新疆棉田周边常见杂草上蓟马种类鉴定及其亲缘关系分析

Species identification and phylogenetic analysis of thrips infesting common weeds around cotton fields in Xinjiang

  • 摘要: 【目的】 蓟马是棉花等作物上一种重要的刺吸式害虫,杂草是其重要的中间寄主,在新疆开展棉田周边常见杂草上蓟马种类的鉴定,为棉田内蓟马的综合防控以及科学合理利用棉田周边杂草进行天敌保育提供参考。 【方法】 在新疆棉田外蓟马迁入棉田前的6~7月,从北疆博乐市和呼图壁县以及南疆库尔勒市的棉田周边的19种常见杂草上采集到25份蓟马样本,基于外部形态特征和线粒体细胞色素C氧化酶亚基I(Cytochrome oxidase subunit I,COI)基因的DNA条形码技术进行鉴定和分析,通过Kimura-2-parameter模型计算出种间遗传距离并利用邻接法(Neighbour-Joining tree)构建系统发育树。 【结果】 新疆地区棉田周边大部分常见杂草上采集到的蓟马均为花蓟马Frankliniella intonsa (Trybom),其中紫花苜蓿(Medicago sativa L.)和罗布麻(Apocynum venetum L.)上除了花蓟马外,还杂有少量牛角花齿蓟马Odontothrips loti (Haliday),盐爪爪Kalidium foliatum (Pall.) Moq.上的蓟马属于裸蓟马属Psilothrips sp.。同时,构建的邻接系统发育树表明采自不同地点和不同常见杂草上的花蓟马聚为一支,并与棉花上的花蓟马也聚为一支且种间遗传距离最小。 【结论】 新疆棉田周边多种常见杂草和棉花上的花蓟马具有亲缘关系,且其分布不受地理隔离和杂草寄主的影响。

     

    Abstract: 【Objective】 Thrips is significant piercing-sucking pests of cotton and other crops, with weeds serving as important intermediate hosts. Identifying thrips species infesting common weeds around cotton fields in Xinjiang provides valuable insights for the comprehensive prevention and control of the plant in cotton cultivation. Additionally, this knowledge facilitates the scientific and rational management of weeds around cotton fields to promote the conservation of natural enemies. 【Methods】 25 thrips samples were collected from 19 common weed species around cotton fields in Bole and Hutubi counties in northern Xinjiang, as well as Korla city in southern Xinjiang, during June and July, prior to the migration of thrips into the cotton fields. The samples were identified based on external morphological characteristics and DNA barcoding of the Cytochrome oxidase subunit I (COI) gene. Interspecific genetic distances were calculated using the Kimura-2-parameter model, and a phylogenetic tree was constructed using the Neighbor-Joining method. 【Results】 The results showed that Frankliniellaintonsa (Trybom) was thepredominant thrips species collected from the most common weed hosts in Xinjiang. Additionally, Odontothrips loti (Haliday) was also found on Medicago sativa L., and Apocynumvenetum L. Psilothrips sp., belonging to the genus Psilothrips, were found on Kalidiumfoliatum (Pall.) Moq. Furthermore, neighbor-joining phylogenetic analysis demonstrated that F. intonsa collected from diverse common weeds across different locations formed a distinct cluster, closely related to those obtained from rape and cotton plants, with minimal interspecific genetic distance. 【Conclusion】 Multiple common weeds around cotton fields in Xinjiang serve as hosts for F. intonsa, which infests both these weeds and cotton. The distribution of F. intonsa on a variety of common weeds and cotton in Xinjiang is not influenced by geographical isolation or host specificity.

     

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