Abstract: Objective and MetodIn this experiment,the callus regeneration system of Lavender was established by the use of it as meterial,which layed the basis for fast reproduction of Lavender and genetic transformation.ResultIt was shown that the induction results of the MS +2,4-D 0.10 mg/L + KT0. 30 mg/L medium groups were better than that of other groups.The yellowish callus from 2-3 generations were continuously cultivated on the medium of MS+6-BA 2.0 mg/L+NAA 0.50 mg/L,which form great number of thickly-growing root,when young bud reached 1-2 cm,then was put in medium with MS+6-BA 1.0 mg/L to be expanded for 1-2 generations.After that,the young bud was put in medium with 1/2 MS+IAA 1.0 mg/L to continue to be cultiveated to be rooting.ConclusionIn 20 days,the rooting rate reached 33;.