体外产气法评价黄芩秸秆对绵羊体外瘤胃发酵特性的影响

Evaluation of the effect of Scutellaria baicalensis straw on rumen fermentation characteristics of sheep in vitro

    摘要
  • 摘要:
    目的 为解决黄芩秸秆堆放、焚烧等利用不当，大量废弃的环境生态问题，利用体外产气法评价黄芩秸秆对绵羊体外瘤胃发酵特性的影响，为黄芩秸秆在绵羊实际生产中的科学应用提供参考。
    方法 试验采用体外产气法探讨不同水平黄芩秸秆对体外发酵的影响，发酵底物为含 0%、4%、8%、12%、16% 和 20% 黄芩秸秆的等能等氮饲粮，测定 6、12、24、36、48 和 72 h 的产气量，以及 72 h 后的营养物质降解率和体外瘤胃发酵参数。
    结果  12% 和 20% 试验组的 GP6 h 较其他组显著升高（ P<0.05），且各试验组的 GP6 h 均呈线性变化（ P<0.05）；GP72 h 呈现 12% 试验组 >8% 试验组 > 对照组 >16% 试验组 >4% 试验组 >20% 试验组，但差异不显著（ P>0.05）。 干物质降解率黄芩秸秆添加组均高于对照组，其中 12% 试验组干物质降解率显著高于对照组（ P<0.05）；粗蛋白质降解率 4% 和 12% 试验组显著高于其他试验组（ P<0.05），16% 和 20% 试验组显著低于其他试验组（ P<0.05）；且随着黄芩秸秆比例的增加，各试验组干物质降解率和粗蛋白质降解率均呈二次函数变化（ P<0.05）。各试验组间发酵液 pH 值在 6.63~6.77 之间。各试验组氨态氮浓度差异不显著（ P>0.05）。总挥发性脂肪酸 12% 试验组 >8% 试验组 >4% 试验组 > 对照组 >16% 试验组 >20% 试验组，但差异不显著（ P>0.05）。
    结论 在本试验条件下，较对照组而言， 8%~12% 黄芩秸秆饲粮的体外产气量和底物降解速率较好，黄芩秸秆添加水平为 12% 时效果最优。

     

    Abstract:
    Objective In order to solve the environmental and ecological problems of improper utilization and waste of Scutellaria baicalensis straw and to develop its potential as a functional feed. The evaluation of the effects of Scutellaria baicalensis straw on in vitro rumen fermentation characteristics in sheep might provide a reference for the scientific application of the straw in the actual production of sheep.
    Methods The effects of different levels of Scutellaria baicalensis straw was studied by in vitro gas production method. The fermentation substrate was iso-nitrogen diet containing 0%, 4%, 8%, 12%, 16% and 20% Scutellaria baicalensis straw. The gas production at 6, 12, 24, 36, 48 and 72 h, as well as the nutrient degradation rate and rumen fermentation parameters after 72 h were measured.
    Results The GP6 h of 12% and 20% groups was significantly higher than those of other groups (P<0.05), and the GP6 h of each group showed a linear change. GP72 h showed 12% > 8% > control group > 16% > 4% > 20%, but the difference was not significant (P>0.05). The dry matter degradation rate of the Scutellaria baicalensis straw group was higher than that of the control group, and the dry matter degradation rate of 12% group was significantly higher than that of the control group (P<0.05). The crude protein degradation rates of 4% and 12% groups were significantly higher than those of other experimental groups (P<0.05), while 16% and 20% groups were significantly lower than other groups (P<0.05). With the increase of the proportion of Scutellaria baicalensis straw, the dry matter degradation rate and crude protein degradation rate of each group changed as a quadratic function (P<0.05). The pH values of fermentation broth among the experimental groups were between 6.63 and 6.77. There was no significant difference in the concentration of ammonia nitrogen among the groups (P>0.05). Total volatile fatty acids were: 12% > 8% > 4% > control group > 16% > 20%, but the difference was not significant (P>0.05).
    Conclusion Under the conditions of this experiment, compared with the control group, the in vitro gas production and substrate degradation rate of 8%~12% Scutellaria baicalensis straw feed is better, and the effect is the best when the adding level of Scutellaria baicalensis straw is 12%.

     

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