Abstract: 【Objective】 The research laid a foundation for the function research of high quality gene of long-staple cotton and broad genetic basis of upland cotton. 【Methods】 In this study, Xinjiang’s main early-middle-maturing upland cotton variety Xinluzhong 60 was used as the female parent and the high-quality island cotton variety Xinhai 41 was used as the male parent. Then, the BC₁F₂ population composed of 151 individual plants combined with Xinluzhong 60 as the recurrent parent was used as the mapping population to construct a genetic linkage map. We excavated stable QTLs related to fiber quality to provide a basis for molecular marker-assisted selection breeding. The linkage map was constructed with SSR markers by Join Map 4.0 software. The QTLs for fiber quality properties in BC₁F₂ populations were mapped by the inclusive composite interval mapping method with Ici Mapping. 【Results】 The map included 52 SSR loci and 14 linkage groups, covering 824 cM and accounting for 18.5% of the cotton genome. And the longest linkage group is 150.3 cM, containing 6 markers, and the shortest is 0.3 cM, containing 2 markers.A QTL related to the average length of the upper half of the fiber was detected, located on chromosome 14. It can be used as a candidate gene for functional research of cotton fiber quality genes in land-sea introgression lines. 【Conclusion】 The polymorphic markers selected in this study can assist early breeding selection.