Abstract: 【Objective】 To effectively control cotton spider mite, this study will be conducted to screen suitable acaricides for the prevention and control of cotton spider mite in the cotton production of the area. 【Methods】 Field efficacy trials were carried out on the main mite species in the Aksu area of southern Xinjiang, namely the Tetranychus truncatus and the Tetranychus dunhuangensis Wang, to determine the acaricidal efficacy of nine chemical agents against cotton spider mite in the area. 【Results】 The research findings revealed that T.dunchuangensis responded exceptionally well to treatments with 30% Tolfenpyrad Suspension Concentrate, 24% Bifenazate spiralate Suspension Concentrate and 40% Phenthoate Fenobucarb Emulsifiable concentrates emulsion after three days. Among them, a 3,000-fold dilution of the 30% etoxazole Suspension control efficacy reached 97.21%. Additionally, a 2,000-fold dilution of the 10% abamectin suspension reached a control efficacy of 98.13% at 5 days post-application., significantly surpassing the performance of the other eight agents tested. Furthermore, in the management of T.truncatus in cotton fields, the 3-day application of 10.5% Abamectin pyridaben Micro-emulsion and 22.4% spirotetramat ethyl ester Suspension Concentrate presented exceptional results. Among them, 2500-fold dilution of the 10.5% Abamectin pyridaben microemulsion yielded a control efficacy of 91.16%, significantly outperforming the remaining seven drugs evaluated. 【Conclusion】 It is recommended that at the onset of cotton mite occurrence, a rotation of four acaricides in production is used to control T.dunchuangensis: 40% Phenthoate Fenobucarb Emulsifiable concentrates, 24% Bifenazate spiralate Suspension Concentrate, 30% Tolfenpyrad Suspension Concentrate, and 10% Avermectin Suspension Concentrate. For the control of T.truncatus, a rotational use of 10.5% Abamectin pyridaben Micro-emulsion and 22.4% spirotetramat ethyl ester Suspension Concentrate is suggested.