基于主成分-隶属函数分析法评价复合菌对小麦根际土壤微生物功能多样性的影响

Evaluation of the effect of composite bacteria on the functional diversity of microorganisms in wheat rhizosphere soils based on principal component-membership function analysis

  • 摘要: 【目的】 基于主成分-隶属函数分析法评价复合菌对小麦根际土壤微生物功能多样性的影响。 【方法】 采用盆栽试验,评价新疆特殊环境中筛选的功能菌株盐单胞菌L6和类芽孢杆菌Y24及复合菌L6-Y24对新疆干旱区小麦根际土壤理化性质和微生物功能多样性的影响。 【结果】 与CK处理相比,功能菌株和复合菌对土壤电导率和pH值未产生显著影响,但土壤速效钾含量分别显著增加了29.17%、47.02%和33.93%(P<0.05),速效氮含量L6-Y24处理显著增加了21.43%(P<0.05)。L6、Y24和L6-Y24处理平均吸光值显著增加了60.04%、74.82%和165.32%(P<0.05),Shannon丰富度指数L6-Y24处理显著增加了15.83%(P<0.05)。复合菌浸种处理对碳水化合物类、氨基酸类、羧酸类、酚酸类、多聚物类和胺类碳源的利用分别显著增加了103.60%、203.72%、208.33%、684.09%、364.10%和100.29%(P<0.05)。菌株及其复合菌对土壤微生物群落功能多样性起分异作用的主要碳源类型为碳水化合物类和羧酸类。促生菌浸种小麦根际土壤理化性质以及微生物功能多样性之间存在密切的关系。 【结论】 复合菌对小麦根际土壤理化性质、微生物功能多样性的提升效果最佳,可以缓解盐碱、干旱等非生物胁迫带来的伤害。

     

    Abstract: 【Objective】 Evaluation of the effect of composite bacteria on the functional diversity of microorganisms in wheat rhizosphere soils based on principal component-membership function analysis. 【Methods】 This study used a pot experiment to investigate the effects of Halomonas L6, Paenibacillus Y24 and compound bacteria L6-Y24 on rhizosphere soil physicochemical properties and microbial functional diversity of wheat in arid areas of Xinjiang. 【Results】 Compared with the control treatment, soaking seeds in L6, Y24, and L6-Y24 hadn't significant impacts on soil electricity conductivity and pH value, but soil available potassium content significantly increased by 29.17%, 47.02%, 33.93%(P<0.05), and available nitrogen content of L6-Y24 significantly increased by 21.43%(P<0.05). Studies of Biolog microbial functional diversity showed that the average absorbance values of L6, Y24 and L6-Y24 had significantly increased by 60.04%, 74.82% and 165.32%(P<0.05), respectively. The Shannon richness index of L6-Y24 had significantly increased by 15.83%. The utilization of carbohydrates, amino acids, carboxylic acids, phenolic acids, polymers and amine carbon sources by composite bacterial soaking significantly increased by 103.60%, 203.72%, 208.33%, 684.09%, 364.10% and 100.29%(P<0.05), respectively. Differentiated functional diversities of the soil microbial communities by functional strains and their complex bacteria were carbohydrates and carboxylic acids. Close relationship among the rhizosphere soil physicochemical properties and microbial function diversities in wheat soaked with growth promoting bacteria. 【Conclusion】 The composite bacteria has the best promoting effects on physicochemical properties and microbial function diversities of wheat rhizosphere soil, and can alleviate the damage caused by abiotic stresses such as salt alkali and drought.

     

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