Abstract: 【Objective】 The objective of this study is twofold: firstly, to examine the impact of elevated temperature stress treatments on the agronomic and physiological characteristics of wheat; and secondly, to identify high-quality, heat-tolerant germplasm resources. 【Methods】 In this study, we analyzed the agronomic traits of 354 spring wheat materials under normal and high temperature stress conditions in 2022 and 2023, performed principal component analysis of heat tolerance coefficients for multiple traits, and identified the heat tolerance of germplasm resources to provide a scientific basis for the selection and breeding of heat-tolerant wheat varieties. 【Results】 High-temperature stress significantly affected (P<0.05) nine characters, including plot yield, thousand grain weight, tolerance, plant height, spike length, total spikelets, SPAD, CT and NDVI, among which heat tolerance coefficients of plot yield were significantly positively correlated with tolerance and negatively correlated with plant height; total spikelets were significantly positively correlated with both plant height and spike length; and NDVI was significantly positively correlated with CT. The composite heat tolerance index H value of each line was calculated by the subordinate function method and analyzed by clustering, and 16 heat tolerant (HT), 127 moderately heat tolerant (MHT), 170 moderately heat sensitive (MHS) and 41 heat sensitive (HS) materials were identified. 【Conclusion】 High temperature stress seriously affects their growth, development and yield. Finally, 10 heat-tolerant materials, such as GCP-9643 and Ningchun 20, and 10 heat-sensitive materials, such as GCP-9670 and sw153, are screened out, which can be used as excellent genetic resources for heat-tolerant wheat breeding and provide the basic materials for creating new wheat varieties with excellent heat-tolerant characteristics.