Abstract: Objective A high performance liquid chromatography (HPLC) method was developed to determine the concentration of four endogenous hormones indole-3-acetic acid (IAA), abscisic acid (ABA), gibberellic acid (GA3), zeatin (ZT) in young fruit of Korla fragrant pear.MethodAfter screening and optimization of conditions, 80; methanol was chosen as extraction solvent.Polyvinylpolypyrrolidone (PVPP) was used to absorb impurities.The samples were extracted with petroleum ether and ethyl acetate.The extracts were purified with a reverse pHase Sep-Pak C18 column (250 mm × 4.6 mm, 5 μm particle size).The column temperature was 40 ℃.The flow rate was 1 mL/min.The injection volume was 10 μL.The mobile phase was 45; methanol (A) and 55; acetic acid (B, pH=3.5).The detection wavelength was 254 nm.ResultZT, GA3, IAA, abscisic acid analysis method of high performance liquid chromatography was established.The HPLC recovery of known concentrations of IAA, ABA, GA3, and ZT ranged between 80.4; and 91.2;.The relative standard deviations (RSDs) ranged between 1.54; and 8.1;.The experimental results were stable and reliable.ConclusionThe HPLC method developed in this study is simple, rapid, reproducible, and accurate.Therefore, it is suitable for rapid and accurate quantification of ZT, GA3, IAA and ABA in pear fruit.