基于全基因组测序分析贝莱斯芽孢杆菌RT2对辣椒早疫病的作用机制

The analyses of the mechanism of B. velezensis RT2 on early blight of capsicum based on whole genome sequencing

    摘要
  • 摘要:
    目的 分析贝莱斯芽孢杆菌（B. velezensis）RT2基因组序列，探究其对辣椒早疫病菌作用机制。
    方法 采用PacBio测序技术，贝莱斯芽孢杆菌RT2经过组合全基因组，并预测、注释次级代谢产物基因簇。
    结果 RT2全基因组长度、GC含量、编码基因分别为3 932 504 bp、46.53%、3 725个，基因组中tRNA 86个、sRNA 81个、rRNA 27个，在菌株RT2中发掘前噬菌体7个、CRISPR-Cas 2个、基因组岛9个。在数据库NR、GO、KEGG、eggNOG、Pfam和Swiss-Prot中获得基因的功能注释分别为3 711、2 592、2 151、2 961、3 332和2 812个，其中在CA-Zyme中有几丁质酶等酶基因可降解真菌细胞壁。此外，预测了RT2编码抑菌物质基因簇，其中8个在RT2中表现出高度的保守性。
    结论 贝莱斯芽孢杆菌RT2的基因组编码了多个合成基因簇，这些基因簇能够产生抗菌活性次级代谢产物，并且还包含有分解病原真菌细胞壁的关键基因。这些特征是RT2拮抗辣椒早疫病菌的重要因素。

     

    Abstract:
    Objective To analyze the RT2 genome sequence of B. velezensis with a view to exploring the mechanism of its function in early blight of capsicum.
    Methods PacBio sequencing technology was applied, and the whole genome of B. velezensis RT2 was combined to predict and annotate the secondary metabolite gene clusters.
    Results The whole genome length, GC content and coding genes of RT2 were 3 932 504 bp, 46.53%, 3 725, 86 tRNA, 81 sRNA and 27 rRNA, respectively. 7 prophages, 2 CRISPR-Cas and 9 genomic islands were discovered in strain RT2. Functional annotations of 3 711, 2 592, 2 151, 2 961, 3 332 and 2 812 genes were obtained in the NR, GO, KEGG, eggNOG, Pfam and Swiss-Prot databases, respectively. In CA-Zyme, chitinase and other enzyme genes can degrade the fungal cell wall. In addition, gene clusters encoding bacteriostatic substances for RT2 were predicted, eight of which showed high conserved activity in RT2.
    Conclusion The genome of B. velezensis RT2 encodes multiple synthetic gene clusters that produce antimicrobial active secondary metabolites and also contains key genes that break down the cell wall of pathogenic fungi. These characteristics are important factors for RT2 to resist early phytophthora capsicum.

     

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