Abstract: 【Objective】 To study the effects of sodium selenite, nano-selenium and organic selenium on the appearance, antioxidant capacity and selenium metabolism of Hui jujube in the hope of providing theoretical basis for analyzing the accumulation mechanism of different selenium forms in plants. 【Methods】 Three treatments, sodium selenite, nano-selenium and organic selenium, were set up to determine the appearance of leaves and fruits, antioxidant enzyme activity and gene expression levels, selenoamino acids and gene expression levels related to selenium metabolism before and after treatment. 【Results】 After the application of nano-selenium, sodium selenite and organic selenium, the appearance morphology, antioxidant enzyme activity and gene expression, selenoamino acid content and selenium metabolization-related gene expression levels of Hui jujube were significantly increased, among which the hardness of Hui jujube increased by 14.40%-19.59%, soluble solid increased by 23.77%-37.43%. Glutathione peroxidase (GSH-Px) and gene expression were increased by 10.23%-110.41% and 8.18%-123.91%, respectively, total selenium content was increased by 3.09-34.14 times, selenometine (SeMet) was increased by 100.00%-346.15%. Cysteine desulphurase (CysD) gene expression increased by 18.13%-195.43%. 【Conclusion】 After nano-selenium, sodium selenite and organic selenium are transformed into selenoamino acids after spraying, different forms of selenium have effects on the appearance, antioxidant capacity and selenoamino acids of Hui jujube. In conclusion, organic selenium treatment is superior to sodium selenite and nano-selenium treatment.