Abstract: 【Objective】 To investigate the presence of Staphylococcus aureus contamination in a geese slaughterhouse located in southern Xinjiang by analyzing the virulence genes, antibiotic resistance, and typing of the isolated strains. The investigation results of pollution in different links are helpful to analyze the key control points of pollution in slaughtering and processing links, and can effectively prevent and control the outbreak of foodborne diseases. 【Methods】 A total of 153 samples (including swabs and meat samples taken from knives, workers' hands, production equipment, goose carcasses) were collected in the goose slaughterhouse. The isolation and identification of Staphylococcus aureus were achieved by combining traditional culture methods with PCR technology. Simultaneously, 12 enterotoxin genes were identified, along with the detection of staphylococcal protein A. The resistance of 12 types of antibiotics was investigated using the AGAR dilution method. 【Results】 From this goose abattoir, a total of 49 strains of S. aureus were isolated and identified, resulting in an isolation rate of 32.0%. The highest isolation rate of 40.0% (22/55) was found in goose carcasses, followed by 36.0% (9/25) in worker's hands, 34.3% (12/35) in production utensils, 20.0% (2/10) in knives, and 14.3% (4/28) in goose meat. All isolates consisted of seven clonotypes (t078, t034, t502, t701, t1376, t002, and t091) and three unidentified subtypes. Seven enterotoxin genes were identified, with seb (26.5%), seg (46.9%), sei (32.7%), sem (24.5%), and seu (28.6%) genes being commonly discovered. The drug sensitivity results revealed that 49 strains of S. aureus exhibited multi-drug resistance, with resistance rates to ampicillin, azithromycin, sulfisoxazole, and ciprofloxacin ranging from 73.50% to 49.0%. The multi-drug resistance was mainly triple and quadruple resistance, and the highest was seven-fold resistance. 【Conclusion】 Staphylococcus aureus is seriously contaminated in goose slaughterhouses, and cross-contamination occurs in different processing links. The contamination rate of goose carcasses is the highest, which accounts for 40.0%. The isolates are mainly classified as t078, t034 and t502. Staphylococcus aureus isolates carries a variety of enterotoxins, and 63.3% of isolates show multiple drug resistance.