Abstract: 【Objective】 To understand the resistance to common antimicrobial agents and the carrying of related drug resistance genes of E.coli derived from swine in a large scale pig farm in Aksu region of Xinjiang and to explore the relationship between drug-resistant phenotypes and drug-resistant genes. 【Method】 In this study, the agar dilution method was used to determine the minimum inhibitory concentration of 13 kinds of antimicrobial agents commonly used in pig farms for 443 strains of Escherichia coli isolated and identified from pigs. And the PCR method was used to detect 10 resistance genes in 4 classes of 13 antimicrobial agents. 【Result】 The resistance results showed that the resistance rate of E.coli from swine to 8 kinds of antibiotics was over 70.0%, and the resistance rate to enrofloxacin, streptomycin, spectinomycin, aureomycin and oxytetracycline was over 90.0%, and it had good sensitivity to amikacin, ampomycin, cefotaxime, polymyxin E, the drug resistance rate was not more than 25.0%. The multidrug resistance results was concentrated in 7-9, accounting for 63.6%. The results of drug resistance gene detection showed that: with the exception of tetK gene, all the other 9 drug resistance genes were detected, mainly carrying floR (66.4%) and ant (3 ")-Ia (66.4%)resistance genes. 【Conclusion】 E.coli derived from swine in a large-scale pig farm in Aksu region, Xinjiang, showed resistance to at least one antibacterial drug tested, the multi-drug resistance is serious, the drug resistance spectrum is wide, and the drug resistance gene carrying rate is high. It is suggested that the transmission mechanism of drug-resistant E. coli should be monitored at the gene level, and in addition, the relevant departments should strengthen the standardized use of antibacterial drugs.