Abstract: 【 Objective 】To examine whether the germination characteristics of forage seeds change after undergoing spaceflight-induced mutation in the of providing foundational data for research on seed germination and seedling growth characteristics.【 Methods 】Four types of forage seeds carried on the Shenzhou XIV spacecraft, Alhagi camelorum, Seriphidium transiliense, Krascheninnikovia eversmanniana, and Sorghum sudanense var. Xincao No.1, were subjected to germination tests using the filter paper method. The germination rate, germination potential, seedling root length, seedling shoot length, and vitality index were measured and statistically analyzed.【 Results 】Compared to the unmutated control seeds（CK）, the germination rate and index of Krascheninnikovia eversmanniana significantly decreased by 70.00% and 67.36%, respectively, while the rootto-shoot ratio significantly increased by 10.00%（P<0.001）. After spaceflight-induced mutation, he germination index of Artemisia yili decreased significantly by 27.36%，and the root-to-shoot ratio of Seriphidium transiliense significantly increased by 242.00%（P<0.001）, with no significant changes in other indicators. Alhagi camelorum showed improvements in germination rate, germination potential, germination index, root-to-shoot ratio, and fresh weight, though the differences were not significant. Post-mutation, Sorghum sudanense var. Xincao No.1 showed an increase in all measures except for a 31.00% decrease in the root-to-shoot ratio, with no significant changes otherwise.【 Conclusion 】The germination characteristics of the seeds have changed after spaceflight-induced mutation, significantly affecting germination.