Abstract: Objective According to grassland productivity and ecosystem service functions, this project aims to carry out the ecological function regionalization of grassland in Manas River Basin in the hope of providing basis for optimization and utilization of regional grassland resources in the area.MethodAccording to eco-economic property, significance of ecological service value and characteristics of seasonal grazing utilization, regulation mechanism of classified management and utilization was established based on dominant function and spatial and temporal pattern by using interpreted data of remote sensing image and monitoring data of vegetation investigation.Result(1) On the basis of grassland productivity indexes, temperate desert(sandy desert), temperate steppe desert, temperate meadow steppe belonged to the lowest level and mountain meadow and alpine meadow and temperate desert belonged to the highest level.(2) The most important function of ecosystem services was mainly alpine meadow and mountain meadow.(3) Ecological service function could be divided into three types: ecological protection zone (44.0;) that included temperate meadow steppe, mountain meadow and temperate desert (sandy desert), the largest area: the economic functional region that accounted for 48.1;, including temperate desert steppe, alpine meadow, temperate desert and temperate steppe desert, and temperate steppe, which could be utilized as mixed functional region.This region covered a small area, accounting for 7.9;.ConclusionDifferent types of grassland productivity and ecological service function value are different, therefore, the function of ecosystem services and economic development should be taken into comprehensive consideration so that reasonable utilization of the grassland can be achieved.