Abstract: 【Objective】 This project aims to develop new KASP-SNP primers to reveal the differences in genetic structure among Puccinia striiformis f. sp. trititc populations in different endemic regions. 【Methods】 On the basis of existing SNP loci, we screened SNP loci with suballele frequency > 0.4 and designed KASP-SNP primers to analyze the genetic structure and primer polymorphisms of spring and winter wheat Puccinia striiformis f. sp. trititc in the Ili River Valley. 【Results】 Eighteen pairs of KASP-SNP primers with stable amplification and obvious genotyping effect were finally selected, there were certain genetic differences between the spring and winter wheat stripe rust populations in the Ili River Valley, and the polymorphic information content index of the 18 pairs of primers averaged 0.273,5, and the gene diversity index averaged 0.332,5, which was higher than the SSR average diversity index. 【Conclusion】 The 18 pairs of KASP-SNP primers developed in this study are rich in polymorphism and can be used to analyze the population genetic structure of Puccinia striiformis f. sp. trititc.