板蓝根根腐病病原菌的分离鉴定及生物学特性研究

Rot pathogenic bacteria of Isatis indigotica Fort.: isolation and identification, biological characteristics

    摘要
  • 摘要:
    目的 研究板蓝根根腐病病原菌的种类。
    方法 采集新疆 阿勒泰地区哈巴河县和青河县板蓝根根腐病样本，使用组织分离法和稀释涂布分离法进行病原菌分离，并通过致病性测定、形态学、ITA序列分子生物学及生理生化特性对病原菌进行鉴定。
    结果 分离获得6株板蓝根根腐病病原菌，均为真菌，其中菌株B4、B7和B14为腐皮镰孢菌（Fusarium solani），为优势病原菌且致病力较强；菌株B10为尖孢镰刀菌（Fusarium oxysporum），菌株B16为六出花链格孢（Alternaria alstroemeriae），菌株B17为球毛壳菌（Chaetomium globosum），生物学特性研究表明所有供试碳源、氮源均可作为单一碳源、氮源被这6株菌利用，在温度25~37 ℃和pH3~10都能生长。
    结论 板蓝根根腐病的主要致病菌为Fusarium solani、Fusarium oxysporum，并且Alternaria alstroemeriae和Chaetomium globosum在我国是首次报道为板蓝根根腐病原菌，为新疆板蓝根根腐病的快速诊断和防治提供了研究依据。

     

    Abstract:
    Objective To isolate and identify the pathogenic bacteria of the root rot of Isatis indigotica Fort. in Xinjiang.
    Methods The root of Isatis indigotica Fort. were collected from Habahe County and QinHe County, ltacy region, Xinjiang, using tissue isolation and dilution coating isolation methods, and the pathogenic bacteria were identified by pathogenicity determination, morphology, molecular biology of ITS sequences and physiological and biochemical properties.
    Results Six fungi strains were separated from the rot root of Isatis indigotica Fort., the strains B4, B7 and B14 were Fusarium solani, Fusarium solani, which were the dominant pathogen with strong pathogenicity; strain B10 was Fusarium oxysporum, strain B16 was Alternaria alstroemeriae, and strain B17 was Chaetomium globosum. Biological characterization showed that all the carbon and nitrogen sources for the test could be utilized as a single source of carbon and nitrogen by these six strains of bacteria, and they could be grown at temperatures ranging from 25~37 ℃ and pH 3~10.
    Conclusion The main pathogens of the root rot of Isatis indigotica Fort. were Fusarium solani, Fusarium oxysporum in Xinjiang, and Alternaria alstroemeriae and Chaetomium globosum were reported as root rot pathogens for the first time in our country. It provided the research basis for rapid diagnosis and prevention of the root rot of Isatis indigotica Fort. in Xinjiang.

     

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