Abstract: 【Objective】 To explore the effects of microbial microbial agents on the physiological and photosynthetic characteristics of walnut leaves,and to provide scientific basis for the application of microbial microbial agents on walnut in Aksu area. 【Methods】 A total of 6 experimental treatments were set up with 14-year old walnut "Wen185" as the test material,which were 30 g/ strain of Bacillus subtilis (T₁), 30 g/ strain of Trichoderma hartzii (T₂), 40 g/ strain of Bacillus subtilis (T₃), 40 g/ strain of Trichoderma hartzii (T₄), and 30 g/ strain of Trichoderma Hartzii. Bacillus subtilis 20 g/ strain + Trichoderma harziensis 20 g/ strain (T₅), water control (CK). Then, leaf area, leaf length, leaf width, fresh weight and dry weight were measured. After thatn, chlorophyll (SPAD), photosynthetic characteristics and other indicators were determined and the effects of different amounts of microbial inoculants on walnut leaves were analyzed. 【Results】 Under T5 treatment, the area, length, fresh weight and dry weight of walnut leaves were significantly higher than those of control, which were 31.75%,11.55%,90.83% and 166.66% higher, respectively. Leaf nutrient contents of nitrogen, phosphorus and potassium showed the maximum value, which was 65.68%,61.14% and 57.47% higher than that of the control. Chlorophyll content and photosynthetic characteristics index parameters were also increased under this treatment. 【Conclusion】 Trench application of 20 g on each of Bacillus subtilis and Trichoderma harziana promote the growth,nutrient content and photosynthetic performance of walnut leaves. In this experiment, the combination of Bacillus subtilis 20 g and Trichoderma harziana 20 g has the best effect.