Abstract: Objective The objective of this study is to identify the pathogens that cause bacterial spot disease of Pleurotus ferulae in Xinjiang.MethodThe causal agents were identified by colony characterization,pathogenicity test,16S rDNA sequence analyses,sequence homology comparison,and biochemical characterization tests.ResultNineteen bacterial isolates were isolated from diseased samples of Pleurotus ferulae.Through pathogenicity test,eight tested isolates caused typical symptoms of bacterial spot disease on the healthy fruiting bodies of Pleurotus ferulae.Based on pathogenicity test,and combined with the results of 16S rDNA sequence analyses,sequence homology comparison,biochemical and molecular characterization,eight isolates were identified as Pseudomonas spp.ConclusionThe bacterial spot disease on Pleurotus ferulae in Xinjiang is incited by Pseudomonas spp.