Abstract: 【Objective】 This project aims to compare the metabolic components differences between the XP and experimental group yeast culture,the findings will provide a reference for guiding the production and application of yeast culture. 【Methods】 The untargeted metabolomics UHPLC-QTOF-MS technology was used to analyze and compare the composition and differences of the metabolic components between the 2 yeast cultures. 【Results】 (1) Under the super classification annotation, a total of 614 and 497 compounds were noted in positive and negative ion modes respectively.The main metabolic classes were organic acids, nucleosides, nucleotides and their derivatives, amino acids and their derivatives and so on.There were no specific metabolic components in the two yeast cultures, only significant differences in content (P<0.05).(2) In the positive ion mode, 237 differential metabolites were detected, of which 176 were significantly up-regulated(P<0.05) and 61 were significantly down-regulated (P<0.05) in expression.In the negative ion mode, 136 differential metabolites were detected, of which 64 were significantly up-regulated (P<0.05) and 72 were significantly down-regulated (P<0.05) in expression.(3) The KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment pathway of differential metabolites mainly focused on alanine, aspartate and glutamate metabolism. 【Conclusion】 Due to different carbon and nitrogen sources and fermentation conditions, the yeast cultures have significant differences in the content of metabolites, but with their own advantages of the biological activity of main differential metabolites.