Abstract:
Objective This project aims to identify the strains that can degrade the low rank coal from the collected samples of coal,rotten wood and soil samples in Xinjiang and study the transformation ability of bacteria to the low rank coal.MethodThe strains were isolated and purified with beef extract peptone culture medium;Through the preliminary screening,the CZAPECK DOX-medium selected became a dark brown or black strain.Then the liquid medium was used to determine the dissolution rate.The phylogenetic tree of 16S rDNA gene was thus constructed for biologically identifying the strains.Result 191 strains of bacteria were isolated from the samples collected,that was 25 strains,61 strains and 105 strains were isolated from the samples of coal,rotten wood and soil,which accounted for 13;,32;,54; of the total,respectively.Through the preliminary screening,28 strains with the function of solubilization of coal were selected,among which,the dissolution rates of NH-6,NF-1,NH-3,YF-3 and NG-4 were 35.06;,34.72;,25.23;,28.03; and 35.89;,respectively.After preliminary identification,NG-4,NF-1 and YF-3 all belonged to Bacillus genus (Bacillus) and NH-3,NH-6 belonged to fake single spore strains (Pseudomonas).ContusionThe selected strains of Bacillus sp.(Bacillus) and Pseudomonas sp.(Pseudomonas) have a higher degradation ability to Xinjiang low rank coal,which supplement the low rank coal degradation bacteria resources in Xinjiang.