Abstract: 【Objective】 To explore the effects of soil sealing treatments with six herbicides on the germination rate, plant height, cotyledon length, cotyledon width, average radicle length and other biological indicators of Isatis indigotica during the seedling stage, and select the best one from the six in the hope of providing a theoretical basis for its field production. 【Methods】 In the seedling stage of I indigotica, the effects of 6 herbicides on the germination rate and average radicle length of Isatis indica at 24-192 h at different application concentrations were studied by indoor pot culture and petri dish culture. 【Results】 Germination rate: the concentration of refined metolachlor, oxyfluorfen, sec-butadiene, and flumioxazin were not significantly different, and did not affect the emergence of Isatis indigotica. The concentration of the agent higher than 80 mL/667m² Pendimethalin had an inhibitory effect on the germination rate of Isatis indigotica. The higher the concentration of Promethazine, the more obvious the inhibition of the germination; the average radicle length: metolachlor, sec-butaline, oxyfluorfen, Promethazine, and flumioxazin have no effect on the growth of radicles. As the concentration of pendimethalin increased, the inhibition of radicle growth was becoming stronger; plant height: high concentration of refined metolachlor at 30 mL/667m², had an inhibitory effect on the plant height. Pendimethalin 80-200 mL/667m² treatment had a significant inhibitory effect on the plant height. F-butaline had no effect on the plant height. Ether has an inhibitory effect on the plant height at the concentration of 40-70 mL/667m². Promatoxin had an inhibitory effect on the plant height at the concentration of 80-200 mL/667m². When the concentration of flumioxazin was 3-7 g/667m², the inhibitory effect on the plant height of was not obvious; Cotyledon length: the concentration of metolachlor, pendimethalin, f-butadiene, oxyfluorfen, and flumioxazin had no effect on the cotyledon length. As the application concentration of Promatogin increased, it inhibited the cotyledon length; Cotyledon width: the 5 kinds of amine herbicides: metolachlor, pendimethalin, sec-butadiene, oxyfluorfen had no effect on the cotyledon width. Promatogin at 120-200 mL/667m² concentration would inhibit the cotyledon width. 【Conclusion】 Through the experiment of indoor pot and petri dish culture, it can be concluded that only the treatments of f-butaline and flumioxazin have no significant effect on the plant height of Isatis indigotica seedlings; the treatments of pendimethalin and promethazine will affect the growth of isatis germination rate: Pendimethalin treatment will affect the root length growth of Isatis indigotica seedlings; Promethazine treatment will affect the growth of cotyledon length and width of Isatis indigotica. Therefore, under indoor conditions, fine metolachlor, sec-butadiene and flumioxazin have less stress on the growth of Isatis indigotica seedlings.