Abstract:
【Objective】 To discuss the adaptation of
S.tianschanica Rupr. to light intensity by analyzing the leaf stomata characteristics and photosynthetic physiological changes of
Sorbus tianschanica Rupr. seedlings under different light intensity, in the hope of providing reference for the conservation and domestication of
S.tianschanica Rupr. seedlings.
【Methods】 Two year seedlings of
S.tianschanica Rupr. were used as materials, and four light gradients (L
1:50%, L
2:30%, L
3:10% and CK: 100%) were set up by sunshade nets with different number of needles to conduct pot experiments, and the changes of stomata characteristics, photosynthetic fluorescence parameters and chlorophyll content of
S.tianschanica Rupr. seedlings under different light intensities were analyzed.
【Results】 The seedlings of
S.tianschanica Rupr. had the best growth under L
2 treatment, and their seedling height and ground diameter were the largest. The results showed that with the decrease of light intensity, the stomatal density of
S.tianschanica Rupr. leaves gradually increased and there were significant differences among different treatments (
P < 0.05), but the changes of leaf stomatal length, width, perimeter, area and opening under different light conditions were not significant (
P > 0.05). The
Pn,
Gs and Tr of
S.tianschanica Rupr. seedlings increased first and then decreased with the decrease of light intensity, and
Ci increased gradually. Under L
2 treatment, the
Pn of the leaves was the highest, 17.07 μmol/(m
2·s). The
Fv/Fm of the leaves increased with the decrease of light intensity, and the
Fv/Fm of the leaves under all treatments was significantly higher than that under CK treatment (
P < 0.05). The
ETR of leaves reached the highest level in L
2 treatment, and there were significant differences in
ETR of leaves between treatments (
P < 0.05). The diameter of
ФPSⅡ of the leaves was L
1 > L
2 > CK > L
3. The contents of Chl
a, Chl
b, Chl
t and Car increased with the decrease of light intensity, and Chl
a/b decreased with the decrease of light intensity. Light intensity was positively correlated with
Pn and
Tr (
P < 0.01), and
Fv/Fm was positively correlated with
ETR, Chl
a, Chl
b and Chl
t contents (
P < 0.01).
【Conclusion】 The results show that the light intensity of L
2 treatment (light intensity of 30%) has more potential to promote photosynthesis and growth of
S.tianschanica Rupr. seedlings. moderate shading should be carried out to improve the quality of seedlings and introduce and cultivate them.