Abstract: 【Objective】 To select the dominant varieties suitable for popularization in Xinjiang by analyzing the differences of nutritional composition, composition characteristics and comprehensive evaluation results based on quality of different varieties of mulberry so as to provide reference for variety optimization, new variety cultivation and multiple utilization of fruit mulberry resources. 【Methods】 6 farm varieties and 2 introduced varieties in Xinjiang were taken as experimental materials to determine 33 quality indexes, respectively.Correlation analysis, cluster analysis, principal component analysis and orthogonal partial least square-discriminant analysis were used to distinguish the differences of nutritional quality and key characteristic indexes of mulberry.Finally, the varieties with the best quality were selected according to the score of comprehensive evaluation. 【Results】 The basic nutritional components of different mulberries had certain differences and strong correlation, and there were variations in different degrees; all mulberries contained higher rutin and chlorogenic acid, among which the total phenolic acid contents of medicinal mulberry and Taiwan black were higher, 17 single amino acids were detected in 8 kinds of mulberries, and the changes of Asp and Glu were the most drastic and the average content was high.The highest content of TAAs was black mulberry, NEAAs was the main component of mulberry amino acid, followed by MAAs, and the lowest was CEAAs.Different mulberry varieties were divided into 4 groups by cluster analysis, and 11 different quality indexes were obtained by OPLS-DA analysis.The first five principal components were extracted by principal component analysis, and a comprehensive evaluation mathematical model was constructed: D_ensemble =0.474,8F₁+0.219,7F₂+0.142,6F₃+0.097,2F₄+0.065,8F₅, and the final comprehensive evaluation score of mulberry could be obtained by calculation. 【Conclusion】 According to the comprehensive evaluation, the top three are black mulberry, black pearl and Taiwan black mulberry, which can be used as the dominant fruit mulberry varieties suitable for popularization in Xinjiang.