Abstract: 【Objective】 To explore the residual digestion patterns of different plant growth regulators such as gibberellic acid (GA₃), brassinolide (BR), and thidiazuron (TDZ) on jujube and their effects on fruit flavonoids and phenolic compounds in the hope of providing a theoretical basis for the scientific and standardized use of some plant growth regulators. 【Methods】 18 g/hm² and 36 g/hm² GA₃ were applied by foliar spray at the full bloom stage of jujube, and they were sprayed simultaneously with BR (45 mg/hm²) and TDZ (1.8 mg/hm²). Meanwhile, ultra-high performance liquid chromatography tandem mass spectrometry were used to determine the residual levels of GA₃ and TDZ in leaves after application, and analyzed the changes in flavonoid and phenolic acid content in fruits based on extensive targeted metabolomics analysis. 【Results】 The residual amounts of GA₃ at two kinds of doses on junjube were 0.10 mg/kg and 0.20 mg/kg, respectively, 14 days after application. The residual amount of TDZ was 0.01 mg/kg, which was lower than the MRL value. The digestion rates of GA₃ and TDZ on junjube were both above 90%. The half-lives of the two doses of GA₃ were 3.73 days and 4.26 days, respectively, while the half-lives of TDZ was 1.63 days. After treatment with GA₃, BR, and TDZ, the flavonoid compounds such as catechin gallate, epicatechin gallate, isoquercetin, rutin, and quercetin in junjube fruit were upregulated; Phenolic acid compounds such as benzoic acid, phenyl salicylate, phenylethyl caffeic acid, cinnamic acid were upregulated, too. 【Conclusion】 GA₃ and TDZ degrade rapidly on junjube, and the application of appropriate amounts of GA₃, BR, and TDZ helps to increase the content of flavonoids and phenolic acids in the fruit.