Abstract: 【Objective】 To study the cold resistance and adaptability to low temperature of trichome germplasm.So as to provide a scientific reference for the development and utilization of resources in the next step. 【Methods】 In this experiment, the characteristic variety of Qinghai province downy linear pepper cultivar ‘Qingxianjiao 2’ was used as the test material. The plants were treated with low temperature at seedling stage, and the activity of superoxide dismutase (SOD), the content of soluble sugar, soluble protein content and malondialdehyde (MDA) were determined under different treatments. 【Results】 The results showed that with the extension of stress time, the activity of superoxide dismutase (SOD), the trend of soluble sugar, soluble protein content were firstly increased and then decreased, while the trend of malondialdehyde (MDA) content was firstly decreased and then increased and again decreased. With the decrease of treatment temperature, the changes of different physiological indexes in each treatment group tended to be gentle. Correlation analysis showed that soluble sugar content was significantly negatively correlated with soluble protein content, and malondialdehyde content was significantly positively correlated with soluble protein content. 【Conclusion】 Combined with the significant difference and correlation analysis of each index, the purpose is to evaluate the cold resistance of special germplasm resources through biochemical indicators, indicating that hairy germplasm resources have better cold resistance and adaptability to low temperature.