新疆农业科学 ›› 2024, Vol. 61 ›› Issue (1): 241-252.DOI: 10.6048/j.issn.1001-4330.2024.01.026
经宏鑫1(), 李佳豪1, 郭庆勇2, 钟明彬1, 张城铭1, 马壮1, 阿布都沙拉木·艾合麦提1, 李晓斌1,2(
)
收稿日期:
2023-04-22
出版日期:
2024-01-20
发布日期:
2024-02-21
通信作者:
李晓斌(1988-),男,甘肃天水人,副教授,硕士生导师,研究方向为草食动物营养代谢,(E-mail)172387243@qq.com作者简介:
经宏鑫(2000-),男,吉林四平人,硕士研究生,研究方向为动物营养与饲料,(E-mail)309221066@qq.com
基金资助:
JING Hongxin1(), LI Jiahao1, GUO Qingyong2, ZHONG Mingbin1, ZAHNG Chengming1, MA Zhuang1, Abudoushalamu Aihemaiti1, LI Xiaobin1,2(
)
Received:
2023-04-22
Published:
2024-01-20
Online:
2024-02-21
Supported by:
摘要:
【目的】放牧条件下为泌乳母马补喂氨基酸,分析补喂对哺乳马驹生长发育及血液生化指标的影响,为哺乳马驹在母马挤乳条件下健康生长提供参考依据。【方法】选择产驹日期相近(5月),泌乳30 d的伊犁马母马24匹,年龄7~9岁,胎次4~5胎,产驹至30 d日均8 h挤奶量为(2.79±0.02) kg。24匹马驹平均体重为(71.36±14.3) kg,8母16公。结合马驹性别将24匹马驹随机分为4个组,每组6匹(2母,4公),分别为对照组、试验Ⅰ组、试验Ⅱ组和试验Ⅲ组,马驹分组与母马相对应。在相同放牧条件下(放牧时间、饮水时间、挤奶时间和放牧草场完全相同),对照组不进行任何氨基酸补喂,试验Ⅰ组补喂赖氨酸40 g/(d·匹)+苏氨酸20 g/(d·匹),试验Ⅱ组补喂赖氨酸60 g/(d·匹)+苏氨酸40 g/(d·匹),试验Ⅲ组补喂赖氨酸80 g/(d·匹)+苏氨酸60 g/(d·匹)。进行为期为120 d的补饲试验。【结果】(1)试验Ⅰ组和试验Ⅱ组与对照组相比能够极显著提高哺乳马驹的60 d体重、60 d体高、90 d体高、总增高、30 d体斜长和120 d体斜长,分别提高了8.37%、4.44%、4.27%、24.22%、5.31%、5.20%和6.25%、4.05%、4.09%、22.14%、5.55%、6.22%(P<0.01)。试验Ⅰ组能够显著提高哺乳马驹60 d胸围、90 d胸围、120 d胸围和胸围总增长,分别提高了6.34%、9.38%、9.52%和33.83%(P<0.05);试验Ⅲ组仅能够显著提高哺乳马驹60 d的体高、61~90 d和91~120 d胸围增长,分别提高3.53%(P<0.01)、25.17%(P<0.05)和21.26%(P<0.05),另外,各试验组在管围相关指标与对照组均无显著差异(P>0.05)。(2)各试验组哺乳马驹血液中 Glu、ALT、AST 和 ALP 的含量与对照组相比分别极显著提高了 13.08%、 33.91%、26.93%、6.19%、17.08%、38.18%、26.69%、9.43%和 12.02%、38.14%、18.38%、6.35%(P<0.01),同时,试验Ⅰ组和试验Ⅱ组哺乳马驹血液中 TP、UA、T-Bil、γ-GT 和 Ca2+的含量分别极显著提高了 6.19%、23.44%、29.89%、15.47%、15.02% 和 6.67%、31.18%、30.07%、20.02%、14.00%(P<0.01)。【结论】在放牧条件下为泌乳母马补喂赖氨酸和苏氨酸,能够显著促进哺乳马驹体重、体尺的增长,同时提高马驹血液中TP、ALB、GLB、Glu和Ca2+的含量,增加ALT、AST和ALP的活力,进而促进马驹的生长发育,补喂泌乳母马40~60 g/(d·匹)赖氨酸和20~40 g/(d·匹)苏氨酸,能够促进马驹的生长发育和机体健康。
中图分类号:
经宏鑫, 李佳豪, 郭庆勇, 钟明彬, 张城铭, 马壮, 阿布都沙拉木·艾合麦提, 李晓斌. 放牧条件下泌乳母马补喂氨基酸对哺乳马驹生长发育及血液生化指标的影响[J]. 新疆农业科学, 2024, 61(1): 241-252.
JING Hongxin, LI Jiahao, GUO Qingyong, ZHONG Mingbin, ZAHNG Chengming, MA Zhuang, Abudoushalamu Aihemaiti, LI Xiaobin. Effects of supplemental feeding amino acids on growth and blood biochemical indices of lactating foals under grazing conditions[J]. Xinjiang Agricultural Sciences, 2024, 61(1): 241-252.
试验天数 Test days(d) | 对照组 Control group | 试验Ⅰ组 Test group Ⅰ | 试验Ⅱ组 Test group Ⅱ | 试验Ⅲ组 Test group Ⅲ |
---|---|---|---|---|
0 | 3.34±0.44 | 3.35±1.22 | 3.36±0.34 | 3.36±0.91 |
0~120 | 3.33±0.11d | 4.03±0.52bB | 4.21±0.45aA | 3.88±0.32cB |
表1 试验期母马日均8 h挤奶量
Tab.1 Average daily 8 h milking volume of mares during the experiment period(kg)
试验天数 Test days(d) | 对照组 Control group | 试验Ⅰ组 Test group Ⅰ | 试验Ⅱ组 Test group Ⅱ | 试验Ⅲ组 Test group Ⅲ |
---|---|---|---|---|
0 | 3.34±0.44 | 3.35±1.22 | 3.36±0.34 | 3.36±0.91 |
0~120 | 3.33±0.11d | 4.03±0.52bB | 4.21±0.45aA | 3.88±0.32cB |
项目 Iems | 含量 Content (%) | 营养水平 Nutrient levels (2) | 含量 Content (%) |
---|---|---|---|
大麦 Barley | 55.54 | 干物质DM | 89.25 |
玉米 Corn | 36.00 | 粗蛋白质CP | 14.56 |
豆粕 Soybean meal | 6.00 | 粗脂肪EE | 4.08 |
磷酸氢钙 Calcium hydrogen carbonate | 1.00 | 粗灰分Ash | 2.82 |
预混料 Pre mixed material(1) | 1.16 | 中性洗涤纤维NDF | 13.36 |
酸性洗涤纤维ADF | 5.18 | ||
钙Ca | 0.31 | ||
磷P | 0.46 | ||
消化能DE(MJ/kg)(2) | 16.62 | ||
合计Total | 100.00 |
表2 精料补充料组成及营养水平(干物质基础)
Tab.2 Composition and nutrient level of concentrate supplement (dry matter basis)
项目 Iems | 含量 Content (%) | 营养水平 Nutrient levels (2) | 含量 Content (%) |
---|---|---|---|
大麦 Barley | 55.54 | 干物质DM | 89.25 |
玉米 Corn | 36.00 | 粗蛋白质CP | 14.56 |
豆粕 Soybean meal | 6.00 | 粗脂肪EE | 4.08 |
磷酸氢钙 Calcium hydrogen carbonate | 1.00 | 粗灰分Ash | 2.82 |
预混料 Pre mixed material(1) | 1.16 | 中性洗涤纤维NDF | 13.36 |
酸性洗涤纤维ADF | 5.18 | ||
钙Ca | 0.31 | ||
磷P | 0.46 | ||
消化能DE(MJ/kg)(2) | 16.62 | ||
合计Total | 100.00 |
试验期 Trial period | 对照组 Control group | 试验Ⅰ组 Test group Ⅰ | 试验Ⅱ组 Test group Ⅱ | 试验Ⅲ组 Test group Ⅲ |
---|---|---|---|---|
0 d(kg) | 70.40±20.16 | 71.80±14.75 | 71.50±15.20 | 72.00±7.55 |
30 d(kg) | 80.75±13.20 | 84.70±18.66 | 85.50±22.34 | 84.40±19.70 |
60 d(kg) | 99.38±13.76Cc | 107.70±16.02Aa | 106.00±21.38ABa | 102.60±23.59BCb |
90 d(kg) | 116.63±12.15b | 128.64±6.42a | 125.40±3.71ab | 122.71±4.80ab |
120 d(kg) | 133.88±10.77b | 144.91±3.58a | 139.41±5.34ab | 138.43±3.33ab |
0~30 d增重 0-30 days to gain weight(kg) | 17.25±2.25b | 22.77±3.22a | 21.00±3.85ab | 18.38±2.39ab |
31~60 d增重 31-60 days to gain weight(kg) | 18.63±0.85Bb | 23.40±1.92Aa | 19.30±2.59Bb | 18.20±2.02Bb |
61~90 d增重 61-90 days to gain weight(kg) | 17.25±2.25b | 20.94±3.18a | 19.40±1.83ab | 20.11±1.67ab |
91~120 d增重 91-120 days to gain weight(kg) | 15.75±2.47 | 17.93±3.53 | 16.21±2.29 | 16.68±1.21 |
总增重Total weight gain(kg) | 70.38±6.42b | 78.97±2.52a | 74.64±4.30ab | 73.02±2.32ab |
平均日增重Average daily gain(kg) | 0.57±0.06Bb | 0.66±0.01Aa | 0.63±0.01ABa | 0.62±0.02ABab |
表3 泌乳母马补喂氨基酸下哺乳马驹体重变化
Tab.3 Effects of amino acid supplementation in lactating mares on body weight of lactating foals
试验期 Trial period | 对照组 Control group | 试验Ⅰ组 Test group Ⅰ | 试验Ⅱ组 Test group Ⅱ | 试验Ⅲ组 Test group Ⅲ |
---|---|---|---|---|
0 d(kg) | 70.40±20.16 | 71.80±14.75 | 71.50±15.20 | 72.00±7.55 |
30 d(kg) | 80.75±13.20 | 84.70±18.66 | 85.50±22.34 | 84.40±19.70 |
60 d(kg) | 99.38±13.76Cc | 107.70±16.02Aa | 106.00±21.38ABa | 102.60±23.59BCb |
90 d(kg) | 116.63±12.15b | 128.64±6.42a | 125.40±3.71ab | 122.71±4.80ab |
120 d(kg) | 133.88±10.77b | 144.91±3.58a | 139.41±5.34ab | 138.43±3.33ab |
0~30 d增重 0-30 days to gain weight(kg) | 17.25±2.25b | 22.77±3.22a | 21.00±3.85ab | 18.38±2.39ab |
31~60 d增重 31-60 days to gain weight(kg) | 18.63±0.85Bb | 23.40±1.92Aa | 19.30±2.59Bb | 18.20±2.02Bb |
61~90 d增重 61-90 days to gain weight(kg) | 17.25±2.25b | 20.94±3.18a | 19.40±1.83ab | 20.11±1.67ab |
91~120 d增重 91-120 days to gain weight(kg) | 15.75±2.47 | 17.93±3.53 | 16.21±2.29 | 16.68±1.21 |
总增重Total weight gain(kg) | 70.38±6.42b | 78.97±2.52a | 74.64±4.30ab | 73.02±2.32ab |
平均日增重Average daily gain(kg) | 0.57±0.06Bb | 0.66±0.01Aa | 0.63±0.01ABa | 0.62±0.02ABab |
试验期 Trial period | 对照组 Control group | 试验Ⅰ组 Test group Ⅰ | 试验Ⅱ组 Test group Ⅱ | 试验Ⅲ组 Test group Ⅲ |
---|---|---|---|---|
0 d(cm) | 101.20±8.87 | 101.63±2.06 | 100.50±3.11 | 100.25±1.71 |
30 d(cm) | 103.63±6.49 | 106.40±3.51 | 105.75±1.26 | 105.00±4.24 |
60 d(cm) | 106.50±3.46Bb | 111.45±1.23Aa | 111.00±1.41Aa | 110.40±1.14Aa |
90 d(cm) | 111.62±2.55Bb | 116.60±1.08Aa | 116.38±2.29Aa | 114.10±1.34ABab |
120 d(cm) | 117.12±0.61b | 122.98±4.73a | 121.28±2.85ab | 119.09±3.28ab |
0~30 d增高0-30 d increase(cm) | 4.88±2.06 | 4.88±4.25 | 5.25±3.77 | 5.67±2.31 |
31~60 d增高31-60 dincrease(cm) | 5.00±1.00 | 5.31±1.11 | 5.25±0.96 | 5.50±0.58 |
61~90 d增高61-90 d dincrease(cm) | 4.28±0.66 | 5.38±0.63 | 5.38±1.11 | 4.17±0.76 |
91~120 d增高91-120 d dincrease(cm) | 4.50±1.41 | 5.10±0.79 | 4.90±0.91 | 4.62±0.42 |
总增高Total increase in height(cm) | 16.18±2.21Bb | 21.35±0.61Aa | 20.78±2.56Aab | 18.84±0.30ABab |
表4 泌乳母马补喂氨基酸下哺乳马驹体高变化
Tab.4 Effects of amino acid supplementation in lactating mares on body height of lactating foals
试验期 Trial period | 对照组 Control group | 试验Ⅰ组 Test group Ⅰ | 试验Ⅱ组 Test group Ⅱ | 试验Ⅲ组 Test group Ⅲ |
---|---|---|---|---|
0 d(cm) | 101.20±8.87 | 101.63±2.06 | 100.50±3.11 | 100.25±1.71 |
30 d(cm) | 103.63±6.49 | 106.40±3.51 | 105.75±1.26 | 105.00±4.24 |
60 d(cm) | 106.50±3.46Bb | 111.45±1.23Aa | 111.00±1.41Aa | 110.40±1.14Aa |
90 d(cm) | 111.62±2.55Bb | 116.60±1.08Aa | 116.38±2.29Aa | 114.10±1.34ABab |
120 d(cm) | 117.12±0.61b | 122.98±4.73a | 121.28±2.85ab | 119.09±3.28ab |
0~30 d增高0-30 d increase(cm) | 4.88±2.06 | 4.88±4.25 | 5.25±3.77 | 5.67±2.31 |
31~60 d增高31-60 dincrease(cm) | 5.00±1.00 | 5.31±1.11 | 5.25±0.96 | 5.50±0.58 |
61~90 d增高61-90 d dincrease(cm) | 4.28±0.66 | 5.38±0.63 | 5.38±1.11 | 4.17±0.76 |
91~120 d增高91-120 d dincrease(cm) | 4.50±1.41 | 5.10±0.79 | 4.90±0.91 | 4.62±0.42 |
总增高Total increase in height(cm) | 16.18±2.21Bb | 21.35±0.61Aa | 20.78±2.56Aab | 18.84±0.30ABab |
试验期 Trial period | 对照组 Control group | 试验Ⅰ组 Test group Ⅰ | 试验Ⅱ组 Test group Ⅱ | 试验Ⅲ组 Test group Ⅲ |
---|---|---|---|---|
0 d (cm) | 83.50±7.72 | 83.75±4.79 | 83.00±7.83 | 83.33±4.73 |
30 d (cm) | 90.67±0.58Bc | 95.75±1.50Aa | 96.00±0.82Aa | 93.20±2.28ABb |
60 d (cm) | 98.90±0.70 | 102.50±1.29 | 101.25±4.86 | 99.25±0.96 |
90 d (cm) | 104.77±1.38 | 107.20±1.64 | 106.80±1.64 | 106.00±1.80 |
120 d (cm) | 108.63±0.32Bb | 114.59±1.71Aa | 115.88±1.31Aa | 108.67±1.61Bb |
0~30 d增长0-30 d growth(cm) | 8.80±0.84b | 10.75±0.96a | 11.00±1.00a | 9.75±1.71ab |
31~60 d增长31-60 d growth(cm) | 7.00±0.85 | 7.25±0.50 | 7.25±1.89 | 7.50±0.71 |
61~90 d增长61-90 d growth(cm) | 5.70±0.14 | 6.80±1.48 | 5.00±1.41 | 5.75±2.33 |
91~120 d增长91-120 d growth(cm) | 3.87±0.31b | 5.84±0.59a | 5.00±1.00ab | 4.00±1.00b |
体斜长总增长Total additive oblique length(cm) | 24.97±4.43Bb | 30.84±1.01ABa | 32.88±3.12Aa | 25.33±1.26Bb |
表5 泌乳母马补喂氨基酸下哺乳马驹体斜长变化
Tab.5 Effects of amino acid supplementation in lactating mares on oblique length of lactating foals
试验期 Trial period | 对照组 Control group | 试验Ⅰ组 Test group Ⅰ | 试验Ⅱ组 Test group Ⅱ | 试验Ⅲ组 Test group Ⅲ |
---|---|---|---|---|
0 d (cm) | 83.50±7.72 | 83.75±4.79 | 83.00±7.83 | 83.33±4.73 |
30 d (cm) | 90.67±0.58Bc | 95.75±1.50Aa | 96.00±0.82Aa | 93.20±2.28ABb |
60 d (cm) | 98.90±0.70 | 102.50±1.29 | 101.25±4.86 | 99.25±0.96 |
90 d (cm) | 104.77±1.38 | 107.20±1.64 | 106.80±1.64 | 106.00±1.80 |
120 d (cm) | 108.63±0.32Bb | 114.59±1.71Aa | 115.88±1.31Aa | 108.67±1.61Bb |
0~30 d增长0-30 d growth(cm) | 8.80±0.84b | 10.75±0.96a | 11.00±1.00a | 9.75±1.71ab |
31~60 d增长31-60 d growth(cm) | 7.00±0.85 | 7.25±0.50 | 7.25±1.89 | 7.50±0.71 |
61~90 d增长61-90 d growth(cm) | 5.70±0.14 | 6.80±1.48 | 5.00±1.41 | 5.75±2.33 |
91~120 d增长91-120 d growth(cm) | 3.87±0.31b | 5.84±0.59a | 5.00±1.00ab | 4.00±1.00b |
体斜长总增长Total additive oblique length(cm) | 24.97±4.43Bb | 30.84±1.01ABa | 32.88±3.12Aa | 25.33±1.26Bb |
试验期 Trial period | 对照组 Control group | 试验Ⅰ组 Test group Ⅰ | 试验Ⅱ组 Test group Ⅱ | 试验Ⅲ组 Test group Ⅲ |
---|---|---|---|---|
0 d (cm) | 95.75±10.59 | 95.25±5.25 | 94.25±8.77 | 96.00±4.24 |
30 d (cm) | 98.00±8.28 | 101.00±8.63 | 101.60±9.29 | 100.60±9.46 |
60 d (cm) | 104.52±8.61b | 111.60±1.67a | 108.00±2.89ab | 107.00±2.35ab |
90 d (cm) | 108.92±9.01b | 120.20±5.64ab | 112.20±6.72ab | 112.70±6.25ab |
120 d(cm) | 113.32±9.52b | 125.25±7.24a | 117.40±4.74ab | 118.80±7.16ab |
0~30 d增长0-30 d growth(cm) | 5.50±0.58 | 7.50±4.04 | 7.50±2.08 | 7.33±5.77 |
31~60 d增长31-60 d growth(cm) | 7.50±1.54 | 9.25±4.19 | 7.25±3.57 | 8.10±1.95 |
61~90 d增长61-90 d growth(cm) | 4.40±1.13Bb | 6.50±0.50Aa | 4.63±1.25Bb | 5.88±0.47ABa |
91~120 d增长91-120 d growth(cm) | 4.40±1.13Bc | 7.60±1.14Aa | 5.75±0.65ABbc | 6.10±1.08ABb |
胸围总增长Total increase in chest size(cm) | 19.85±8.16b | 30.00±3.81a | 24.88±5.86ab | 23.38±4.27ab |
表6 泌乳母马补喂氨基酸下哺乳马驹胸围变化
Tab.6 Effects of amino acid supplementation in lactating mares on breast circumference of lactating foals
试验期 Trial period | 对照组 Control group | 试验Ⅰ组 Test group Ⅰ | 试验Ⅱ组 Test group Ⅱ | 试验Ⅲ组 Test group Ⅲ |
---|---|---|---|---|
0 d (cm) | 95.75±10.59 | 95.25±5.25 | 94.25±8.77 | 96.00±4.24 |
30 d (cm) | 98.00±8.28 | 101.00±8.63 | 101.60±9.29 | 100.60±9.46 |
60 d (cm) | 104.52±8.61b | 111.60±1.67a | 108.00±2.89ab | 107.00±2.35ab |
90 d (cm) | 108.92±9.01b | 120.20±5.64ab | 112.20±6.72ab | 112.70±6.25ab |
120 d(cm) | 113.32±9.52b | 125.25±7.24a | 117.40±4.74ab | 118.80±7.16ab |
0~30 d增长0-30 d growth(cm) | 5.50±0.58 | 7.50±4.04 | 7.50±2.08 | 7.33±5.77 |
31~60 d增长31-60 d growth(cm) | 7.50±1.54 | 9.25±4.19 | 7.25±3.57 | 8.10±1.95 |
61~90 d增长61-90 d growth(cm) | 4.40±1.13Bb | 6.50±0.50Aa | 4.63±1.25Bb | 5.88±0.47ABa |
91~120 d增长91-120 d growth(cm) | 4.40±1.13Bc | 7.60±1.14Aa | 5.75±0.65ABbc | 6.10±1.08ABb |
胸围总增长Total increase in chest size(cm) | 19.85±8.16b | 30.00±3.81a | 24.88±5.86ab | 23.38±4.27ab |
试验期 Trial period | 对照组 Control group | 试验Ⅰ组 Test group Ⅰ | 试验Ⅱ组 Test group Ⅱ | 试验Ⅲ组 Test group Ⅲ |
---|---|---|---|---|
0 d (cm) | 12.80±1.30 | 12.81±0.24 | 12.75±0.96 | 12.50±0.58 |
30 d (cm) | 13.17±0.91 | 13.75±0.29 | 13.58±0.43 | 13.20±0.47 |
60 d (cm) | 14.47±0.92 | 15.10±0.73 | 14.55±0.31 | 14.50±0.41 |
90 d (cm) | 15.73±0.57 | 16.05±0.95 | 15.80±0.53 | 15.78±0.26 |
120 d (cm) | 16.90±0.52 | 17.18±1.10 | 17.00±0.83 | 16.98±0.21 |
0~30 d增长0-30 d growth(cm) | 1.10±0.14 | 1.00±0.00 | 1.10±0.36 | 1.10±0.61 |
31~60 d增长31-60 d growth(cm) | 1.08±0.40 | 1.28±0.75 | 1.18±0.76 | 1.14±0.44 |
61~90 d增长61-90 d growth(cm) | 1.08±0.13 | 1.13±0.23 | 1.18±0.46 | 1.20±0.26 |
91~120 d增长91-120 d growth(cm) | 1.05±0.10 | 1.13±0.39 | 1.06±0.52 | 1.06±0.36 |
总增管围Total additional pipe circumference | 3.90±0.61 | 4.36±1.31 | 4.25±1.10 | 4.05±1.42 |
表7 泌乳母马补喂氨基酸下哺乳马驹管围变化
Tab.7 Effects of amino acid supplementation in lactating mares on tube girth of lactating foals
试验期 Trial period | 对照组 Control group | 试验Ⅰ组 Test group Ⅰ | 试验Ⅱ组 Test group Ⅱ | 试验Ⅲ组 Test group Ⅲ |
---|---|---|---|---|
0 d (cm) | 12.80±1.30 | 12.81±0.24 | 12.75±0.96 | 12.50±0.58 |
30 d (cm) | 13.17±0.91 | 13.75±0.29 | 13.58±0.43 | 13.20±0.47 |
60 d (cm) | 14.47±0.92 | 15.10±0.73 | 14.55±0.31 | 14.50±0.41 |
90 d (cm) | 15.73±0.57 | 16.05±0.95 | 15.80±0.53 | 15.78±0.26 |
120 d (cm) | 16.90±0.52 | 17.18±1.10 | 17.00±0.83 | 16.98±0.21 |
0~30 d增长0-30 d growth(cm) | 1.10±0.14 | 1.00±0.00 | 1.10±0.36 | 1.10±0.61 |
31~60 d增长31-60 d growth(cm) | 1.08±0.40 | 1.28±0.75 | 1.18±0.76 | 1.14±0.44 |
61~90 d增长61-90 d growth(cm) | 1.08±0.13 | 1.13±0.23 | 1.18±0.46 | 1.20±0.26 |
91~120 d增长91-120 d growth(cm) | 1.05±0.10 | 1.13±0.39 | 1.06±0.52 | 1.06±0.36 |
总增管围Total additional pipe circumference | 3.90±0.61 | 4.36±1.31 | 4.25±1.10 | 4.05±1.42 |
项目 Items | 对照组 Control group | 试验Ⅰ组 Test group Ⅰ | 试验Ⅱ组 Test group Ⅱ | 试验Ⅲ组 Test group Ⅲ | P值P-value | ||
---|---|---|---|---|---|---|---|
T | D | T×D | |||||
总蛋白TP(g/L) | 54.57±6.71Bb | 58.17±6.70Aa | 58.47±5.67Aa | 55.89±6.80ABab | 0.006 | 0.000 | 0.003 |
白蛋白ALB(g/L) | 26.83±5.21 | 28.34±3.55 | 28.36±5.16 | 27.81±5.12 | 0.456 | 0.000 | 0.195 |
球蛋白GLB(g/L) | 27.74±7.77 | 29.83±4.92 | 30.11±4.95 | 28.08±6.73 | 0.227 | 0.025 | 0.000 |
尿酸UA(μmol/L) | 15.58±7.62Bb | 20.35±6.13Aa | 22.64±8.86Aa | 21.27±6.94Aa | 0.001 | 0.000 | 0.040 |
葡萄糖Glu(mmol/L) | 5.05±1.03Bb | 5.81±0.88Aa | 6.09±0.89Aa | 5.74±0.96Aa | 0.000 | 0.000 | 0.231 |
甘油三酯TG(mmol/L) | 0.26±0.11ab | 0.24±0.07b | 0.23±0.08b | 0.30±0.15a | 0.029 | 0.000 | 0.047 |
总胆红素T-Bil(μmol/L) | 11.35±4.49Bb | 16.19±6.94Aa | 16.23±5.84Aa | 13.10±4.48ABb | 0.000 | 0.024 | 0.028 |
谷丙转氨酶ALT(U/L) | 10.72±3.84Bb | 16.22±10.24Aa | 17.34±5.21Aa | 17.33±6.76Aa | 0.000 | 0.000 | 0.054 |
谷草转氨酶AST(U/L) | 255.76±37.93Cc | 350.00±57.27Aa | 348.87±83.85Aa | 313.36±67.69Bb | 0.000 | 0.000 | 0.004 |
碱性磷酸酶ALP(U/L) | 570.53±28.70Bc | 608.16±44.07Ab | 629.91±62.23Aa | 609.19±38.38Ab | 0.000 | 0.000 | 0.033 |
γ-谷氨酰转移酶 γ-GT(U/L) | 19.18±4.07Cc | 22.69±3.78ABab | 23.98±4.03Aa | 20.85±5.26BCbc | 0.000 | 0.001 | 0.044 |
肌酸激酶CK(U/L) | 123.42±21.90Bbc | 115.08±26.16Bc | 164.84±23.59Aa | 128.74±22.79Bab | 0.000 | 0.489 | 0.680 |
乳酸脱氢酶LDH(U/L) | 416.93±43.14 | 406.54±51.22 | 407.34±37.84 | 423.49±47.82 | 0.368 | 0.297 | 0.042 |
钙Ca2+(mmol/L) | 2.15±0.54Bb | 2.53±0.34Aa | 2.50±0.42Aa | 2.42±0.42ABa | 0.001 | 0.000 | 0.431 |
无机磷P(mmol/L) | 2.27±0.55 | 2.53±0.50 | 2.45±0.49 | 2.54±0.63 | 0.126 | 0.007 | 0.012 |
镁Mg2+(mmol/L) | 1.43±0.63Aa | 1.20±0.35Bb | 1.02±0.25Bb | 1.12±0.40Bb | 0.000 | 0.000 | 0.000 |
铁Fe2+(μmol/L) | 44.11±10.38Aa | 49.06±11.46Aa | 49.14±10.66Aa | 36.90±10.52Bb | 0.0000 | 0.011 | 0.290 |
表8 泌乳母马补喂氨基酸下哺乳马驹血液生化指标变化
Tab.8 Effects of amino acid supplementation in lactating mares on blood biochemical indexes of lactating foals
项目 Items | 对照组 Control group | 试验Ⅰ组 Test group Ⅰ | 试验Ⅱ组 Test group Ⅱ | 试验Ⅲ组 Test group Ⅲ | P值P-value | ||
---|---|---|---|---|---|---|---|
T | D | T×D | |||||
总蛋白TP(g/L) | 54.57±6.71Bb | 58.17±6.70Aa | 58.47±5.67Aa | 55.89±6.80ABab | 0.006 | 0.000 | 0.003 |
白蛋白ALB(g/L) | 26.83±5.21 | 28.34±3.55 | 28.36±5.16 | 27.81±5.12 | 0.456 | 0.000 | 0.195 |
球蛋白GLB(g/L) | 27.74±7.77 | 29.83±4.92 | 30.11±4.95 | 28.08±6.73 | 0.227 | 0.025 | 0.000 |
尿酸UA(μmol/L) | 15.58±7.62Bb | 20.35±6.13Aa | 22.64±8.86Aa | 21.27±6.94Aa | 0.001 | 0.000 | 0.040 |
葡萄糖Glu(mmol/L) | 5.05±1.03Bb | 5.81±0.88Aa | 6.09±0.89Aa | 5.74±0.96Aa | 0.000 | 0.000 | 0.231 |
甘油三酯TG(mmol/L) | 0.26±0.11ab | 0.24±0.07b | 0.23±0.08b | 0.30±0.15a | 0.029 | 0.000 | 0.047 |
总胆红素T-Bil(μmol/L) | 11.35±4.49Bb | 16.19±6.94Aa | 16.23±5.84Aa | 13.10±4.48ABb | 0.000 | 0.024 | 0.028 |
谷丙转氨酶ALT(U/L) | 10.72±3.84Bb | 16.22±10.24Aa | 17.34±5.21Aa | 17.33±6.76Aa | 0.000 | 0.000 | 0.054 |
谷草转氨酶AST(U/L) | 255.76±37.93Cc | 350.00±57.27Aa | 348.87±83.85Aa | 313.36±67.69Bb | 0.000 | 0.000 | 0.004 |
碱性磷酸酶ALP(U/L) | 570.53±28.70Bc | 608.16±44.07Ab | 629.91±62.23Aa | 609.19±38.38Ab | 0.000 | 0.000 | 0.033 |
γ-谷氨酰转移酶 γ-GT(U/L) | 19.18±4.07Cc | 22.69±3.78ABab | 23.98±4.03Aa | 20.85±5.26BCbc | 0.000 | 0.001 | 0.044 |
肌酸激酶CK(U/L) | 123.42±21.90Bbc | 115.08±26.16Bc | 164.84±23.59Aa | 128.74±22.79Bab | 0.000 | 0.489 | 0.680 |
乳酸脱氢酶LDH(U/L) | 416.93±43.14 | 406.54±51.22 | 407.34±37.84 | 423.49±47.82 | 0.368 | 0.297 | 0.042 |
钙Ca2+(mmol/L) | 2.15±0.54Bb | 2.53±0.34Aa | 2.50±0.42Aa | 2.42±0.42ABa | 0.001 | 0.000 | 0.431 |
无机磷P(mmol/L) | 2.27±0.55 | 2.53±0.50 | 2.45±0.49 | 2.54±0.63 | 0.126 | 0.007 | 0.012 |
镁Mg2+(mmol/L) | 1.43±0.63Aa | 1.20±0.35Bb | 1.02±0.25Bb | 1.12±0.40Bb | 0.000 | 0.000 | 0.000 |
铁Fe2+(μmol/L) | 44.11±10.38Aa | 49.06±11.46Aa | 49.14±10.66Aa | 36.90±10.52Bb | 0.0000 | 0.011 | 0.290 |
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摘要 173
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