甜瓜蚜传病毒病(CMV、WMV和CABYV)发生规律分析

Analysis of occurrence law of Aphid-borne virus diseases (CMV, WMV and CABYV) of muskmelon

  • 摘要: 【目的】 系统调查新疆代表性甜瓜产区三种主要甜瓜蚜传病毒病发生状况,探明新疆甜瓜蚜传病毒病发生规律,为制定甜瓜病毒病防控措施提供依据。 【方法】 鉴定喀什地区伽师县田间有翅蚜和甜瓜样本中携带病毒种类;动态监测田间有翅蚜数量以及田间甜瓜植株发病率情况,采用分子生物学检测技术解析CMV、WMV和CABYV三种蚜传病毒病的田间发生规律,对田间采集到的209 份甜瓜样本进行RT-PCR检测。 【结果】 CMV平均检出率为53.62%,WMV平均检出率为44.38%,CABYV平均检出率为35.97%;对270 份有翅蚜为CMV的平均检出率为19.75%,WMV平均检出率为16.38%,CABYV平均检出率为13.95%;田间有翅蚜数量与甜瓜发病率呈正相关(P=0.40);气温与田间有翅蚜数量、甜瓜发病率呈正相关(有翅蚜数量P= 0.41, 发病率P= 0.75);相对湿度、降水量、地面风速与甜瓜发病率呈负相关(湿度P=-0.56,降水量P=-0.72,地面风速P=-0.38)。 【结论】 CMV、WMV以及CABYV三种病毒在新疆喀什地区伽师县的甜瓜植株和有翅蚜体内均被检出,均以CMV检出率最高;有翅蚜数量与甜瓜发病率呈正相关,有翅蚜迁飞到甜瓜田传毒后,带毒甜瓜有个隐症过程,隐症期为7~15 d;平均气温与田间有翅蚜数量、甜瓜病毒病发病率呈正相关。

     

    Abstract: 【Objective】 To systematically investigate three kinds of melon aphid-borne viral diseases in representative melon production areas of Xinjiang in order to explore the incidence of melon aphid-borne virus diseases,this study findings can provide some guidance to formulate scientific prevention and control measures of melon virus diseases. 【Methods】 Molecular techniques were used to identify the virus species in the collected winged aphid and melon plant samples. The incidence of CMV, WMV and CABYV aphids were determined by the number of aphids and the incidence of a disease in the field. 209 melon samples collected in the field were detected by qRT-PCR. 【Results】 The results showed that the average detection rates of CMV, WMV and CABYV were 53.62%, 44.38% and 35.97% respectively. The average detection rates of CMV, WMV and CAMYV of the 270 samples were 19.75%, 16.38% and 13.95% respectively. The number of aphids in the field were positively correlated with the incidence of a disease (P = 0.40). Air temperature was positively correlated with aphid number and incidence of a disease (aphid number P = 0.41, incidence P = 0.75). The incidence of a disease was negatively correlated with moisture, amount of precipitation and surface wind speed (humidity P =-0.56, precipitation P = -0.72 and ground wind speed P = -0.38). 【Conclusion】 CMV, WMV and CABYV are all detected in melon plants and winged aphids in Jiashi County of Kashi, and the detection rate of CMV is the highest. The number of winged aphids is positively correlated with the incidence of a disease. The mean air temperature is positively correlated with the number of winged aphids and the incidence of a disease.

     

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