Abstract: 【Objective】 To identify and evaluate the salt tolerance of 180 upland cotton germplasm resources at germination stage, screen excellent germplasm, and provide resource materials for the creation of new salt-tolerant germplasm. 【Methods】 In this study, 150 mmol/L NaClsolution was used to simulate salt stress, and 180 germplasm resources were identified and screened for salt tolerance during seed germination. And then, the salt tolerance of these resources was evaluated by principal component analysis and membership function method. 【Results】 Four comprehensive indexes were obtained by principal component analysis, and the comprehensive evaluation D value of salt tolerance was calculated according to the weight. The 180 germplasm resources were divided into five categories by cluster analysis. 【Conclusion】 There are significant differences in relative germination potential and relative germination rate among 180 germplasm resources under salt stress and ten salt-tolerant materials and 31 salt-sensitive materials are obtained by screening.