Abstract: 【Objective】 To screen out the low-temperature tolerant varieties suitable for planting and promotion in the rice producing areas of northern Xinjiang. 【Methods】 The effects of low temperature on antioxidant enzyme activity, yield and constituent factors of rice leaves were studied by using Xinjing 8, Xinjingyi 1, Xinjingyi 7, Akita Komachi and Xindao 11 as experimental materials. 【Results】 The results showed that the POD activity of sword leaf was significantly increased in Xinjing 8 and Akita Komachi after 7 days of low temperature stress at booting stage, the POD activity of sword leaf in Xinjingyi 7 was significantly decreased, and the CAT activity of sword leaf in the three rice varieties after chilling stress was significantly reduced. The low temperature at booting stage extended the number of days of growth period of different rice varieties, significantly reduced the plant height, panicle length and single panicle weight of different rice varieties, and significantly reduced the number of grains per panicle and seed setting rate, which in turn led to the decline of theoretical rice yield. There were differences in the response of 1000-grain weight of different cultivars to low temperature stress, among which there was no difference between Xinjing 8 and Xinjingyi 1 under low temperature stress, while Qiutianxiaoding and Xindao 11 decreased under low temperature stress, and Xinjing 7 increased under low temperature stress. After low temperature stress, the theoretical yield of Qiutianxiaoding was the highest, which was 4,532.75 kg/hm², 56.59%, 98.76%, 3.02% and 1.84% higher than those of Xinjing 8, Xinjingyi 1, Xinjingyi 7 and Xindao 11, respectively. After low temperature stress, the theoretical yield of Xindao 11 was the highest, which was 3988.30 kg/hm², 147.41%, 94.87%, 62.68% and 13.73% higher than those of Xinjing 8, Xinjingyi 1, Xinjingyi 7 and Qiutianxiaoding, respectively. The cold tolerance coefficient of Qiutianxiaoding and xindao 11 was better than those of other varieties. 【Conclusion】 Qiutianxiaoding and Xindao 11 can be used as breeding resources with strong cold tolerance.