Abstract:
【Objective】 To optimize the extraction conditions of polysaccharides from the wild boletus in Aksu region and determine the physical properties and antioxidant capacity of different polysaccharide components.
【Methods】 The wild boletus was sampled from Aksu region,and water extraction conditions of polysaccharide were optimized. Two polysaccharide components,LD-40 and LD-80,were obtain by fractional precipitation of ethanol with volume fraction of 40% and 80%. The polysaccharide properties of water solubility,water retention and viscosity were tested. The antioxidant capacity was evaluated by free radical scavenging of DPPH and hydroxyl and reducing power experiments.
【Results】 The optimal extraction conditions were solid-liquid ratio 1∶30,temperature at 80℃,time for 3 h,then the yield of polysaccharide was 13.36%. Polysaccharide components,LD-40 and LD-80,obtained from ethanol fractional precipitation accounted for 92.37% and 7.63% of the total polysaccharide,respectively. The water solubility of LD-40 and LD-80 was 66.9% and 74.9%,respectively,and the water holding capacities were 7.71 and 6.16 g/g,respectively. At the same concentration,the viscosity of LD-40 was stronger than that of LD-80,but LD-80 had stronger antioxidant capacity than LD-40.
【Conclusion】 The wild boletus in Aksu region is rich in polysaccharides which has good water holding capacity,but the antioxidant activity is weak. The results can provide scientific reference for the further development and utilization of wild boletus polysaccharides.