Abstract: 【Objective】 Analysis of expression patterns of glycometabolic pathway genes in sorghum anti-aphid response. 【Methods】 The aphid resistant variety HN16 and its sensitive aphid mutantasm1 were used as research materials and the transcriptomic database of sorghum was used to screen the differentially expressed genes (DEGs) of the two materials in the feeding process of aphids. In the end, a total of 17 genes were screened. These results suggested that feeding by aphids changed the expression of glycometabolic pathway genes in HN16 and asm1. Six pairs of specific primers of six genes (sbGOLS2, sbGUX2, sbCHIT3, sbBGL22, sbISOA3) were selected by PCR and qPCR for gene expression verification and analysis. 【Results】 The results showed that the expression patterns of these 6 genes were basically consistent with the transcriptome data, and they played different roles in the anti-insect response of HN16. Among them, the genessbGOLS2, sbCHIT3 andsbBGL22 were resistant to insects through rapid response to avoid excessive energy consumption. sbGUAA2, sbGUX2 andsbISOA3 ensured the stability of glycometabolic metabolism of sorghum by maintaining relatively stable expression during aphid feeding. 【Conclusion】 In summary, during the feeding process of aphids, HN16 maintains a relatively stable glycometabolic metabolism level by regulating the expression of glycometabolic metabolism pathway genes to resist the harm of aphids.