Abstract: 【Objective】 To study the effects of different chemical growth regulators on the physiological and biochemical indicators of the beating melon. A scientific basis for the in-depth study of the physiological and biochemical characteristics of seed melon, the enhancement of its yield as well as the improvement of plant shape. 【Methods】 The physiological and biochemical indexes of seed melon leaves were measured in 3, 6, 9, 12 and 15 d with different chemical growth regulators at 0.005% and 0.01% concentration, respectively by using the leaves of beating melon "common red large" as the test material. 【Results】 Under different concentrations and types of chemical growth regulator treatments, all the measured indexes showed different degrees of differential changes with the increase of time. The MDA content of 0.01% chemical growth regulator treatment reached the maximum value at 3 d. With the increase of chemical growth regulator treatment concentration and time, the soluble protein content could reach the maximum value of 370.2 mg/g. The H₂O₂ content of fluralin at 0.01% concentration reached the peak value of 8.83 μmol/g at 6 d; and the POD activity increased significantly and reached the maximum value of 1,156.6 U/g at 6 d under the 0.01% fluralin treatment. The POD activity increased significantly and reached the maximum value of 1,156.6 U/g under different chemical growth regulators and different concentration treatments. SOD had high activity at 3-6 d with different concentrations of chemical regulators, and then decreased with not significantly change. The lowest CAT activity at 15 d was 294.7 U/g under 0.01% vinlide treatment. 【Conclusion】 The MDA content, soluble protein content, H₂O₂ content, and peroxidase activity of beating melon were significantly increased after treatment with high concentrations of chemical regulators compared to low concentrations. In addition, with the prolongation of the treatment time, all the measured indexes show significant differences.