhm bristles and microtrichiae were large and widely distributed around various types of sensilla. 【Conclusion】 In this study, five types of sensilla are identified on the leg of female E. corollae. The abundance and diverse types of sensilla are found in the tarsus segments of the legs, denoting their critical role in chemosensory perception.

Key words: Eupeodes corollae; tarsus; sensilla basiconica; ultrastructure; chemosensation; mechanosensation

中图分类号:

Q964
S43

吴基楠, 董婉莹, 刘同先, 王冰, 王桂荣. 大灰优蚜蝇雌成虫足感器扫描电镜分析[J]. 新疆农业科学, 2024, 61(4): 937-944.

WU Jinan, DONG Wanying, LIU Tongxian, WANG Bing, WANG Guirong. Ultrastructural observations of leg sensilla in Eupeodes corollae using scanning electron microscopy[J]. Xinjiang Agricultural Sciences, 2024, 61(4): 937-944.

图/表 4

图1 大灰优蚜蝇雌虫前足扫描电镜图注:A. 大灰优蚜蝇足部分整体图,由腿节、胫节、跗节和前跗节构成;B. 腿节整体图;C. 胫节整体图;D. 跗节整体图;E. 前跗节整体图(黄色矩形边框);腿节Femur(Fe);胫节Tibia(Ti);跗节Tarsus(Tar);前跗节Pretarsus(Pretar)

Fig.1 Scanning electron micrographs of foreleg in female adults of Eupeodes corollae Note: A. General view of the part of leg from Eupeodes corollae, showing the femur, the tibia, the tarsus, the pretarsus; B. General view of the femur; C. General view of the tibia; D. General view of the tarsus; E. General view of the pretarsus (yellow rectangular border); Femur (Fe); Tibia (Ti); Tarsus (Tar); Pretarsus (Pretar)

图2 大灰优蚜蝇雌虫前足腿节和胫节扫描电镜图注:A. 位于腿节的毛形感器;B. 长毛形感器 I型long sensilla trichodea I(LST I)和短毛形感器 I型short sensilla trichodea I(SST I);C. Böhm 氏鬃毛(BB),毛形感器(ST)基部的表皮窝;D. 位于胫节的刺形感器(SC)和Böhm 氏鬃毛(BB)

Fig.2 Scanning electron micrographs of femur and tibia in foreleg of female adults of E. corollae Note: A. Sensilla trichodea in the femur; B. Long sensilla trichodea I (LST I) and short sensilla trichodea I (SST I); C. Böhm bristles (BB), epidermal fossa at the base of sensilla trichodea (ST); D. Sensilla chaetica (SC) and böhm bristles (BB) at the tibia

图3 大灰优蚜蝇雌虫前足跗节扫描电镜图注:A. 位于跗节背面的短刺形感器short sensilla chaetica(SSC)和长刺形感器long sensilla chaetica(LSC);B. 位于跗节两侧的短刺形感器(SSC)和长刺形感器(LSC);C. 位于跗节的短刺形感器(SSC)和Böhm 氏鬃毛(BB);D. 微毛感器(Mt);E. 位于跗节腹面的长毛形感器 Ⅱ型long sensilla trichodea Ⅱ(LST Ⅱ)和短毛形感器 Ⅱ型short sensilla trichodea Ⅱ(SST Ⅱ);F. 跗节腹面的锥形感器(SB)SB I,SB II和SB III亚型;G. 锥形感器 I型(SB I);H. 锥形感器 II型(SB II);I. 锥形感器 III型(SB III)

Fig.3 Scanning electron micrographs of tarsus in foreleg of female adults of E. corollae Note: A. Short sensilla chaetica (SSC) and long sensilla chaetica (LSC) at the back of the tarsus; B. Short sensilla chaetica (SSC) and long sensilla chaetica (LSC) at the both sides of the tarsus; C. Short sensilla chaetica (SSC) and böhm bristles (BB) at the tarsus; D. Microtrichiae (Mt); E. Long sensilla trichodea Ⅱ (LST Ⅱ) and short sensilla trichodea Ⅱ (SST Ⅱ) at the ventral sides of the tarsus; E. SB I, SB II and SB III at the ventral sides of the tarsus; G. SB I; H. SB II; I. SB III

图4 大灰优蚜蝇雌虫前足前跗节扫描电镜图注:A. 黄色框为前跗节的腹面图;B. 黄色框为前跗节的背面图;C. 位于前跗节背面的爪(claw)和刚毛(seta);D. 位于前跗节腹面的爪垫(puvillus)

Fig.4 Scanning electron micrographs of pretarsus in foreleg of female adults of E. corollae Note: A. General view of ventral view of the pretarsus (yellow rectangular border); B. General view of back view of the pretarsus (yellow rectangular border); C. Claw and seta at the back side of the pretarsus; D. Puvillus at the ventral side of the pretarsus

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大灰优蚜蝇雌成虫足感器扫描电镜分析

Ultrastructural observations of leg sensilla in Eupeodes corollae using scanning electron microscopy

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