一种侵染红枣叶片和果实的病原真菌鉴定

Identification of Pathogenic Fungi That Infect Leaves and Fruits of Jujube

  • 摘要: 【目的】研究新疆地区近年发生的一种与枣花叶病有密切关系的病原真菌。【方法】通过病样采集、病原菌分离、回接证病,以及形态观察和rDNA-ITS序列比对等方法,鉴定病原菌。【结果】从有明显花叶症状和褐色叶斑的病叶以及有畸果症状和褐色斑点的病果中分离获得多个真菌单孢菌株,用针刺法将其接种到枣叶和幼果后出现与田间症状基本相似的褐色坏死斑,确定其对枣叶、果的致病性;该菌病原菌形态特征与头状茎点霉基本一致,3个分离菌株的rDNA-ITS序列与头状茎点霉的同源性达到99%。【结论】根据病原菌的形态特征和分子鉴定结果,将该病原菌鉴定为头状茎点霉(Peyronellaea. glomerata)。

     

    Abstract: 【Objective】 In order to clarify the recent occurrence of a species of pathogenic fungi that is closely related to Jujube mosaic disease in Xinjiang.【Method】The pathogen was identified by collecting disease samples, isolating pathogenic bacteria, recovering syndromes, morphological observation and rDNA-ITS sequence alignment.【Result】The spore strains were isolated by some significant mosaic and brown leaf spot samples and abnormal fruit samples. After inoculation of jujube leaves and young fruits with acupuncture, brown necrotic spots similar to the field symptoms were found, and the pathogenicity of them to the leaves and fruits of jujube was determined. The morphological characteristics of the pathogen were basically the same as that of Peyronellaea glomerata, and the homology between the rDNA-ITS sequence of three isolates and that of Peyronellaea glomerata was 99%.【Conclusion】On the basis of morphological and ITS sequence information, the pathogen of the new disease of jujube was identified as Peyronellaea glomerata in Xinjiang.

     

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