Abstract: Objective and MethodIn this study 6 genotypes of upland cotton were selected for comparison of their regenerability,including 2 cultivars from Yellow River valley, 3 cultivars from Xinjiang,using a highly responsible genotypes Coker 312 as a material. ResultMost of the genotypes showed high regenerability under the IBA/KT hormone regime.ConclusionUnder the optimized culture procedure, most of the genotypes collected from the two planting areas showed the similar regeneable abilities.The total number of embryos and frequency of embryo germination of "XLZ15"was higher for cotton transformation. This study will improve the transgenic efficiency in Xinjiang.