Abstract: 【Objective】 To explore the effects of different nitrogen application rates on the production performance, silage raw material quality and nitrogen use efficiency of silage barley in northern Xinjiang, a two-factor randomized block design was used, four nitrogen application rates (0 (N0), 40 (N40), 80 (N80) and 160 kg/hm² (N160).) were set in this experiment. 【Method】 In this study, two barley varieties (Kenmaomai 13 and Ganbei 4) were studied by two-factor random block design, and the nitrogen application rates were 0(N0), 40 (N40), 80(N80) and 160 kg/hm² (N160) to analyze the agronomic characters, dry matter yield, nutritional composition of silage raw materials and nitrogen utilization efficiency of silage barley. 【Result】 The results showed that the agronomic characters, yield, quality and nitrogen use efficiency of silage barley were significantly affected by nitrogen application rate. With the increase of nitrogen application rate, the plant height and total stem number of the two varieties of silage barley increased significantly, and the stem diameter increased at first and then decreased. The threshold value under different nitrogen application rates was: total stem number > plant height > stem diameter. Increasing nitrogen application decreased the proportion of stems and leaves of Kenpimai13 and increased the proportion of tassels, but had no significant effect on Ganpi 4. With the increase of nitrogen application, the hay yield of Kenpimai13 and Ganpi4 increased significantly, which was the highest under N160 treatment, and compared with N0 treatment, the income was increased by 76.1% and 148.7%. Increasing nitrogen application decreased the contents of neutral detergent fiber and acid detergent fiber, increased the content of crude protein, and the content of soluble carbohydrate in Kenpimai13. The partial factor productivity of applying N decreased significantly with the increase of nitrogen application rate. The agricultural nitrogen utilization efficiency of two varieties was the highest under N80 treatment. 【Conclusion】 In order to increase nitrogen use efficiency and obtain high yield and good quality barley, 80 kg/hm² N is recommended to be applied to silage barley in northern Xinjiang.