一株无柄灵芝菌(G.resinaceum)LZ02高产漆酶液体培养基的响应面法优化

doi:10.6048/j.issn.1001-4330.2020.05.010

新疆农业科学 ›› 2020, Vol. 57 ›› Issue (5): 869-876.DOI: 10.6048/j.issn.1001-4330.2020.05.010

一株无柄灵芝菌(G.resinaceum)LZ02高产漆酶液体培养基的响应面法优化

谢玉清, 代金平, 陈竞, 王志方, 古丽努尔·艾合买提, 杨新平, 张慧涛, 王小武, 冯蕾

新疆农业科学院微生物应用研究所/新疆特殊环境微生物重点实验室,乌鲁木齐 830091

收稿日期:2019-11-06 发布日期:2020-04-23
作者简介:谢玉清(1976-),女,新疆乌鲁木齐人,硕士,研究方向为蓄禽养殖废弃物综合利用,(E-mail)604778481@qq.com
基金资助:
国家自然科学基金(2166038);新疆维吾尔自治区重点研发项目(2017B01003);新疆维吾尔自治区重点研发项目(2017B01001)

Optimizing Carbon and Nitrogen Source in Liquid Fermentation Medium of G.resinaceum LZ02 Producing Laccase with Response Surface Methodology

Xie Yuqing, DAI Jinping, Chen Jin, Wang Zhifang, GuliAhmat, Yang Xinping, Zhang Huitao, Wang Xiaowu, Feng Lei

Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, /Xinjiang Key Laboratory of Special Environmental Microbiology, Urumqi 830091, China

Received:2019-11-06 Online:2020-04-23
Supported by:
Supported by:The National Natural Science Foundation of China(2166038);Key Research and Development Projects of Xin Jiang(2017B01003)

摘要/Abstract

摘要： 【目的】采用响应面法优化液体发酵培养基的碳、氮源,提高大型真菌无柄灵芝菌(G.resinaceum)LZ02的漆酶产量。【方法】 在MF基础产酶培养基和单因素试验的基础上,以干酪素(A)、麦麸(B)、葡萄糖(C)、酒石酸铵(D)的添加量为4个影响因素,以漆酶酶活为响应值(Y),采用Design-Expert8软件的中心组合设计方法设计响应面试验,研究干酪素、麦麸、葡萄糖、酒石酸铵各自变量及其交互作用对漆酶产量的影响,并建立以漆酶产量为响应值的二次多项式回归数学模型,得到高产漆酶的最佳液体发酵培养基的碳氮源配比。【结果】无柄灵芝菌(G.resinaceum)LZ02产酶培养基4个因素的最佳组合为干酪素含量2.5%、麦麸含量1.5%、葡萄糖含量1.0%和酒石酸铵含量0.6%时,相应响应面二次模型预测漆酶活性最大值为16505U/L,通过3次平行摇瓶发酵试验得到的菌漆酶活性为15 800 U/L,与理论预测值无明显差异。【结论】响应面分析法提供的模型较真实地拟合了实际情况,证明了模型的可靠性。

关键词: 无柄灵芝菌LZ02; 漆酶; 响应面法; 液体培养基优化

Abstract: 【Objective】 To improve the laccase yield of G.resinaceum LZ02, fermentation liquid medium producing Laccase was optimized by response surface methodology on the basis of MF medium.【Method】Conducting a central composite design using response surface methodology with factors including casein, wheat bran, glucose and ammonium tartrate and taking enzyme activity as response values .【Result】 The enzyme activity of G.resinaceum LZ02 was predicted to be up 16505U/L when optimal group of four factors is 2.5% casein,1.5% wheat bran ,1.0% glucose and 0.6% ammonium tartrate ,measured value is 15800 U/L by three parallel test and there is no difference between both values too.【Conclusion】 The data acquisition by response surface methodology matches actually with practical data and the model was proved to be reliable.

Key words: G.resinaceum LZ02; laccase; response surface methodology; optimizing fermentation liquid medium

中图分类号:

S188

谢玉清, 代金平, 陈竞, 王志方, 古丽努尔·艾合买提, 杨新平, 张慧涛, 王小武, 冯蕾. 一株无柄灵芝菌(G.resinaceum)LZ02高产漆酶液体培养基的响应面法优化[J]. 新疆农业科学, 2020, 57(5): 869-876.

Xie Yuqing, DAI Jinping, Chen Jin, Wang Zhifang, GuliAhmat, Yang Xinping, Zhang Huitao, Wang Xiaowu, Feng Lei. Optimizing Carbon and Nitrogen Source in Liquid Fermentation Medium of G.resinaceum LZ02 Producing Laccase with Response Surface Methodology[J]. Xinjiang Agricultural Sciences, 2020, 57(5): 869-876.

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一株无柄灵芝菌(G.resinaceum)LZ02高产漆酶液体培养基的响应面法优化

Optimizing Carbon and Nitrogen Source in Liquid Fermentation Medium of G.resinaceum LZ02 Producing Laccase with Response Surface Methodology

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