Abstract: 【Objective】 To explore the suitable pretreatment and drying methods by improving the utilization rate of leaves in the threshing process of Seabuckthorn branches,The findings provide theoretical basis and data support for the development and utilization of processing by-products of large fruit Seabuckthorn leaves in production. 【Methods】 In this study, Seabuckthorn leaves were used as test materials, and natural drying without pretreatment was used as control (CK). On the basis of systematically evaluating the drying effects of ultrasonic (CS) pretreatment, blanching (TP) pretreatment, ultrasonic+blanching (CS+TP) pretreatment and osmotic dehydration (ST) pretreatment on Seabuckthorn leaves, combined with natural (ZR) drying, hot air (RF) drying, microwave (WB) drying and vacuum freezing (LD) drying methods, the effects of different pretreatment combined with drying methods on the drying efficiency and bioactive substances of Seabuckthorn leaves were investigated. 【Results】 The results showed that the effect of CS pretreatment was significant after natural drying of Seabuckthorn leaves with different pretreatments. CS pretreatment combined with ZR, RF, WB and LD drying had significant effects on the drying efficiency and bioactive substances of H.rhamnoides leaves. Among them, CS pretreatment combined with RF drying method had the best effect, which could shorten the drying time of H.rhamnoides leaves by 4 times compared with CK, and meanwhile maintained the contents of total flavonoids, total phenols, proteins, polysaccharides and quebrachitol at 41.07, 29.56, 30.66, 3.53 and 22.14 mg/g, respectively. 【Conclusion】 Using CS pretreatment and RF drying method for large-scale utilization pretreatment of large fruit seabuckthorn leaves.