葡萄双生病毒A的序列分析及检测
Sequence analysis and detection of grapevine geminivirus A
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摘要: 【目的】 研究新疆吐鲁番市葡萄双生病毒A(Grapevine geminivirus A)的田间侵染状况,检测采集71份疑似葡萄病毒样本,为明确葡萄双生病毒A的发生、分布及其分子检测提供理论指导。 【方法】 依据高通量测序结果,验证样本中葡萄双生病毒A,测定GGVA的全基因序列,以葡萄双生病毒A的全基因序列为靶标设计特异性引物进行疑似葡萄病毒样本的检测。 【结果】 测定GGVA的全基因组序列,获得GGVA-QKT分离株,其核苷酸同源性为94.3%~99.3%,与已公布的分离株具有高度同源性,其中与来自以色列(KX618694.1)、(NC_031340.1)的2个GGVA分离株亲缘关系最近。 【结论】 71份不同葡萄样本均有感染双生病毒A,其中,新疆鄯善县检出率最高,检出率为30.55%,新疆托克逊县检出率最低,检出率为9.10%。该病毒在新疆吐鲁番市葡萄种植区普遍发生。Abstract: 【Objective】 This project aims to determine the field infection status of Grapevine Geminivirus A in Turpan City,Xinjiang.【Methods】 71 suspected grape virus samples were collected and detected and according to the results of high-throughput sequencing, the existence of grapevine geminivirus A in the sample was verified, and the whole gene sequence of GGVA was determined. The whole gene sequence of grapevine geminivirus A was used as the target to design specific primers for the detection of suspected grape virus samples. 【Results】 The whole genome sequence of GGVA was determined, and GGVA-QKT isolates were obtained, with nucleotide identity of 94.3%-99.3%, and published isolates were highly homologous, among which, they were closely related to two GGVA isolates from Israel (KX618694.1) and (NC_031340.1). 【Conclusion】 71 different parts of the grape samples were Grapevine geminivirus A, 71 grape samples from different regions were infected with Grapevine geminivirus A. among them, Shanshan County,Xinjiang had the highest detection rate 30.55%, while Tokson County,Xinjiang had the lowest detection rate 9.10%. The virus was very common in the grape growing area of Turpan County,Xinjiang. In this study, the field infection status of Grapevine geminivirus A in Turpan City is detected.
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