Abstract: 【Objective】 To study the subcellular localization of GmNF-YC4,which lays the foundation for revealing the molecular mechanism of soybean stress resistance. 【Methods】 In this study, GmNF-YC4 was cloned from soybean, bioinformatics for GmNF-YC4 sequence and phylogenetic tree, and the subcellular localization of GmNF-YC4 in tobacco cells was analyzed by transient transformation tobacco. 【Results】 The open reading frame size of GmNF-YC4 (GenBank accession number: NM _ 001362631.1) was 690bp, encoding 229 amino acids, which predicted molecular weight of 24.78 kDa, and isoelectric point of 5.19. The GmNF-YC4 protein contained a HAP 5 conserved domain. The phylogenetic tree analysis showed that GmNF-YC4 and AtNF-YC 1, AtNF-YC 4 were clustered in one clade. The genome of GmNF-YC4 contained 1 exon. The subcellular localization results indicated that GmNF-YC4 protein was localized in the nucleus of tobacco. 【Conclusion】 GmNF-YC4 is a nucleus-localized transcription factor. GmNF-YC4 gene sequence is obtained.