不同提取工艺的新疆一枝蒿乙醇提取液对 H9N2 亚型禽流感病毒的体外作用研究

Study on the effect of Xinjiang Artemisia ethanol extract on H9N2 subtype Avian influenza virus in Vitro

    摘要
  • 摘要:
    目的 研究新疆一枝蒿乙醇提取液体对H9N2亚型禽流感病毒的体外作用效果。
    方法 首先利用加热回流的方式，结合不同的提取条件，提取得到7种新疆一枝蒿乙醇提取液（YZH-C1、YZH-C2、YZH-C3、YZH-C4、YZH-C5、YZH-C6、YZH-C7），后以A549细胞为宿主细胞，从对H9N2亚型禽流感病毒的预防、直接杀灭和治疗作用3个方面探讨不同提取工艺新疆一枝蒿乙醇提取液对H9N2亚型禽流感病毒的作用。
    结果 7种新疆一枝蒿乙醇提取液在A549细胞上的最大无毒浓度分别为31.25、5.00、15.00、8.00、5.00、15.00和0.038 mg/mL；新疆一枝蒿乙醇提取液的最佳作用方式为Ⅱ（药物直接作用）和Ⅲ（治疗性作用）的联合作用方式，对H9N2亚型禽流感病毒具有直接阻断作用及治疗作用。
    结论 新疆一枝蒿乙醇提取液具有抗H9N2亚型禽流感病毒的活性，有望开发成为抗流感病毒药物，后续应进一步分析其抗病毒作用机制。

     

    Abstract:
    Objective Yi-zhi-hao in Xinjiang (Artemisia rupestris L.) belongs to the genus Artemisia of Compositae, which has pharmacological activities such as enhancing immune regulation, anti-inflammation, antibacterial, anti-virus, anti-tumor and so on. In order to study the effect of Xinjiang Artemisia ethanol extract against H9N2 subtype avian influenza virus in vitro.
    Methods Firstly, seven kinds of Xinjiang Artemisia ethanol extract (YZH-C1, YZH-C2, YZH-C3, YZH-C4, YZH-C5, YZH-C6, YZH-C7) were extracted by heating reflux, and then A549 cells were used as host cells. The effect of ethanol extract of Artemisia annua L. from different extraction processes on H9N2 subtype avian influenza virus was discussed from three aspects: prevention, direct killing and therapeutic effect on H9N2 subtype avian influenza virus.
    Results The maximum non-toxic concentrations of seven kinds of Artemisia ethanol extract on A549 cells were 31.25, 5.00, 15.00, 8.00, 5.00, 15.00 and 0.038 mg/mL, respectively. The best action mode of Xinjiang Artemisia ethanol extract was the combination of Ⅱ (drug direct action) and Ⅲ (therapeutic action), which had direct blocking effect and therapeutic effect on H9N2 subtype avian influenza virus.
    Conclusion Xinjiang Artemisia ethanol extract has anti-H9N2 subtype avian influenza virus activity, is expected to develop into anti-influenza virus drugs, and its antiviral mechanism should be further analyzed.

     

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